检索结果-内蒙古大学图书馆

您好，读者！请登录

内蒙古大学图书馆

首页
概况
党建
资源
服务
科研支持
- 论文收录引用证明
- 科技查新
知识产权
档案馆
帮助

咨询与建议

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

您的常用邮箱：*

您的手机号码：*

问题描述：

当前已输入0个字，您还可以输入200个字

全部搜索
期刊论文
图书
学位论文
标准
纸本馆藏
外文资源发现
数据库导航
超星发现

高级检索

时间限定

出版年份：

文献类型

图书期刊文献学位论文多媒体

馆藏选择

电子馆藏纸本馆藏

核心期刊

全部期刊 SCI 收录期刊 SSCI 收录期刊 EI 收录期刊 CSCD 收录期刊 CSSCI 收录期刊

语言

中文英文

文献类型

期刊文献图书学位论文标准纸本馆藏

帮助

文字说明：

T=题名（书名、题名），A=作者（责任者），K=主题词，P=出版物名称，PU=出版社名称，O=机构（作者单位、学位授予单位、专利申请人），L=中图分类号，C=学科分类号，U=全部字段，Y=年（出版发行年、学位年度、标准发布年）

检索规则说明：

AND代表“并且”；OR代表“或者”；NOT代表“不包含”；(注意必须大写,运算符两边需空一格)

检索范例：

范例一：(K=图书馆学 OR K=情报学) AND A=范并思 AND Y=1982-2016
范例二：P=计算机应用与软件 AND (U=C++ OR U=Basic) NOT K=Visual AND Y=2011-2016

分类表

所选分类

>> <<

限定检索结果

文献类型

482 篇 会议
2 篇 期刊文献

馆藏范围

484 篇 电子文献
0 种 纸本馆藏

日期分布

学科分类号

57 篇 理学
- 32 篇 生态学
- 24 篇 生物学
- 1 篇 大气科学
47 篇 工学
- 25 篇 环境科学与工程（可...
- 7 篇 农业工程
- 6 篇 水利工程
- 3 篇 冶金工程
- 3 篇 化学工程与技术
- 2 篇 材料科学与工程（可...
- 1 篇 电气工程
- 1 篇 公安技术
42 篇 农学
- 33 篇 农业资源与环境
- 3 篇 作物学
- 3 篇 兽医学
- 2 篇 水产
- 1 篇 植物保护
26 篇 医学
- 21 篇 药学(可授医学、理...
- 3 篇 中药学(可授医学、...
- 2 篇 基础医学(可授医学...
- 1 篇 公共卫生与预防医...
6 篇 管理学
- 6 篇 公共管理

主题

20 篇 微生物群落
18 篇 生物降解
15 篇 微生物
14 篇 高通量测序
12 篇 生物修复
10 篇 微生物多样性
10 篇 群落结构
9 篇 多环芳烃
9 篇 厌氧消化
9 篇 多样性
8 篇 降解机理
7 篇 细菌
7 篇 代谢组学
7 篇 红树林
6 篇 胞外多聚物
6 篇 基因组学
5 篇 抗生素抗性基因
5 篇 厌氧氨氧化
5 篇 群体感应
5 篇 转录组学

机构

25 篇 浙江大学
16 篇 厦门大学
14 篇 中国科学院大学
12 篇 四川大学
10 篇 南京农业大学
10 篇 天津城建大学
10 篇 上海交通大学
10 篇 浙江工业大学
9 篇 汕头大学
8 篇 黑龙江八一农垦大...
7 篇 农业生物技术国家...
7 篇 贵州师范大学
7 篇 宁波大学
7 篇 武汉大学
7 篇 华中农业大学
6 篇 中国科学院微生物...
6 篇 山东大学
6 篇 哈尔滨工业大学
6 篇 中国科学院城市环...
6 篇 浙江省水体污染控...

作者

11 篇 汤岳琴
10 篇 郭建博
10 篇 逯彩彩
9 篇 胡忠
9 篇 宋圆圆
9 篇 李海波
9 篇 韩懿
9 篇 郑伟
9 篇 袁红莉
9 篇 杨金水
8 篇 王伟东
8 篇 徐向阳
7 篇 翁庆北
7 篇 meiying xu
6 篇 田蕴
6 篇 吴庆珊
6 篇 郭峰
6 篇 杨毅
6 篇 苟敏
6 篇 孙照勇

语言

383 篇 中文
101 篇 英文

检索条件"任意字段=第二十次全国环境微生物学学术研讨会"

共 484 条记录，以下是461-470 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

Effect of C/N ratio on nitrogen removal by anammox coupling denitrification process and microbial communities in the anaerobic baffled reactor (ABR)

Effect of C/N ratio on nitrogen removal by anammox coupling ...

引用

第二十次全国环境微生物学学术研讨会

作者： Chongjun Chen Xuliang Yu Yaoqi Wang Min Zhang Ying Jiang Jianfang Wang Yaoliang Shen School of Environmental Science and Engineering Suzhou University of Science and Technology Suzhou School of Environmental Science and Engineering Suzhou University of Science and Technology Suzhou

Anaerobic ammonium oxidation (anammox),a lithoautotrophic biological conversion process to remove nitrogen by oxidizing ammonium to N2 using nitrite as the electron acceptor and use CO2 as carbon source under the anoxic ***,the presence of organic carbon source would negatively affect anammox *** could be attributed to the competition for nitrite between anammox bacteria and heterotrophic *** anaerobic baffled reactor (ABR) was initially developed with the partial separation of different bacteria and form a distinct biological phase in each compartment to reduce the ineffective of COD *** removal with different C/N ratio effect was investigated using an ABR anammox reactor with five *** indicated that the ABR reactor could remove nitrogen excellently by anammox coupling denitrification,while the C/N (NO2--N) ratio exert an important effect on nitrogen *** the C/N ratio was 1.29,the ABR could achieve the highest total nitrogen (TN) removal efficiency of 99.9%.Most of TN was removed in the 1st and 2nd compartment,accounted for about 81.0%-97.6% of total TN *** on the nitrogen and carbon balance,the nitrogen removal was result of interaction by anammox coupling denitrification *** the nitrogen removal contribution of anammox was varied from 6.8% to 32.4%.High-throughput Miseq sequencing analyses revealed that the C/N ratio was the most important factor regulating the bacterial community structure,and the predominant phylum changed from Proteobacteria to Chloroflexi with the elevated C/N *** addition,the Candidatus Brocadia was the major anammox bacteria and the percent was varied from 1.0-2.9% to 2.8-9.1%.

关键词： C/N ratio anaerobic baffled reactor (ABR) anammox High-throughput Miseq sequencing microbial communities

来源：评论

学校读者我要写书评

暂无评论

Combination of a Recombinant Bacterium with Organonitrile-degrading and Biofilm-forming Capability and a Positively Charged Carrier for Organonitriles Removal

Combination of a Recombinant Bacterium with Organonitrile-de...

引用

第二十次全国环境微生物学学术研讨会

作者： Ning Hou Zhenlei Yue Yueling Sun Mingyan Huang Hailan Wang Xuejiao An Hailian Zang Dapeng Li Chunyan Li College of Resources and Environment Northeast Agricultural University Harbin 150030Heilongjiang PR China

The immobilization of organonitrile-degrading bacteria via the addition of biofilm-forming bacteria represents a promising technology for organonitrile-containing wastewater treatment,but simple mixing may reduce treatment efficiency,and the adherence of biofilm-forming bacteria is affected by electrostatic *** nitrile hydratase and amidase genes,which play critical roles in organonitriles degradation,were cloned and transformed into the biofilm-forming bacterium Bacillus subtilis N4 to construct the recombinant bacterium *** N4/pHTnha-ami,which possessed organonitrile-degrading and biofilm-forming *** nitrile hydratase and amidase genes were expressed upon lactose *** successfully modified carriers with a positive charge,identified by Fourier Transform Infrared (FI-RT) and X-ray photoelectron spectroscopy (XPS),promoted the adherence of *** N4/pHTnha-ami and biofilms formation (P < 0.05).Biofilms formed by the combination of *** N4/pHTnha-ami and this positively charged carriers demonstrated substantial resistance to organonitriles loading shock,and degraded the initial CN concentration (392.6mg/L) within 24 h in a moving bed biofilm reactor (MBBR) after operation for 32 *** imputed quorum-sensing signal composition of biofilm samples from modified carriers indicated that *** N4/pHTnha-ami was successfully immobilized in the *** detection via high-throughput sequencing confirmed that *** N4/pHTnha-ami became the dominant *** knowledge obtained from this study provides important guidance to enhance the biological oxidation of toxic pollutants in wastewater.

关键词： Organonitrile-containing Wastewater Recombinant Bacterium Nitrile hydratase/amidase pathway Amine-functionalized carrier Organonitrile-degrading and biofilm-forming capability

来源：评论

学校读者我要写书评

暂无评论

鄱阳湖湿地苔草水下分解动态研究

鄱阳湖湿地苔草水下分解动态研究

引用

第二十次全国环境微生物学学术研讨会

作者：马燕天高敏蔡润发何世耀刘亚军吴兰葛刚南昌大学生命科学学院鄱阳湖环境与资源利用教育部重点实验室江西南昌330031

淡水湖泊湿地对于维持湖泊生态平衡、保障周边淡水安全和调节区域气候都有重要的作用.鄱阳湖作为我国最大的淡水湖泊,孕育了广阔的湖泊湿地.肥沃的湿地土壤为湿生植被提供了营养保证,而植物死亡后通过分解过程将大量营养归还湿地.受到... 详细信息

淡水湖泊湿地对于维持湖泊生态平衡、保障周边淡水安全和调节区域气候都有重要的作用.鄱阳湖作为我国最大的淡水湖泊,孕育了广阔的湖泊湿地.肥沃的湿地土壤为湿生植被提供了营养保证,而植物死亡后通过分解过程将大量营养归还湿地.受到鄱阳湖季节性水位涨落的影响,湿地中的湿生植被在一年中要经历两个生长周期,分别为每年的3-5月和9-12月,而5-9月为鄱阳湖丰水期,大量植物水淹后死亡分解.湿地作为全球重要的碳汇,储藏了大量的有机碳.而随着全球气候变化的影响,许多湖泊湿地的水文状况发生了巨大变化,湖泊湿地是否能够继续维持碳汇的功能是人们关注的重点.为了详细研究鄱阳湖湿地植被水下分解的动态,采集了鄱阳湖最为典型的植被-苔草作为研究对象,采用网袋分解法原位模拟苔草的水下分解过程以及主要营养元素的释放状况.实验中分别以新鲜样品和烘干样品模拟活体植物淹水分解和自然枯死植物淹水分解的过程.研究结果表明,新鲜苔草和烘干苔草样品水下分解过程中质量损失在前45天最快,质量残余率分别为55.0％和49.5％;经过245天的分解过程之后,质量残余率分别为20.8％和14.3％.新鲜苔草分解速率(k=0.0849/d)明显快于烘干苔草(k=0.0818/d).在分解进行70天时,植物质量残余率有一个明显的升高现象,同时有机质含量(AFDM,ash free dry mass)的迅速降低,可能与植物对周围土壤的吸附有关.两种样品中有机碳TOC的含量在前30天缓慢增加,之后快速降低.所有样品总氮含量TN比较稳定,总磷含量TP在前45天比较稳定,之后迅速升高.实验同时测定了植物残体中包括β-葡萄糖苷酶(Bglu)、β-木糖苷酶(Bxyl)、N-乙酰氨基葡萄糖苷酶(NAG)、磷酸酶(Phos)、酚氧化酶(Phox)和过氧化氢酶(Pero)在内的六种酶活性.结果表明烘干样品中β-葡萄糖苷酶、β-木糖苷酶和磷酸酶活性高于新鲜样品;两种样品在分解前期N-乙酰氨基葡萄糖苷酶和过氧化氢酶活性相似(＜70d),但在后期烘干样品明显高于新鲜样品.新鲜样品中酚氧化酶活性明显高于烘干样品.以上研究结果表明,活体植物在经历水淹死亡后的分解过程明显快于干枯植物,并且在此过程中微生物对于植物分解的贡献降低.鄱阳湖水位变化将会严重影响湖泊湿地中的营养元素循环,并对气候变化产生影响.本研究揭示了鄱阳湖独特的植物水下分解动态,将为湖泊湿地的科学管理提供科学指导.

关键词：湖泊湿地生态系统苔草水下分解营养元素释放规律

来源：评论

学校读者我要写书评

暂无评论

内生真菌枫香拟茎点霉对多环芳烃污染物菲降解能力和机制的研究

内生真菌枫香拟茎点霉对多环芳烃污染物菲降解能力和机制的研究

引用

第二十次全国环境微生物学学术研讨会

作者：傅婉秋徐漫孙凯胡丽燕曹伟戴传超贾永南京师范大学生命科学学院江苏省南京市栖霞区文苑路1号210023

多环芳烃(Polycyclic aromatic hydrocarbons,PAHs)污染物因其致畸、致癌、致突变及高毒性受到广泛关注,菲作为一类具有三个稠和苯环的典型低分子量多环芳烃在环境中的污染程度高.近年来,许多研究者致力于寻求降解环境中PAHs的优化方法... 详细信息

多环芳烃(Polycyclic aromatic hydrocarbons,PAHs)污染物因其致畸、致癌、致突变及高毒性受到广泛关注,菲作为一类具有三个稠和苯环的典型低分子量多环芳烃在环境中的污染程度高.近年来,许多研究者致力于寻求降解环境中PAHs的优化方法以应对这一问题.本课题组前期研究表明,一株广谱内生真菌枫香拟茎点霉Phomopsisliquidambari在体外纯培养条件下具有降解菲的能力,同时已证明该菌株能够与水稻建立共生关系且对环境中的菲污染有去除效果.但到目前为止,关于***对菲的降解机制还知之甚少.因此,利用体外纯培养探究了***降解菲的途径、酶活和基因,同时将***与水稻建立联合体系进行室内蛭石条件下去除菲的研究.我们的结果表明,该菌株能够以50mg L-1菲作为唯一碳源和能源进行生长,并且10天后对菲的降解率超过77％.利用HPLC-MS鉴定出代谢中间产物并提出两条可能的降解途径:菲首先在P450单加氧酶的催化下形成9,10-环氧化菲,之后一条可能的途径是9,10-环氧化菲经环氧化物水解酶的水解作用形成反式9,10-二氢二醇菲,接着在脱氢酶作用下生成9,10-二醇菲,再经邻苯二酚2,3-双加氧酶为9,10-二醇菲加上两个氧原子形成9,10-菲醌.另一条可能的途径是9,10-环氧化菲先在多种酶的作用下转化为9-羟基菲,接着被氧化为9,10-菲醌.两种途径转化成的9,10-菲醌进一步裂解并氧化形成2,2'-联苯二甲酸,最终进入到TCA循环.***中编码三种降解酶(P450单加氧酶、环氧化物水解酶和脱氢酶)的基因(分别为P450SMOB3,EHB3和DHB3)的表达水平在降解过程中也受到调控,其转录水平比内参基因最多分别高出8.99倍、39.88倍和11.62倍.在体内降解方面,***与水稻建立联合体系后可有效去除水稻幼苗体内积累的菲,在水稻培养30天后,建立联合体系的处理组中菲的去除率比未接菌处理高出25.68％.我们的研究表明,***不仅能够应答体外培养时的菲胁迫,也能对积累了菲的植物起到缓解毒性的作用.同时,本研究也将为内生真菌应用于植物体内外环境中的PAHs生物修复奠定基础.

关键词：枫香拟茎点霉多环芳烃污染物降解机理环氧化物水解酶

来源：评论

学校读者我要写书评

暂无评论

重组抗菌肽PSI乳酸克鲁维酵母的高密度发酵及产物的分离纯化研究

重组抗菌肽PSI乳酸克鲁维酵母的高密度发酵及产物的分离纯化研究

引用

第二十次全国环境微生物学学术研讨会

作者：翟立翔李国丽李师翁陈熙明甘肃省极端环境微生物资源与工程重点实验室/中国科学院西北生态环境资源研究院沙漠与沙漠化重点实验室甘肃兰州730000

乳酸克鲁维酵母(Kluyveromyces lactis)是一种能高效表达外源蛋白的酵母表达系统,作为美国FDA认证的食品安全级酵母,具有遗传稳定、蛋白可翻译后加工、产物可分泌到胞外、可高密度发酵且发酵过程无产气现象,不会发生爆罐、发酵过程中所... 详细信息

乳酸克鲁维酵母(Kluyveromyces lactis)是一种能高效表达外源蛋白的酵母表达系统,作为美国FDA认证的食品安全级酵母,具有遗传稳定、蛋白可翻译后加工、产物可分泌到胞外、可高密度发酵且发酵过程无产气现象,不会发生爆罐、发酵过程中所需的诱导剂是乳糖或半乳糖,相对甲醇诱导更安全、表达产物可直接应用于食品及饲料工业中等优点.本研究成功构建了重组抗菌肽PSI的乳酸克鲁维酵母基因工程菌,在摇瓶培养的基础上,建立了100L发酵罐中高密度发酵的工艺及产物分离纯化方法.工程菌株在100 L发酵罐中发酵24 h后开始流加葡萄糖使菌体密度增加,当酵母生长进入稳定期,菌体湿重达到190 g/L,开始流加乳糖诱导,诱导25 h后结束发酵,菌体湿重达280 g/L,发酵液中抗菌肽效价达2.36×109 U/L,实现了100 L发酵罐高密度发酵生产抗菌肽.SDS-PAGE结果表明：随着诱导时间的增加,发酵液上清中蛋白表达量逐渐增加,到第25h,表达产物量最多.用YDFM、BSM、YDFMY和BSMY四种培养基进行发酵的抗菌肽效价分别为1.22× 109 U/L、2.36× 109 U/L、2.01×109 U/L和2.78× 109 U/L,表明BSM诱导培养基更适于重组工程菌表达抗菌肽,且更为经济和高效.为了确定乳糖是否可作为半乳糖的替代品,将一定量的乳糖和半乳糖分别作为诱导物诱导酵母表达抗菌肽PSI,在不同诱导物下发酵液中抗菌肽效价分别为2.36× 109 U/L和3.28× 109 U/L,表明乳糖也可作为诱导物诱导酵母表达抗菌肽.对发酵工艺进行了优化,用BSM培养基发酵培养至菌体密度达到190 g/L时开始添加乳糖诱导,乳糖添加量为5 kg,补料流加速度为1 L/h,诱导25 h,菌体细胞湿重达280 g/L,发酵液中蛋白产量达27.8 mg/L,抗菌肽PSI效价达到2.36×109 U/L.采用多种分离纯化方法对发酵液中抗菌肽PSI进行分离纯化,结果表明阴离子交换色谱纯化效率最高,纯化后蛋白效价为8.83× 109 U/L,其次为丙酮沉淀、超滤、硫酸铵分级沉淀,纯化后蛋白效价依次为6.35× 109、4.56×109、3.28× 109 U/L.

关键词：抗菌肽PSI 乳酸克鲁维酵母基因工程菌高密度发酵分离纯化

来源：评论

学校读者我要写书评

暂无评论

A DNA extraction method targeting improved uniformity and representativeness for compost bacterial community profiling

A DNA extraction method targeting improved uniformity and re...

引用

第二十次全国环境微生物学学术研讨会

作者： Wenxia Wang Yuting Liu Zhanjun Wang Huimin Jiang Jianqiang Xu Hongyu Xue Weiping Xu Department of Environmental and Ecological Engineering School of Food and EnvironmentDalian Univer Panjin Industrial Technology Institute of Dalian University of Technology 124221 PanjinChina Scho School of life science and medicine Dalian University of Technology Panjin campus 124221Panjin Department of Environmental and Ecological Engineering School of Food and EnvironmentDalian Univer

Microbial community profiling method has been improved substantially in the past three decades,from the electrophoresis dependent Ribosomal Intergenic Spacer analysis (RISA) and Denaturing Gradient Gel Electrophoresis (DGGE) analysis,to the electrophoresis independent Next Generation Sequencing (NGS),for ***,no matter how advanced the profiling method is,a DNA extraction method with high uniformity and representativeness of the microbial community samples is still the fundamental key to guarantee a preciseness analysis.A novel DNA extraction method (FC) was developed,using filter paper pre-absorbance treatment to magnify extraction material,and Chelex resin to absorb DNA contaminants,aimed to improve the uniformity and representativeness of the extracted *** developed FC method was compared with commercial kit method using fresh compost material (K) and kit method using filter paper pre-absorbance material (FK) as *** was tested by Bacteria-RISA (B-RISA) analysis to profile bacterial community *** BRISA bands were interpreted into fragment length,transferred to a data matrix,clustered and analyzed by Multivariate regression tree (MRT) method to interpret the interaction between composting days/chemical properties and bacterial community *** B-RISA gel results showed that the FC method had higher similarity of bacteria bands among triplicates,while K method showed less similarity among *** is consistence with the clustering analyses,that FC samples clustered according to the time and replicates,while K samples clustered with no *** FC,FK and K gels,the FC and FK method showed higher similarity than the K method,whereas the FK and K method showed less *** suggested that the difference in between FC and K method was not because of the limitation of FC method,but because of the difference in the extraction *** limited material for DNA extraction,as in the commercial kit,may cause

关键词： DNA extraction compost microbial community profiling uniformity representativeness

来源：评论

学校读者我要写书评

暂无评论

Contribution of acetogenesis to anaerobic degradation in methanogenic rice field soils

Contribution of acetogenesis to anaerobic degradation in met...

引用

第二十次全国环境微生物学学术研讨会

作者： Bo Fu Martin Blaser Ralf Conrad School of Environmental and Civil Engineering Jiangsu Key Laboratory of Anaerobic Biotechnology Ji Department of Biogeochemistry Max Planck Institute for Terrestrial MicrobiologyMarburg Germany

Acetate is an important intermediate in the anaerobic degradation of organic *** is not only produced by fermentation but also by the reduction of CO2 via the acetyl-CoA pathway (acetogenesis).However,the interplay of this process in methanogenic rice field soils is not fully *** acetogenesis results in a rather strong depletion of the 13 C of ***,we measured the δ 13 C of acetate,CO2 and CH4 that were produced during methanogenic degradation of organic matter in rice paddy soils from two geographical origins (Philippines and Italy) and used three different strategies to estimate the contribution of acetogenesis to acetate formation: (1) Incubation of soil slurries under elevated concentrations of H2/CO2 to specifically activate the H 2-dependent communities;(2) incubation at three different temperatures (15,30,50℃) to shift the conditions for H2 consumption;(3) incubation in the presence of inhibitors presumed to inhibit acetogenesis (KCN) or methanogenesis (BES).Only incubations under elevated H 2 /CO 2 resulted in 13 C-depleted acetate (δ 13 C of-68 to-65%‰) compared to the control (δ 13 C of-25%o).Temperature and presence of inhibitors also affected the δ 13 C of acetate,CO2 and/or CH4,but δ 13 C of acetate was never as low as after addition H2/CO2.A significant 13 C enrichment of acetate at 15℃ in presence of BES and KCN indicated that H2-dependent acetogenesis is a favoured process at low *** numbers in the Philippine soil of the fhs gene coding for the formyl-tetrahydrofolate synthetase of the acetyl-CoA pathway were on a similar order of magnitude (10 6 per gram dry soil) irrespectively of the different incubation *** results indicate that chemolithotrophic acetogenesis was operative in methanogenic rice soil at 15℃ but was more important at elevated H2 /CO2 concentrations.

关键词： Acetate Acetogenesis Carbon isotope Temperature Rice soil 33

来源：评论

学校读者我要写书评

暂无评论

Exploring Abundance, Diversity and Variation of a Widespread Antibiotic Resistance Gene in Wastewater Treatment Plants

Exploring Abundance, Diversity and Variation of a Widespread...

引用

第二十次全国环境微生物学学术研讨会

作者： Ziyan Wei Kai Feng Shuzhen Li Yu Zhang Hongrui Chen Huaqun Yin Meiying Xu Ye Deng Key Laboratory of Environmental Biotechnology of CAS Research Center for Eco-Environmental Sciences Key Laboratory of Environmental Biotechnology of CAS Research Center for Eco-Environmental Sciences StateKey Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Scienc School of Minerals Processing and Bioengineering Central South University Changsha 410083China State Key Laboratory of Applied Microbiology Southern China Guangdong Institute of Microbiology Gu

An updated sul1 gene sequence database was constructed and new degenerate primers were designed to better investigate the abundance,diversity,and variation of a ubiquitous antibiotic resistance gene,sul1,with PCR-based methods in activated sludge from wastewater treatment plants (WWTPs).Compared to previous sul1 primers,the newly designed degenerate primers showed higher coverage in both in-silico evaluations and activated sludge *** the new primers,the abundance and diversity of sul1 gene,together with 16S rRNA gene,in activated sludge from five WWTPs in summer and winter were determined by quantitative PCR and MiSeq *** sul1 gene was found to be prevalent and displayed a comparable abundance (average 8.06%) to the total bacterial cells across all ***,compared to the significant temporal and geographical divergences in the quantity and diversity of bacterial communities in WWTPs,there were no significant temporal or spatial variations of representative OTUs ofsull gene in most ***,the representative sul1 OTUs showed fairly close phylogeny and there was no obvious correlation between them and the dominant bacterial genera,suggesting that mobile genetic elements likely play a greater role than specific microbial taxafor sul1 gene in WWTPs,and a mobile component containing the sul1 gene undergoes frequent transfer among different bacterial species without any stable hosts.

关键词： 16S rRNA sul1 degenerate primer design quantitative PCR MiSeq sequencing

来源：评论

学校读者我要写书评

暂无评论

Oxidized graphene and polymerase impacted amplication errors during PCR and bacteria community's structure and diversity

Oxidized graphene and polymerase impacted amplication errors...

引用

第二十次全国环境微生物学学术研讨会

作者： Shuzhen Li YuanYuan Qu Ye Deng State Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education Chi State Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education Chi Key Laboratory of Environmental Biotechnology Research Center for Eco-Environmental Science Chines

Graphene oxide (GO) has been demonstrated as an efficient assistant additive to eliminate non-specific amplification of the polymerase chain reaction (PCR).Although many studies have been focusing on exploring its molecular mechanism during PCR process,effect of GO on the reduction of amplication errors,such as chimeric and mismatched sequences,and shaping the composition and diversity of microbial communities have not been systematically explored *** that,KAPA HiFi and Taq DNA polymerase have been widely used in the field of microbial ecology,while KAPA HiFi has much higher fidelity and efficiency but is still prohibitively *** it is worth exploring different kinds of polymerase on the accuracy and specificity of amplification errors and microbial *** this study,we explored the effect of GO and different kinds ofpolymerase (KAPA HiFi and Taq DNA polymerase) on removing amplification errors,such as chimeric and mismatched sequences on two kinds of mock communities (an even and a staggered mock communities) and environmental *** indicated that GO could not effectively reduce mismatches in sequencing procedure and raise chimeric sequences in both mock ***,KAPA HiFi protocol significantly reduced the chimera proportion and mismatched sequences numbers in even *** for the structure and diversity of communities,GO made no contribution on microbial β-diversity but effectively reduced the difference of α-diversity within the community,thus may act as an efficient additive removing non-specific amplification in the PCR process according to this ***,composition of community was almost no difference by using KAPA HiFi or Taq DNA polymerase protocol on even and staggered mock *** results presented in this study represent new insights to the understanding of the effect of GO and polymerases on the detection of chimeric and mismatched sequences,and shaping the composition and diversi

关键词： Oxidized graphene Mismatch Chimera KAPA HiFi Bacteria community

来源：评论

学校读者我要写书评

暂无评论

Effect of air pollution on the total bacteria and pathogenic bacteria in different sizes of particulate matter

Effect of air pollution on the total bacteria and pathogenic...

引用

第二十次全国环境微生物学学术研讨会

作者： Huan Liu Xu Zhang Hao Zhang Xiangwu Yao Meng Zhou Jiaqi Wang Zhanfei He Huihui Zhang Liping Lou Weihua Mao Ping Zheng Baolan Hu Department of Environmental Engineering Zhejiang University Hangzhou 310058 China The Center of Analysis and Measurement Zhejiang University Hangzhou 310058 China Department of Environmental Engineering Zhejiang University Hangzhou 310058 China Research Center

In recent years,airborne particulate matter pollution events have occurred frequently in China during the *** studies have focused on the physical and chemical composition of polluted *** studies have examined the bacterial bioaerosols both indoors and *** few studies focused on the relationship between air pollution and bacteria,especially pathogenic *** PM samples of different diameters and different air quality index (AQI) values were collected from December 2014 to January 2015 in ***-throughput sequencing of 16S rRNA was used to categorize the airborne bacteria according to the family or *** on the NCBI database,the "Human Pathogen Database" which is related to human health was *** all the PM samples,the diversity of total bacteria was low in the moderately or heavily polluted ***,in PM2.5 and PM10 samples,the relative abundance of pathogenic bacteria was the highest in the moderately or heavily polluted *** the PM samples of different particle sizes,the diversity of the total bacteria and the proportion of pathogenic bacteria in the PM10 samples were different from those in the PM2.5 and TSP *** composition of PM samples with different sizes range may be responsible for the *** relative humidity,carbon monoxide and ozone concentrations were the main factors that affected the diversity of total bacteria and the proportion of pathogenic *** the different environmental samples,the compositions of total bacteria in all the airbome PM samples were very similar but were completely different from those in the water,surface soil,and ground dust ***-distance transport of the airflow may influence the composition of the airborne *** study of the pathogenic bacteria in airbome PM samples can provide reference for environmental and public health ***: Different concentrations and sizes of particulate matter and varied enviro

关键词： pathogenic bacteria total bacteria airborne particulate matter air quality index

来源：评论

学校读者我要写书评

暂无评论

没有更多数据了...

全选清除本页清除全部题录导出标记到“检索档案”

共49页 << < 40 41 42 43 44 45 46 47 48 49 > >>

检索报告对象比较合并检索0

隐藏清空

合并搜索

回到顶部

执行限定条件

内容：

评分：

请选择保存的检索档案：

请选择收藏分类：

订阅名称：

通借通还

温馨提示：

图书名称：

借书校区：

取书校区：

手机号码：

邮箱地址：

一卡通帐号：

电话和邮箱必须正确填写，我们会与您联系确认。

联系人：

所在院系：

联系邮箱：

联系电话：

内蒙古自治区呼和浩特市赛罕区大学西街235号邮编: 010021

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：