Objective:Rat incisional wound model was widely used for the study of wound scarring and its manipulation. However,the limited volume of scar tissue in this model may limit its further application for scar manipulatio...
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Objective:Rat incisional wound model was widely used for the study of wound scarring and its manipulation. However,the limited volume of scar tissue in this model may limit its further application for scar manipulation *** study explores the possibility of increasing scar volume in a rat skin wound by inserting an absorbable gelatin *** Two full-thickness wounds were created in each of total 45 SD *** the first group of 15 rats,an incisional wound of 1cm long was created on the left side as a *** excisional wound of 1?0.2cm was created on the right side and a gelatin sponge of 1?0.3? 0.5cm was inserted as an experimental *** confirm the stability of this model,2 excisional wounds with sponge insertion were created in each of the second group of SD rats(n = 15).In the third group,2 excisional wounds(1? 0.2cm)were created in each of the rats,a gelatin sponge was inserted in right side and the left side left unrepaired as a *** of all three groups were sacrificed at days 7(n=5),14(n=5) and 21(n=5) to harvest wound tissues for gross and histological *** The scar width was much wider in experimental wounds than in control wounds grossly and histological at all three time points in the first group(P<0.05) with a thicker *** severe inflammation was also observed in the experimental wounds with a significant difference in infiltrated inflammatory cells between experimental and control wounds(P <0.05).In addition,a significantly higher expression level of TGF-β1、2 and TGF-βreceptorsⅠandⅡwas observed in the experimental wounds when compared with the control *** day 21,more mature collagen structure was observed in control wounds than in experimental *** contrast,there was no significant difference in scar width between two experimental wounds of second *** the third group,the control side exhibited an irregular contracted *** A reliable scar model with a much wider scar width,s
Introduction The mec hanisms of keloid invasiveness are largely *** TGF-beta plays very important roles in keloid pathogenesis,whether it plays a role in promoting keloid invasion remains *** on the clinical observati...
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Introduction The mec hanisms of keloid invasiveness are largely *** TGF-beta plays very important roles in keloid pathogenesis,whether it plays a role in promoting keloid invasion remains *** on the clinical observation of keloid lesion that includes peripheral invasive area and central regressive area that is not invasive,we hypothesized that there will be differential gene expression between the fibroblasts of these two areas and thus may find a molecule responsible for keloid invasion. Methods Keloid,hypertrophic scar or donated normal skin tissues were obtained from patients who underwent plastic surgery and received no previous *** of three keloids were dissected into peripheral "invasive area" and central "regressive area" respectively based on gross morphology and digested to harvest *** was extracted from the first passage cells and subjected to a microarray chip analysis that contains total 96 genes related to TGF-beta signaling and *** addition,another 3 keloid along with 3 hypertrophic scar tissue and 3 normal skin tissues were digested with collagenase for cell harvest and RNA *** expression of GDF -9 was evaluated with real-time *** addition,these tissues were also frozen sectioned for immunofluorescence staining of GDF-9 expression. Results The chip analysis revealed differential gene expression pattern between the "invasive areas" and "regressive areas".Of the differentially expressed genes, the expression of GDF -9,a member of a TGF -beta superfamily,was significantly higher in all 3 tested "invasive areas" than their respective "regressive areas" with an average of 4.66 fold *** addition,this differential expression of GDF -9 was also confirmed by real -time ***,real -time PCR analysis revealed a 300 times more increase of GDF-9 expression in keloid than in hypertrophic scars and normal skins. Furthermore,immunofluorescence showed high protein expression of GDF
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