INTRODUCTION In biological researches, chicken embryos have become a classic experiment model for the exploration of the embryonic development and cell *** exogenous substances into chicken embryos for producing medic...
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INTRODUCTION In biological researches, chicken embryos have become a classic experiment model for the exploration of the embryonic development and cell *** exogenous substances into chicken embryos for producing medical antibodies has been widely used in the production ***, there was few studies about the diffusion effects of the different injection location on chicken *** aim of this study was to explore the diffusion effects of different injection location, as well as the the impacts of different injection dosage on chicken embryo hatching rate and development, so as to provide basis and direction for the further study of chicken embryo injection.
Somatic cell nuclear transfer (SCNT) has valuable application in the swine industry as well as bioscience research, but it has been circumscribed because of its *** reprogramming is considered one of the most importan...
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Somatic cell nuclear transfer (SCNT) has valuable application in the swine industry as well as bioscience research, but it has been circumscribed because of its *** reprogramming is considered one of the most important reasons leading to the death of SCNT *** have found that X-linked genes are remarkably down-regulated in mouse cloned embryo compared with in vitro fertilization (IVF) derived embryos.
Type Ⅱ CRISPR (clustered, regularly interspaced, palindromic repeats)/Cas9 (CRISPR-associated endonuclease 9) system has been exploited for locus-specificgenome editing in a wide variety of model organisms and genera...
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Type Ⅱ CRISPR (clustered, regularly interspaced, palindromic repeats)/Cas9 (CRISPR-associated endonuclease 9) system has been exploited for locus-specificgenome editing in a wide variety of model organisms and generated tremendous research upsurge due to certain characteristics-simplicity, efficiency and multiplex targeting ***, various assessments have shown thatunwanted mutations associated with off-target DNA cleavages could be the restriction element in certain applications.
Bovine tuberculosis prevails worldwide in livestock and wildlife except in ***, different susceptibilities between camels and other species have not been ***-interacting protein (TOLLIP) gene variants are associated w...
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Bovine tuberculosis prevails worldwide in livestock and wildlife except in ***, different susceptibilities between camels and other species have not been ***-interacting protein (TOLLIP) gene variants are associated with tuberculosis susceptibility in *** this study, differences in RNA and protein levels were separately analyzed;different TOLLIP gene polymorphisms were then compared among various *** indicated TOLLIP mRNA and protein structures varied except the conservative C2 domain in different *** protein structure indicated that the structure of the TOLLIP gene of camels differs from that of other species.
Vitrification has been widely employed to cryopreserve animal and human oocytes and *** can not only promote oocyte donation program but also preserve fertility in cancer patients who have to receive frequent radiatio...
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Vitrification has been widely employed to cryopreserve animal and human oocytes and *** can not only promote oocyte donation program but also preserve fertility in cancer patients who have to receive frequent radiation ***, oocyte vitrification still faces some challenge with relatively lower embryonic development ***, several studies have primarily focused on the consequences of vitrification and the development potential of cellular *** refers to certain heritable modifications primarily occurring in the DNA or chromosome, such as DNA methylation, histone modification, and genomic imprinting, without changing the DNA sequences.
Tuberculosis is an infectious disease caused by mycobacterium tuberculosis, which most commonly affects the lungs and causes over 1.3 million people die *** in host genes is known to influence susceptibility to *** of...
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Tuberculosis is an infectious disease caused by mycobacterium tuberculosis, which most commonly affects the lungs and causes over 1.3 million people die *** in host genes is known to influence susceptibility to *** of the Intracellular Pathogen Resistance 1 (Ipr1) gene could enhance the host resistance to *** we analyzed the coding region sequence and promoter of Ipr1 gene of mouse strains C57BL/6 and BALB/c.
Melatonin was an important indoles hormone secreted by pineal gland and had series of functions such as antioxidant, regulate the immune system, and *** generation of melatonin in the pineal gland was controlled by **...
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Melatonin was an important indoles hormone secreted by pineal gland and had series of functions such as antioxidant, regulate the immune system, and *** generation of melatonin in the pineal gland was controlled by *** most mammal animals, the synthesis of melatonin in pineal gland reached the peak in autumn and *** were important economic animal and its reproduction were *** this study, we collected testis tissue of male goat at different seasons, the analysis of histological section showed the spermatogenesis at seasonal estrous was stronger than the anestrous season.
Natriuretic peptide type C (NPPC) activation of the guanylyl cyclase-linked natriuretic peptide receptor 2 (NPR2) maintains oocyte meiotic *** hormone (LH)-dependent epidermal growth factor (EGF) receptor (EGFR) activ...
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Natriuretic peptide type C (NPPC) activation of the guanylyl cyclase-linked natriuretic peptide receptor 2 (NPR2) maintains oocyte meiotic *** hormone (LH)-dependent epidermal growth factor (EGF) receptor (EGFR) activation elevates calcium of cumulus cells to reduce NPR2 activity, resulting in meiotic *** study investigated the regulatory mechanism of elevated calcium on NPR2 inactivation in cumulus *** mouse ovarian follicles, the activation of LH receptor significantly elevated calcium concentration in cumulus cells, but decreased NPR2 binding affinity for NPPC and the phosphorylation levels.
a member of transforming growth factor β (TGF-β) family, mostly expressed in skeletal muscles and known to play a crucial role in the negative regulation of muscle *** (FSTN), also known as a suppressor protein of F...
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a member of transforming growth factor β (TGF-β) family, mostly expressed in skeletal muscles and known to play a crucial role in the negative regulation of muscle *** (FSTN), also known as a suppressor protein of FSH, is a single chain *** can combine with MSTN to block the function of MSTN, and promote muscle *** knockout or knockdown and over-expression of FSTN can induce the target animals with significant increase in muscle *** this experiment, we constructed interference vectors of MSTN and detected the interference efficiency, and obtained two interference vectors which can disturb the expression of MSTN with high efficiency.
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