Sperm sorting for sex preselection is one of the major achievement in reproductive biotechnology that hasgreatly influenced worldwide dairy ***,semen is labeled with a fluorescent by Hoechst 33342 whichbinds to the DN...
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Sperm sorting for sex preselection is one of the major achievement in reproductive biotechnology that hasgreatly influenced worldwide dairy ***,semen is labeled with a fluorescent by Hoechst 33342 whichbinds to the DNA of each spermatozoon,and then exposed to UV light during flow cytometry to measure theamount of fluorescent *** the X-chromosome is larger than the Y chromosome,X and Y sperm will be sorted interms of the difference of the DNA content by computer *** high speed sperm sorter,SX-MoFlo flowcytometer was developed for commercial purpose and licensed to XY Inc,in 2000,which be able to sort X or Y sperm at a rate of 5000-7000 per second and could obtain greater than 90％ ***,the number of sortedsperm to produced sexed semen only 30-50 thousand doses per sorter each year which still limits *** developed a new technology for sexed semen production in dairy cattle based on SX-MoFlo flowcytometer and mix the sorted sperm with xenosemen which can improve more than two times of sexed semenproduction compared to original ability to 60-80 thousand doses per sorter each *** 2005,over 1.4 milliondoses of the sexd dairy cattle X-semen were applied for AI in China,which resulted 50-60%conception rate ofeach sex cycle and 94% heifers of average sex preselection rate in the sexed *** results indicatedthat feiled application of sex controling by sorted X-semen and AI is feasible with both of our developedtechnology and new product quality for dairy industry in China.
High-throughput RNA sequencing was performed for comprehendsively analyzing the transcriptome of thegeese.A total of 28,803,759 by of raw sequence data were generated,of which 28,730,361bp remained afteradaptor trimmi...
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High-throughput RNA sequencing was performed for comprehendsively analyzing the transcriptome of thegeese.A total of 28,803,759 by of raw sequence data were generated,of which 28,730,361bp remained afteradaptor trimming (99％ of sequenced bases).After removal of adaptor sequences,117,279 reads were obtained,with an average length of 244 *** ofall the reads from both 454 and illumine platforms,formed56,839 contigs,with 12,995 readsremaining as singletons,Ranging from 38 to 28206 by in size,with an averagesize of 2584 by and an N50 of *** assembly produced a substantial number of large contigs:35,545 (62.5%)were longer thanlkb,of which 8850 15.6%)were longer than *** sequencing depth was 85 X on *** comprehensive function annotations on unigenes including protein sequence similarity,GO termclassification,and KEGG pathway *** ontology (GO) analysis showed that approximately 63% ofthe contigs had annotation information,among the 36,756 annotated sequences in Nr database,24,299(66.1%)sequences were assigned with one or more GO *** were 19,757(81.3%)isotigs for biological processes,10,643(43.4%)isotigs for cellular component,22818 (93.9%) isotigs for molecular *** result of KEGGpathway mapping 8,737 sequences had the pathway annotation,and took part in477 *** addition,different expression genes in ovarian tissue between different states of Sichuan White geese were detected in thecurrent study.
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