Genome editing is valuable for understanding gene function and for gene *** the rate of traditionally homologous recombination is very low and the Cre/loxP leaves behind a 34 bp sequence together with the targeted mut...
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Genome editing is valuable for understanding gene function and for gene *** the rate of traditionally homologous recombination is very low and the Cre/loxP leaves behind a 34 bp sequence together with the targeted mutation and the transposase can reintegrate the released piggyback into other chromosomal *** we present a seamless strategy for editing CCR5 gene using the CRISPR-Cas9 system twice based on the HR and SSA repair mechanism *** the first targeting, up to 100% clones were targeted in CCR5 gene on one *** the second targeting, 2.08% clones were targeted and repaired by SSA repair mechanism resulting in a 32 bp fragment replaced by SalI enzyme site *** study provided a novel method for seamless genome editing and would promote the production for transgenic animals.
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