Objective Fabricate a heat-sensitive microbubble(HSM) agent by using a capillary co-flow focusing method(CFF),assess the effects of the main process parameters,and further evaluate the performance of HSMs-assisted...
Objective Fabricate a heat-sensitive microbubble(HSM) agent by using a capillary co-flow focusing method(CFF),assess the effects of the main process parameters,and further evaluate the performance of HSMs-assisted identifying ablation margins in *** and Methods Hydrogenated L-α-phosphatidylcholine was dissolved in chloroform to form a homogeneous *** lipid suspension was prepared by mixing 0.2 g L-a-phosphatidylcholine,2.1 g Tween 80,12.6 g glycerol,0.5 g polyethyleneglycol(PEG) 200 stearate,10 g propane-1,3-diol,and 80 g distilled water(the mass ratio2:21:126:5:100:800).DiI is dissolved in the lipid solution at a concentration of 0.01 mg ml-1.1,1,1,2,3,4,4,5,5,5-Decafluoropentane(a PFC compound with boiling point of 55℃).The CFF system includes a droplet generation module,a droplet collection module,and a process monitoring *** droplet generation module includes a CFF atomizer,a compressed gas storage,a manometer and a twin syringe *** geometrical parameters can be adjusted in *** dynamic behavior of the coaxial cone-jet structures can be monitored in real time and stored into data files for post *** shell material was stained with DiI dye and imaged by a *** morphology of the microbubbles was observed by means of an optical microscope and the size distribution of microbubbles was determined by an image-processing *** diameter determination and the statistical analysis were carried out from various *** destruction of the HSMs was validated in a well-designed benchtop system.A membrane tube was mounted in the water bath with 1 cm below the water free *** ends of the tube were connected to an external circulation *** water temperature around the tube was measured by a thermometer in real *** imaging system was used to monitor the flow information inside the *** test results were compared with the control where only degassed water flew through the
Objective Triggered release of drugs from carrier systems in vivo is widely investigated for targeted tumor *** the past decade,an ultrasound-sensitive liposome-microbubble pendant drug carrier system has been develop...
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Objective Triggered release of drugs from carrier systems in vivo is widely investigated for targeted tumor *** the past decade,an ultrasound-sensitive liposome-microbubble pendant drug carrier system has been developed:microbubbles,decorated with liposomes that carry e.g.,doxorubicin,have been shown to release the drug in response to ***,due to low drug load,so far,those pendant particles were only successful for suppressing tumor cell growth in vitro,in cell culture *** this study,we were able to prepare particles with high doxorubicin load,using liposomes larger than the standard 80 nm Doxil/Caelyx formulation,and achieved successful inhibition of tumor growth in a subcutaneous murine tumor *** Decafluorobutane microbubbles were stabilized with a shell consisting of DSPC,PEG stearate and biotin-PEG-DSPE(20:20:1 mass ratio).Liposomes carrying ammonium citrate were prepared from DOPC,cholesterol and biotin-amidocaproyl-DSPE;liposomes were purified from external ammonium citrate by repeated *** loading of citrate-containing liposomes with doxorubicin from external HEPES buffer was performed at elevated temperatures,to achieve efficient drug entrapment inside the liposome inner aqueous *** linker was used to affix biotinylated liposomes onto the surface of biotinylated microbubbles,with pendant complex formation.C57 BL/6 mice were injected subcutaneously in the hind leg with MC38 colon carcinoma cells(a generous gift of ***,NIH),therapy has been initiated when tumors have reached 4~5 mm ***-liposome-microbubble complexes were injected intravenously(doxorubicin dosage 6 mg/kg);all animal studies were performed under isoflurane inhalation anesthesia,on a warm pad to maintain animal body *** ultrasound imaging with Sequoia 512(15 L8 probe,nondestructive CPS imaging,7 MHz,MI 0.2) was used to monitor the particles in the tumor *** ultrasound tre
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