CLTC(Clathrin Heavy Chain 1)是一种"星月蛋白",在细胞有丝分裂间期执行膜泡运输的功能,而在分裂期对纺锤体的稳定和染色体在赤道板上的聚集发挥重要作用.但是,CLTC在小鼠卵母细胞减数分裂中的作用机理仍然不是很清楚.本研...
详细信息
CLTC(Clathrin Heavy Chain 1)是一种"星月蛋白",在细胞有丝分裂间期执行膜泡运输的功能,而在分裂期对纺锤体的稳定和染色体在赤道板上的聚集发挥重要作用.但是,CLTC在小鼠卵母细胞减数分裂中的作用机理仍然不是很清楚.本研究旨在探讨CLTC在小鼠卵母细胞减数分裂及早期胚胎发育过程的表达、定位及功能.研究结果表明，CLTC在小鼠卵母细胞减数分裂过程中对纺锤体的形成、染色体的连接、纺锤体检验点的功能发挥重要作用。CLTC影响纺锤体组装和染色体赤道板集合的分子机制需要进一步研究。
Glucose transport, mediated by glucose transporters, is necessary for mammary gland development and ***1 and GLUT12 could both be expressed in the pregnant and lactating mammary gland to participate in theglucose upta...
详细信息
Glucose transport, mediated by glucose transporters, is necessary for mammary gland development and ***1 and GLUT12 could both be expressed in the pregnant and lactating mammary gland to participate in theglucose uptake process. In this study, the goat GLUT1 and GLUT12 genes were cloned from Saanen dairy goatsand transfected into goat mammary gland epithelial cells to assess their biological functions and distributions. Theresults showed that both goat GLUT1 and GLUT12 had 12 predicted membrane-spanning helices. Goat GLUTIand GLUT12 each influenced the mRNA expression of the other transporter and increased the glucose consumptionand lactose yield in GLUT1一and GLUT12-transfected goat mammary gland epithelial cells, respectively. Theoverexpression of GLUT1 or GLUT12 also increased the expression of amino acid transporters SLC1A5, SLC3A2and SLC7A5 and affected genes expressions in GMGE cells. Using immunofluorescence staining, GLUT1 wasdetected throughout the cytoplasm and localized to the Golgi apparatus around the nuclear membrane, whereasGLUT12 was mainly distributed in the perinuclear region and cytoplasm. This study contributes to theunderstanding of how GLUT1 and GLUT12 cooperate in the incorporation of nutrient uptake into mammary glandepithelial cells and the promotion of milk synthesis in the goat mammary gland during lactation.
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