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Treating organic cyanide-containing groundwater by immobilization of a nitrile-degrading bacteriumwith abiofilm-forming bacterium using fluidized bed reactors

Treating organic cyanide-containing groundwater by immobiliz...

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第二十次全国环境微生物学学术研讨会

作者： XuejiaoAn Yi Cheng Mingyan Huang Yueling Sun HailanWang Xi Chen JinmingWang DapengLi ChunyanLi College of Resources and Environment Northeast Agricultural University Harbin 150030Heilongjiang College of Science China Agricultural University Beijing 100083 PR China

Organic cyanide are widely used asan ingredient in the production of plastics,synthetic rubbers,polymers,pharmaceuticalsand pesticides or used in laboratories and industries as *** nitrile-containingwastewateris subjected toprimary and secondary treatments,residual nitriles may slowlyseep and further migrate through groundwater,resulting in the micropollution ofgroundwater byorganic *** this study,water samples were collected from different study areas in North China during a period of three years (from 2013 to 2015) and analyzed to evaluate organic cyanide (CN) contamination in *** parallel lab-scale FBRs were tested for their ability to remove organic cyanide from *** organic cyanide concentration in groundwater increased significantly (P<0.05) from 2013 to *** an optimal hydraulic residence time (HRT) of 54 min,reactor R3 (inoculated with a nitriledegrading bacterium,BX2,and a biofilm-forming bacterium,M1) effectively removed 99.84% of CN under steady operation,which was better than that of other ***-term shutdowns of FBRs had no serious effects on the efficiency ofireating organic *** work demonstrated that the biofilm-forming bacterium could facilitate the fixation of nitrile-degrading bacterium and enhance the efficiency of removing organic cyanide from groundwater.

关键词： Water quality Rhodococcus rhodochrous BX2 Biofilm Immobilization Organic cyanide Groundwater treatment

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A novel environmental fate of graphene oxide: biodegradation by a soil bacterium Labrys ***

A novel environmental fate of graphene oxide: biodegradation...

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第二十次全国环境微生物学学术研讨会

作者： Jingwei Wang Shengyang Lian Xinyi Liu Yongquan Yan Zifan Qiao Yuanyuan Qu Key Laboratory of Industrial Ecology and Environmental Engineering (MOE) School of Environmental Science and Technology Dalian University of Technology Dalian 116024 China

Graphene oxide (GO) is a new type of nanomaterial with unique physicochemical properties and diverse applications,while it poses potential risk to human and *** screening from natural soil exposed to GO in the laboratory,we successfully obtained a novel bacterium,Labrys ***,which was able to use GO as sole carbon source for *** 8 days,cell numbers of strain WJW increased to 16.76±3.21 folds using 100 mg/L *** bacterial biodegradation which resulted in formation of holes in the basal plane and functional group changes of GO was proved by Raman spectroscopy,atomic force microscopy,transmission electron microscopy,X-ray photoelectron spectroscopy,and thermogravimetric *** intermediates with structure of benzoic and phenol were identified using gas chromatograph-mass spectrometry and liquid chromatography/time-of-flight/mass *** of genomic and proteomic analyses were performed to explore and identify key enzymes for GO degradation.644 proteins were significantly *** using bioinformatics approaches,the results revealed that part of the upregulated proteins were related to oxidation,ring cleavage and intermediates transmembrane processes,and GO was supposed to be degraded to benzoate and then further degraded for downstream *** study enriches our understanding and provides new insights into the environmental fate of GO.

关键词： Graphene oxide Labrys sp.WJW Biodegradation proteomic

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Rhizosphere dialogue in the Radix pseudostellariae rhizosphere under continuous monoculture regimes

Rhizosphere dialogue in the Radix pseudostellariae rhizosphe...

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第二十次全国环境微生物学学术研讨会

作者：吴红淼林文雄福建省农业生态过程与安全监控中重点实验室福建农林大学福州350002 作物生态与分子生理学福建省高校重点实验室福州350002

The root exudates of Radix pseudostellariae,working as a chemical language between plants and microbes,can deteriorate the microbial community in the rhizosphere in a consecutive monoculture *** assessed the effects of artificially applied *** root exudates on *** seedling growth,rhizosphere soil microbial communities,and soil physicochemical *** found the effect of phenolic acids and organic acids acted as a driver of changes to the microbial *** throughput sequencing and qRT-PCR analysis demonstrated that the treatment of phenolic acids significantly decreased the relative abundance of Trichoderma,Penicillium,Pseudomonadales,Xanthomonadales,and *** organic acids had a significant negative effect on the relative abundance of Pseudomonadales and Streptomycetales,which led to significantly increased abundance of Fusarium,Xanthomonadales,Micrococcales,and Gemmatim *** based on the non-invasive micro-test techniqueindicated that the root exudates increased the H+ efflux and plasma membrane H+-ATPase activity in the pathogenic fungi and decreased them in the beneficial *** created an acidic environment for the inhibition of beneficial bacteria and accumulation of specialized plant *** study explains the mechanisms behind microflora shift and structure disorder caused by root exudates in a continuously *** rhizosphere soil through chemical language.

关键词： Plant-microbe interactions Replanting disease Rhizosphericdialogue Allelopathic Consecutive monoculture

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Degradation potential and preliminary metabolic pathway analysis of 17β-estradiol (E2) by Pseudomonas putida M39

Degradation potential and preliminary metabolic pathway anal...

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第二十次全国环境微生物学学术研讨会

作者： Ma Lin Yang Qi Amit Pratush Wu Yi-Rui Huang Tong-Wang Hu Zhong Department of Biology College of Science Shantou University Shantou China

In present study M39 a potential E2 degrader was isolated from deep sea water (depth 208 meter) from South China *** both microbiological and molecular characterization of isolated strain revealed that the isolated bacterial strain was bacillus in shape belongs to genus Pseudomonas,and class *** strain was gram negative in *** optimization of growth media and culture condition results of the M39 strain showed that it exhibited maximum growth and E2 degradation at 25℃C,pH 6 and 3% *** was seen that M39 bacterial cells metabolize E2 best (51%) when gown in the presence of sole carbon sources for 9 *** degradation potential analysis was carried out by *** the E2 metabolism by M39 a new intermediate compound (COA-E1) was observed first time after 6 h of *** compound was not accumulated during the reaction because there was no such compound found in later hrs of same *** presence of this new intermediate compound was confirmed by GC-MS *** study only showed the preliminary results about the finding out the new intermediate compound during E2 transformation to E1.

关键词： Pseudomonas putida M39 17β-E2 Metabolic intermediates South China Sea COA-E1

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Biosynthesisand characterizationofselenium nanoparticles mediated by fungus Mariannaea ***

Biosynthesisand characterizationofselenium nanoparticles med...

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第二十次全国环境微生物学学术研讨会

作者： Xiaofang Pei Ying Yang Liyu Ran Xin Shi XinTian Mingrui Li Bo Zhao YuanyuanQu State Key Laboratory of Fine Chemicals Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education) School of Environmental Science and Technology Dalian University of Technology Dalian 116024 China

The biosynthesis of selenium nanoparticles (SeNPs) by *** was reported for the first *** cultural conditions,including SeO2 concentrations and pH were studied,2 mM SeO2 and pH 10 were favorable for the removal of Se(Ⅳ),and extracellular SeNPs were observed in alkaline ***-vis spectroscopy,transmission electron microscopy (TEM),scanning electron microscope (SEM),X-ray diffraction (XRD),Fourier transform infrared spectroscopy (FTIR) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS) were used to characterize *** results showed that removal of Se(Ⅳ) ions occurred during the late exponential phase of strain *** and SEM images revealed that spherical nanoparticles were deposited on the cell war and in the cytoplasmic region of strain HJ with a good size *** pattern suggested the standard hexagonal phase of selenium ***,XPS and selective passivation experiments implicated that carboxyl and amide groups were involved in the formation process and Se(Ⅰ) or Se(Ⅱ) species were demonstrated to be *** on the analyses of SDS,the upregulated proteins of MW ~91.6 and ~37.4-57.2kDa might promote SeNP formation,and a number of proteins adsorbed on the surface of nanoparticles,which may act as a natural stabilizing agent.

关键词： Selenium nanoparticles Mariannaea sp. Biosynthesis Characterization Mechanism

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一体化厌氧氨氧化颗粒污泥的优化培养与应用研究

一体化厌氧氨氧化颗粒污泥的优化培养与应用研究

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第二十次全国环境微生物学学术研讨会

作者：李津青刘红辉逯敏刘新春中国科学院大学资源与环境学院北京100049

厌氧氨氧化(anaerobic ammonium oxidation,Anammox)是20世纪80年代末期新发现的一种氮的自养转化途径,指在厌氧条件下,厌氧氨氧化微生物以NO2--N为电子受体,氧化NH4+-N为氮气的生物过程,厌氧氨氧化反应每消耗1个单位的氨氮和1.32个单... 详细信息

厌氧氨氧化(anaerobic ammonium oxidation,Anammox)是20世纪80年代末期新发现的一种氮的自养转化途径,指在厌氧条件下,厌氧氨氧化微生物以NO2--N为电子受体,氧化NH4+-N为氮气的生物过程,厌氧氨氧化反应每消耗1个单位的氨氮和1.32个单位的亚硝氮,便会生成0.26个单位的硝态氮,该过程与传统生物脱氮工艺相比不需有机碳源、脱氮效率高、能耗与成本低等特点.但厌氧氨氧化菌生长速率缓慢,倍增时间长,导致目前厌氧氨氧化反应器普遍存在启动缓慢的问题,荷兰鹿特丹DokHaven污水厂的ANAMMOX反应器工程,启动时间长达3.5年.奥地利Strass水厂的DEMON工艺启动时间为0.5年.北京高碑店污水厂厌氧氨氧化中试启动时间为0.5～1年,主要是由于接种的普通活性污泥中厌氧氨氧化菌丰度较低.颗粒污泥系统因其具有良好的沉淀性能,可缩短反应器的启动时间,受到人们的关注,同时采用颗粒污泥工艺有助于提高反应器对微生物的持流能力,保证反应器的稳定运行.本实验采用两种不同类型的反应器:改良UASB反应器和SBR反应器,培养厌氧氨氧化颗粒污泥;反应器的启动过程,分别通过控制HRT和沉淀时间,快速淘洗出反应器内的絮体,以快速实现污泥的颗粒化,驯化功能菌.研究了不同粒径颗粒污泥(0.2-0.5mm、0.5-1mm和＞1mm)的脱氮特性、不同C/N比及实际废水热水解消化液对系统长期稳定运行的影响,以期缩短厌氧氨氧化反应器的启动时间,为厌氧氨氧化工艺的工程化应用提供技术支持.同时利用实时定量PCR和高通量测序等分子生物学技术对反应器运行过程中絮体污泥及颗粒污泥中的微生物群落进行分析,探究厌氧氨氧化工艺的脱氮机制和微生物响应变化规律.实验结果表明:不同粒径颗粒污泥(0.2-0.5mm、0.5-1mm和＞1mm)的脱氮特性差异较大,0.2-0.5mm、0.5-1mm、＞1mm颗粒的AOB和NOB活性依次降低,厌氧氨氧化活性随着粒径的增加而升高,高通量结果显示0.2-0.5mm、0.5-1mm、＞1mm颗粒中的厌氧氨氧化菌是Candidatus_Kuenenia,其中0.2-0.5mm占0.11％、0.5-1mm占3.06％、＞1mm颗粒占17.50％;在C/N比提高过程中(由1到1.5、2、3),颗粒污泥形态发生明显变化,镜检显示中间红色部分比例逐渐减少,外围出现黄色包围圈,有向好氧颗粒污泥转化的趋势,总氮去除负荷由0.59kg/(m3·d)降到0.34kg/(m3·d),平均总氮去除率先由94.75％升到95.07％后降到87.55％,结果表明CANON工艺的C/N比不宜超过1.5;热水解消化液的加入会引起反应器总氮去除负荷的明显降低,大颗粒污泥严重破碎,可能与进水COD浓度有关.

关键词：污水处理厌氧氨氧化颗粒污泥微生物群落持流能力脱氮特性

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Biosynthesis of gold nanoparticles using fungus Trichoderma ***-Go and their catalysis in reduction of 4-nitrophenol and decoloration of azo dyes

Biosynthesis of gold nanoparticles using fungus Trichoderma ...

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第二十次全国环境微生物学学术研讨会

作者： Xuanying Li Hao Feng Ziyi Ju Jian Hui Lu Hangzhou Lu Yuanyuan Qu Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education) School of Environmental Science and Technology Dalian University of TechnologyDalian 116024 China

Biological synthesis of gold nanoparticles (AuNPs) has attracted more and more attention due to its advantages of mild conditions,low cost and environment-friendly *** the present study,biosynthesis of gold nanoparticles (AuNPs) by the cell-free extracts of a fungus Trichoderma ***-Go was *** as-synthesized particles were characterized by UV-vis spectroscopy,transmission electron microscopy (TEM).The effects of different parameters on AuNPs biosynthesis were investigated and cell-free extract concentration of 90 mg/L and reacting for 7 days were demonstrated to be suitable for AuNPs *** AuNPs were sphere shapes,with size range of 1-24 nm and an average size of 9.8 *** as-synthesized AuNPs were demonstrated to have good catalytic activities for the reduction of nitro-aromatics (2-Nitrophenol,3-Nitrophenol,4-Nitrophenol,2-Nitroaniline,3-Nitroaniline) with the catalytic rate constants of 7.4×10-3 s-1,10.3×10-3 s-1,4.9×10-3 s-1,5.8×10-3 s-1,15.0×10-3 s-1,***,in decolorization of azo dyes,the AuNPs showed excellent catalytic performance with the decolorization rate of 82.2%-97.5%.The assynthesized AuNPs with small diameter exhibited great potentiality in catalysis and decolorization,which giving AuNPs much value of application.

关键词： Gold nanoparticles Biosynthesis Trichoderma sp. Catalytic activity

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Biosynthesis of indigo by Cupriavidus *** andits functional gene

Biosynthesis of indigo by Cupriavidus *** andits functional ...

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第二十次全国环境微生物学学术研讨会

作者： Shengnan You Yuxin Lin Duandi Zhou Shiqin Long Qi Li Rouyi Wang Yuanyuan Qu Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education) School of Environmental Science and Technology Dalian University of Technology Dalian 116024 China

The biosynthesis of indigo has been attracting a huge attention for its increasing *** this study,a novel bacterial strain IDO capable of utilizing indole as sole carbon source was isolated from soil samples and identified as Cupriavidus *** to 16S rRNA sequence *** found that Cupriavidus *** IDO was able to degrade 100 mg/L indole in 12 *** performance liquid chromatographymass spectrometry analysis indicated that indigo was the major products generated during indole *** genomics,proteomics and functional studies were applied to investigate the genetic determinants for indigo biosynthesis in strain Cupriavidus ***.A total of 317 proteins were notably altered (262 up-and 55 downregulated) in the cells growing in indole mineral salt medium with respect to that in sodium citrate *** the related indole oxygenase genes were knocked out,strain IDO was unable to degrade indole and produce ***,the indole oxygenase genes were cloned from strain IDO and successfully expressed in Escherichia coli BL21 (DE3) (designated as ING_IDO).Strain ING_IDO was able to produce 55 mg/L indigo from tryptophan *** optimization of induction time,IPTG concentration and culture medium,indigo production was increased to 110 mg/*** study obtained valuable indole oxygenase and offered the promise of applying strain ING IDO for producing indigo.

关键词： Biotransformation Cupriavidus sp. indole oxygenase metabolic pathway indigo

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Distinct rhizosphere effecton active and total microbial communities in paddy soils

Distinct rhizosphere effecton active and total microbial com...

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第二十次全国环境微生物学学术研讨会

作者： Hu Li Jian-Qiang Su Xiao-Ru Yang Bo-Sen Weng Yong-Guan Zhu Key Lab of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Key Lab of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences

Rhizosphere microbes are critical for plant health and biogeochemical *** the diversity of active microorganisms in the rhizosphere is key to enhancing plant growth and *** examined rhizosphere microbial communities of rice by comparison of the 16S ribosomal subunit amplicons generated from both total (DNA-based,16S rDNA) and active (RNA-based,16S rRNA) soil *** based on 16S rDNA showed high diversity,but with little change in microbial populations across growth *** of 16S rRNA recovered much less diversity,demonstrating that much of the 16S signal was derived from free DNA,dead or inactive *** rRNA analysis showed a stable microbial population present in the rhizosphere,and this was distinct from that in the bulk soil,which was also stable across the growth *** exudates (e.g.,acetate,lactate,oxalate and succinate),as one of major components contributing to rhizosphere effect,appeared to shape the microbial community,with some taxa (e.g.,Oxobacter,Lanchnospiraceae,Coprococcus and α-Proteobacteria) being enhanced in the *** compartment (rhizosphere vs bulk) had a greater effect on microbial communities than did plant phenological stages,especially at the rRNA *** results suggested that rhizosphere effect play key roles in structuring microbial communities in rhizosphere soils with distinct effect on active and total microbial communities.

关键词： rice rhizosphere bacterial community phenological stages RNA DNA root exudates

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Revealing the relationship between the biodiversity and performance in microbial electrolysis cells operated with the diversity gradient

Revealing the relationship between the biodiversity and perf...

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第二十次全国环境微生物学学术研讨会

作者： Zhaojing Zhang Ye Deng Yuanyuan Qu State Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education Chi Key Laboratory of Environmental Biotechnology Research Center for Eco-Environmental Science Chines State Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education Chi

Understanding the relationship between the biodiversity of microbial communities and ecosystem functioning has been one of the grand challenges among ***,the extremely diverse of natural microbial communities makes it impossible to completely *** electrolysis cells (MEC) especially the anodic biofilm community is an ideal model to explore this relationship within microbial ecosystem due to its moderate complexity,easy controllability and measurable *** this study,we constructed a series of biofilm communities with different biodiversity by diluting the input source community,and applied the high-throughput sequencing of 16S rRNA genes to assess the diversity of anodic microbial communities in *** performances of MEC reactors were monitored,and the high-throughput sequencing of 16S rRNA genes were applied to assess the structures and diversity of microbial communities in MEC *** results demonstrated that biofilms communitieswith a relatively higher α-diversity could obtain a better performance of MEC ***,null model analysis indicated that both deterministic and stochastic processes played important roles in controlling the assembly of the anodic biofilm communities in MECs,but the relative importance of the stochastic processes were decreased substantially with the increasing dilution *** results presented in this study represent important contributions to the understanding of the mechanisms involved in shaping microbial biodiversity,and provide new insights into our understanding of the relationship between diversity and ecosystem functioning.

关键词： Diversity gradient Microbial community assembly Microbial electrolysis cell Biodiversity

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