Genetically modified (GM) livestocks have the potential to contribute to improving the environment and human health, with consumption of fewer resources and reduced waste ***, the transgene process also poses *** safe...
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Genetically modified (GM) livestocks have the potential to contribute to improving the environment and human health, with consumption of fewer resources and reduced waste ***, the transgene process also poses *** safety assessment and control of transgenic animal products have drawn wide attention, and the relevant regulations and technology are being *** testing technology plays a significant role in on-site and customs ***, loop-mediated isothermal amplification (LAMP) was widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency and *** this study, a specific, the sensitive detection system for detecting sFAT-1 transgenic pigs was designed.A set of 6 primers including 2 loop primers was designed for the target *** DNA samples were amplified in less than 1 hour at the optimized temperature and detecting by both Nephelometer LA-320c and unaided eyes directly adding *** detection limit of sFAT-1 LAMP was as low as 1.26 ng/μ***, blind tests of transgenic and non-transgenic DNA samples were all correctly ***, the results in this study demonstrated that LAMP is a very useful tool for transgenic detection.
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