Introduction:In the long period of natural selection and artificial selection,people began to plant alfalfa for cattle feed on theGuanzhong Plain,the main production area of Qinchuan beef *** resulted in tremendous i...
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Introduction:In the long period of natural selection and artificial selection,people began to plant alfalfa for cattle feed on theGuanzhong Plain,the main production area of Qinchuan beef *** resulted in tremendous improvements inQinchuan beef cattle,particularly in relation to its body size,work ability,and individual meat *** of thebeef cattle transcriptome and its expression profile data are essential to extending the genetic information resourcesfor this species and would support studies on this *** and Methods:Using RNA-Seq,this study compared the transcriptomic landscapes of longissimus muscle from embryo at day 135 post fertilization versus 30-month-old adult ***:A total of 25605140 and 26214800 reads were sequenced for embryonic and adult pooled samples,*** identified 24464-29994 novel transcript units in two pooled *** addition,8533一10144 genes showedevidence of alternative splicing,in agreement with the finding that alternative 3'splicing is the most common typeof alternative splicing event in *** detected the expression levels of 16174 genes, and 6800 genes exhibiteddifferential expression between the two pooled samples with a false discovery rate≤*** GO enrichmentand ICEGG pathway analysis,multiple GO term and biological pathways were found to be significantly enrichedfor differentially expressed *** addition,we discovered that 30618-31334 putative single nucleotidepolymorphisms were located in coding ***:A majority of the annotated transcripts in the Ensembl database were covered by sequenced reads,illustrating thesensitivity of RNA-seq for transcript discovery,even for genes expressed at low *** also found thatalternative 3'-end splicing is the most common type of alternative splicing event in cattle and that intron retention isthe least common alternative splicing *** results are consistent with those of a similar pig ***,these res
Porcine induced pluripotent stem cells (piPSCs) have significant biomedical and agricultural applications andhave been developed by several laboratories *** piPSC line has shown varying levels of heterogeneityin morph...
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Porcine induced pluripotent stem cells (piPSCs) have significant biomedical and agricultural applications andhave been developed by several laboratories *** piPSC line has shown varying levels of heterogeneityin morphology,self-renewal and ***,questions have been raised pertaining to incompletereprogramming of piPSCs,the plasticity of these cells and the necessary gene regulatorynetworks that are needed to maintain *** address these questions and to better understand themechanism of porcine cell reprogramming,we analyzed the transcriptional profiles of porcine iPSC lines usingAffymetrix GeneChip Porcine Genome *** results demonstrated that cell surface proteins of EpCAM(epithelial cells adhesion molecule) and Rexl were significantly upregulated in complete reprogrammed porcineiPSCs,but not in partially reprogrammed cells,suggesting that these genes could be markers for evaluating porcinecell *** analyzed gene expression levels of the six key developmental signaling pathways,including JAK-STAT,NOTCH,TGF-b,WNT,MAPK and *** results demonstrate that the coretranscriptional network to maintain pluripotency and self-renewal in pig may be different from mouse and *** iPSCs lacked expression of specific naive state marker,but expressedunregulated primed state *** global networks of pig iPSCs were dominated byhighly connected genes such as POUSFI,LIN28A,DNMT3b,ERK and MAPK,which are possibly critical indetermining the fate of ***-Dio3 domain was silenced in piPSCs as previously seen in mouse and humaniPSCs,which is a likely cause of low instances of chimeric and cloned *** analysis provides afundamental resource for better understanding of the complex genetic network that maintains pluripotency in *** comparative study with mouse and human may facilitate the derivation of iPS lines from pig moreefficiently.
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