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Neuroprotective effect of the Compound Danshen tablet in Aβ25-35 -induced spatial memory impairment in mice

Neuroprotective effect of the Compound Danshen tablet in Aβ...

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第二届世界天然药物和传统药物药理学学术会议

作者： Zhen LU~1,Yan TENG~2,Wen WANG~2,Meng-Qi ZHANG~2,Li-Tao LIU~1,Shao-Ju JIN~1, Li-Ming ZHOU~1,Li-Hong WAN~(1*) (1.Department of Pharmacology,West China School of Preclinical and Forensic Medicine,Sichuan University, Chengdu 610041,China 2.Basic Medicine 2009 undergraduate students,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,China)

ADM Compound Danshen tablet（CDT）,a Traditional Chinese Medicine,has recently been reported to improve spatial cognition in a rat model of Alzheimer's ***,in vivo neuroprotective mechanism of the CDT in models of spatial memory impairment is not yet *** present study is aimed to elucidate the cellular mechanism of CDT on Aβ-induced cognitive impairment in *** Mice were randomly divided into 5 groups;the control group（sham operated）,the AD model group,the positive drug group,and large and small dosage of the CDT groups,*** was administered at a dose of 1.62 g·kg and 0.405 g·kg for 3 *** mice in the positive drug group were treated with 0.4 mg·kg of Huperzine A, whereas the mice of the control and AD model groups were administrated orally with equivalent *** 7 d of preventive treatment, mice were subjected to lateral ventricle injection of Aβ to establish the mice model of Alzheimer's *** water maze test was used to measure the capability of spatial *** of TNF-α,IL-6,ChAT,RACK1 and BDNF were analyzed by RT-PCR,immunohistochemistry and *** The results showed that Aβ caused spatial memory impairment as demonstrated by performance in the Morris water maze *** was able to confer a significant improvement in spatial memory,and protect mice from Aβ-induced ***,CDT also inhibited the increase of TNF-α,IL-6 level,and increased the expression of ChAT,RACK1 and BDNF in brain as compared to model *** The findings strongly implicate that CDT may be a useful treatment against learning and memory deficits in mice by rescuing imbalance between cytokines and neurotrophins.

关键词： compound Danshen tablet Aβ25-35 neuroprotective effect RACK1 BDNF

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Protective effect of Zige lyophilized powder preconditioning in an hypoxia/reoxygenation model of HUVECs

Protective effect of Zige lyophilized powder preconditioning...

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第二届世界天然药物和传统药物药理学学术会议

作者： Cong-Cong ZHANG,Jia-Lan LIU,Hui ZHANG,Xiao-Yu XU~* (College of Pharmaceutical Sciences,Southwest University,Chongqing Engineering Research Center for Pharmacodynamics Evaluation,Chongqing 400716,China )

AIM To study whether Zige lyophilized powder may improve survival of vascular endothelial cells（EC） following ischemia/reperfusion injury was investigated in this *** EC cultured from human umbilical vein were sealed in a flowthrough chamber mounted on an inverted microscope and perfused with a constant flow of Krebs *** 1 h of equilibration, HUVEC underwent 4 h of hypoxia（H） and 1 h of reoxygenation（R） to mimic ischemia/reperfusion,addition of Zige lyophilized powder to culture medium at start of *** H/R caused significant viability loss and an release of LDH increase compared to the normoxic control,12.5 mg·L Zige lyophilized powder enhanced cell viability（P<0.05）.Also,49.0,24.5 and 12.5 mg·L Zige lyophilized powder prevented the decline of SOD activity during H/R（P<0.05）.Furthermore,24.5 and 12.5 mg·L Zige lyophilized powder significantly inhibited the increase of MDA and NO ***,apoptotic related protein levels were measured by Western blotting ***-2 protein level increased significantly in the 24.5 and 12.5 mg·L Zige lyophilized powder group. 49.0,24.5 and 12.5 mg·L Zige lyophilized powder significantly decreased Bax level and up-regulated Bcl-2/Bax *** activation and apoptosis were monitored in the reoxygenated cells.49.0 and 24.5 mg·L Zige lyophilized powder decreased caspase 3 expression（P<0.01）.CONCLUSION The results indicate that Zige lyophilized powder prevents damage H/R induced to HUVEC, the cytoprotection of Zige lyophilized powder appears to be associated with the enhancement of the ability anti-oxidation and anti-apoptosis.

关键词： Zige lyophilized powder hypoxia/reoxygenalion injury HUVEC

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Screening of mTOR signal pathway inhibitors

Screening of mTOR signal pathway inhibitors

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第二届世界天然药物和传统药物药理学学术会议

作者： Ju-Ming YAN,Hong-Yu ZHOU,Xing-Yao LI,Yan LI~* (Kunming Institute of Botany,Chinese Academy of Sciences,132 Lanhei Road,Heilongtan, Kunming 650204,China)

AIM The mammalian target of rapamycin（mTOR） signaling pathway has been shown to play important role in regulating cell growth,proliferation and *** signal pathway is over-activated in many cancer cells which results in cancer invasion and metastasis.A number of evidences have demonstrated that mTOR signal transduction pathway is an active target for antitumor drug *** this study,we established a cell-based assay and screened natural compounds inhibiting mTOR signal pathway. METHODS The phosphorylation of 4EBP1 by active mTOR signal regulates the nuclear location of eIF4E,which could be readout of the state of mTOR signaling *** inhibitory effect of compounds was monitored in MEF cells after treatment for 5 h at 10μmol·L concentration,with the rapamycin and PI-103 as the positive ***-to-cytoplasmic eIF4E intensity ratios were collected using high content screening after *** Total 1235 compounds from a natural compound library were screened using the assay and 10 of them turned out to be able to promote the eIF4E nuclear *** verify the inhibitory effect of these positive compounds in cancer cells,we have determined the nuclei-to-cytoplasm eIF4E intensity ratios in six cancer cell lines,SMMC7721,Hep G2,MCF7,MD-MAB-231,A549 and SW480 at 20μmol·L,10μmol·L and 5μmol·L for 5 *** results demonstrated that two of the positive compounds showed markedly inhibitory effect on the mTOR signaling in a dose dependent *** A cell-based assay with the eIF4E nuclear translocation as the indicator of mTOR signaling activation was established and with this assay natural compounds with anti-tumor potential were screened and discovered.

关键词： mTOR signal pathway tumor natural compounds eIF4E nuclear location

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Screening and research selective anti-hepatoma drugs from the traditional Chuang and Yao medicine in Guangxi by tyrosine kinase,angiogenesis as antitumor drug targets

Screening and research selective anti-hepatoma drugs from th...

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第二届世界天然药物和传统药物药理学学术会议

作者： Yue-Wen SUN,Gang LIANG,Jin-Ni WANG (Department of Pharmaceutic School,Guangxi Medical University,Nanning 530021,China)

AIM To screen and research targeting anti-hepatoma drugs from 18 kinds of the traditional Chinese herbal medicines from Yao medicin,Chuang medicine in Guangxi province by antitumor drug target as tyrosine *** 18 Ethanol extracts of Yao medicines,Chuang medicines in Guangxi were obtained by cold-extraction alcohol,then the inhibitory activity of human hepatoma cell line SMMC-7721 with MTT were determined in *** medicated serums with obviously anti-tumor effect of enthanol extracts were prepared according to the serum pharmacology method and further observed its inhibiting effect in same tumor cell line by *** total protein of human hepatoma cell line SMMC-7721 at different time or concentration of extracts was extracted by cell lysis buffer,*** content of tyrosine kinase in total protein of tumor cells was detected by enzyme-linked immunosorbent assay （ELISA）.The model of angiogenesis in allantocheriionof chick embroyo was established so as to observe antiangiogenesis effect of extracts by CAM assay in *** models of xenografted tumor in nude mice with human hepatoma cell line SMMC-7721 were established, and then observated the tumor growth by HE staining and expression of VEGF,CD34 in tumor tissues of each group by immunohistochemistry *** Dysosma versipellis and Hudrocotyle sibthorpioides had stronger anti-tumor ability to human hepatoma cell line SMMC-7721 and BEL-7404 among the screening drugs,the IC of SMMC-7721 was 0.281 and 0.094 g·L;the IC of BEL-7404 was 0.235 and 0.104 g·L,*** results of serum pharmacology showed medicated serum inhibited the growth of human hepatoma cell line SMMC-7721 in varying degrees.10%serum of Dysosma versipellis group had an obvious inhibition effect on tumor compared with 10%serum of sorafenib group,the inhibitory rate was 11.15%.There was significant differences between two groups（P<0.05）.Dysosma versipellis and Hudrocotyle sibthorpioides reduced the level of t

关键词： Chinese herbal medicine herbs anti-hepatoma drugs tyrosine kinase inhibitor angiogenesis VEGF

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Ginsenoside Rg3 attenuates cell migration via inhibition of aquaporin 1 expression in PC-3M cells

Ginsenoside Rg3 attenuates cell migration via inhibition of ...

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第二届世界天然药物和传统药物药理学学术会议

作者： Xue-Jun LI (Department of Pharmacology,School of Basic Medical Sciences,Peking University,Beijing 100191,China) Department of Pharmacology School of Basic Medical SciencesPeking UniversityBeijing 100191China

Ginsenoside Rg3（Rg3）,one of the major effective ingredients in ginseng,has been shown to have various biological effects including anti-cancer *** this study,we detected the anti-migration effect of Rg3 on a high-metastatic prostate cancer cell line,PC-3M,and observed its correlation with AQP1 water channel *** wound healing assay and transwell migration assay,the results showed an inhibitory effects on PC-3M cell migration with the concentration of 1 - 10μmol·L of ***, the AQP1 expression was suppressed by Rg3 on both protein and mRNA *** the AQP1 was overexpressed by pcDNA3- AQP1 plasmid in PC-3M cells,we found that overexpression of AQP1 recovered the cell migration of PC-3M cells,which antagonized Rg3's inhibitory *** the other hand,after silencing the AQP1 expression by tranfected shRNA of AQPl to PC-3M,the cell migration was decreased,similar to the effect of Rg3 *** cell migration was inhibited lower than baseline *** order to elucidate the regulatory mechanism,we tested the MAPK signaling pathway in *** of p38 MAPK was observed after Rg3 *** further explore the possible mechanism of Rg3 on AQP1 gene expression,we carried out the deletion analysis on the promoter region of AQP1 *** length of promoter segments were inserted into luciferase vector and the dual-luciferase assay was used to assess which fragment was crucial for Rg3's effect on *** result from this assay suggested that the - 1000 to -500 region of the promoter might be responsible for Rg3's *** study will be designed basing on these *** study uncovers the anti-migration effect of Rg3 in PC-3M cells and the role of AQP1 in the regulation process.

关键词： Ginsenoside Rg3 attenuates cell migration via inhibition of aquaporin 1 expression in PC-3M cells

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Cardioprotective effect of coptisine on the myocardial infarction induced by isoproterenol in rats

Cardioprotective effect of coptisine on the myocardial infar...

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第二届世界天然药物和传统药物药理学学术会议

作者： Lian-Hua FANG Li-Li GONG Jing GUO Yue-Hua WANG Shou-Bao WANG Hai-Lin QIN Guan-Hua DU Belting Chaoyang Hospital Affiliated with Beijing Capital Medical University Beijing 100020China Beijing Key Laboratory of Drug Targets Identification and Drug Screening Chinese Academy of Medical Science & Peking Union Medical CollegeBeijing 100050China Beijing Key Laboratory of Drug Targets Identification and Drug ScreeningChinese Academy of Medical Science & Peking Union Medical CollegeBeijing 100050China

AIM Coptisine is an isoquinoline alkaloid extracted from Coptidis *** study aims to elucidate if coptisine is responsible for cardioprotection using myocardial infarction (MI) and investigate its *** Rats were randomized into 7 groups: normal, isoproterenol (Iso), Iso + fasudil, Iso + isosorbide dinitrate and Iso + coptisine (25, 50, 100 mg· kg-1).Rats pretreated with coptisine for 21 d (ig) and injected subcutaneously with high-dose Iso (85 rag· kg-1) on the 20th, 21st day at an interval of 24 *** function and biomarkers of cardiac ischemia were assessed after *** Coptisine has strong antioxidant activity, and it can maintain cell membrane integrity, ameliorate mitochondrial respiratory dysfunction, reduce myocardial cells apoptosis, inhibit RhoA/ROCK expression in experimental MI *** The present study provides experimental evidence that coptisine has strong antioxidant activity, and it can maintain cell membrane integrity, ameliorate mitochondrial respiratory dysfunction and improve cardiac systolic/diastolic dysfunction induced by *** of RhoA/ROCK pathway was involved in coptisine induced cardioprotection effects on experimental *** should be considered as a novel adjunctive therapy for attenuating myocardial damage.

关键词： coptisine myocardial infarction isoproterenol Rho kinase

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PF11 protects neuronal function from alcohol induced neuroinflammation-oxidative stress in the adult rat following a 4-day binge ethanol exposure

PF11 protects neuronal function from alcohol induced neuroin...

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第二届世界天然药物和传统药物药理学学术会议

作者： Ya-Nan ZHAO Fang WANG Xiao-Mei HU Wei ZHANG Hui-Li WENG Yi-Fei XU Jing-Yu YANG Chun-Fu WU Department of Pharmacology Shenyang Pharmaceutical UniversityShenyang 110016China

AIM Pseudoginsenoside-F1 1 (PF11), an ocotillol-type saponin existing in American ginseng, can inhibit the ethanol-induced neuronal damage in vitro in our previous *** further investigate the effects of PF11 on alcoholism and the underlying mechanisms, we examined the effects of PF11 on neuronal function in the adult rat following a 4-day binge ethanol *** Sprague-Dawley rats were pretreated with PF11 or vehicle for 7 d, then a 4 d binge concurrent with PF11 or vehicle treatment was *** hour after last ethanol treatment, neuronal activity and uhrastructure were investigated by Fluoro-Jade B staining and electron microscopy, microglial and astrocyte response were assessed by immunohistochemistry, MDA and 3NT production of oxidative stress were detected by biochemical kits and western *** last, learning and memory abilities were explored by Morris water maze and novel object recognition test following 4 d of ethanol *** Pre-treatment with PF11 protected against ethanol-induced neurodegeneration, reduce microglial and astrocyte response and MDA and 3NT *** more, neuroprotective effects of PF11 were accompanied by prevention of deficits in spatial reversal learning ability and object recognition *** The results of the present study suggest an involvement of regulation of neuroinflammation-oxidative stress in the action of PF11 to protect against binge ethanol neurotoxicity.

关键词： pseudoginsenoside-F11 ethanol neurodegeneration neuroinflammation

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Cardiovascular protection of salvianolic acid A: epoxyeicosatrienoic acids and soluble epoxide hydrolase

Cardiovascular protection of salvianolic acid A: epoxyeicosa...

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第二届世界天然药物和传统药物药理学学术会议

作者： Shou-Bao WANG Xiao-Bin PANG Yue-Hua WANG Li ZHANG Xiu-Ying YANG Lian-Hua FANG Guan-Hua DU Beijing Key Laboratory of Drug Target Identification and Drug Screening Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

AIM To investigate whether Salvianolic acid A (SAA) exhibits its protective effects in rots through epoxide hydrolase (sEH) and Epexyeicosatrienoic acids (EETs).METHODS Heart and cerebral ischemia in rats were established through the occlusion of left anterior descending coronary artery (LAD) and middle cerebral artery occlusion (MCAO) *** 0.3, 1 and 3 mg·kg-1 was administrated before and after operation *** SAA treatment reduced infarct volume in heart and brain, improved cardiac function, and ameliorated neurological ***, the beneficial effects of SAA were attenuated by co-administration of 14,15-EEZE, a putative selective EETs ***, SAA increased the 14,15-EET levels in the blood,heart and brain of sham and ischemic *** for hydrolase activity showed that SAA 1 and 3 rag· kg-1 significantly diminished heart and brain sEH activity of ischemic rats.A fluorescent assay in vitro indicated that SAA could inhibit recombinant human sEH activity in a concentration-dependent manner (IC50 =1.62 pmol · L-1).lmmunohistochemical analysis showed that SAA 1 and 3 mg·kg-1 significantly decreased sEH protein expression in heart and hippocampus CA1 region of ischemic *** Cardiovascular protection of SAA is mediated at least in part via inhibiting sEH to increase EETs levds.

关键词： salvianolic acid A cardiovascular protection soluble epoxide hydrolase epoxyeicosatrienoic acids

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Molecular mechanism of Liu-Wei-Di-Huang decoction improving age-related cognitive deterioration

Molecular mechanism of Liu-Wei-Di-Huang decoction improving ...

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第二届世界天然药物和传统药物药理学学术会议

作者： Xiao-Rui CHENG Sheng YANG Wen-Xia ZHOU Yong-Xiang ZHANG Beijing Institute of Pharmacology and Toxicology Beijing 100850China

AIM Data from clinic trials showed Liu-Wei-Di-Huang decoction (LW) enhanced cognitive processing in normal young adults and improved age-related cognitive *** study alms to investigate the molecular mechanism of LW improving learning and memory deterioration empolying senescence accelerated mouse prone 8 (SAMP8).METHODS The ability of learning and memory of SAMP8 administrated LW were examed by step up and down test, shuttle-box test, and morris-water maze *** expression was investigated using cDNA microarray and *** techniques were used to analyses biomolecale interaction *** The chronic administration of LW to SAMP8 enhanced the memory registration and retention ability in passive avoidance performance, promoted the spatial memory ability in the water maze test and partially improved the leaning behavior in conditioned avoidance *** are 39 gene expression in hippocampus and 52 genes expression in cerebral cortex regulated by *** genes involved in the biomolecular network with 384 nodes and 383 edges, characteristic path length 4.416 and network heterogeneity *** mainly effected on pathways with nodes RPS6KA1, TICAM2, MAST2, NRXN1, NSF, STUB1 and ***, RPS6KA1, SLC17A7 and FHIT may be direct targets for LW improving age-related with cognitive *** LW enhanced cognitive processing via regulating the biomolecular network with 384 nodes and 383 edges, and its direct targets may be RPS6KA1, SLC17A7 and FHIT.

关键词： Liu-Wei-Di-Huang decoction senescence accelerated mouse learning and memory biomolecular network

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Effect of long-term alcohol consumption or high glucose and high fat diet on hypertension and kidney and liver function in rats

Effect of long-term alcohol consumption or high glucose and ...

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第二届世界天然药物和传统药物药理学学术会议

作者： Su-Hong CHEN Gui-Yuan L(U) Chao-Qun XIA Jie SU Qi ZHANG Institute of Chinese Materia Medica WenZhou Medical CollegeWenzhou 325035China Institute of Materia MedicaZhejiang Chinese Medical UniversityHangzhou 310053China

AIM Referring to the relation between hypertension and excessive drinking and high fat diet, we established the hypertension model rats by feeding with alcohol and high glucose and fat diet, which is similar to human *** SD rats, half male and half female, were divided into 3 groups according to their *** fnrst group were fed with alcohol and standard diet (the alcohol concentration increased from 5% to 22%).The second group received high glucose and high fat diet and *** third one, used as normal control group, received standard diet and *** whole experiment lasted 30 *** pressure were measured directly by tail arteries in awake *** were collected to detect the levels of serum ALT, AST, GGT, ALP, TP, ALB, UA, *** After drinking for 8 weeks, the blood pressure of SD rats started to rise *** 17 weeks, the SBP was close to 160 *** 6 weeks, the blood pressure of SD rats which fed with high glucose and high fat diet started to rise significantly and the SBP was close to 160 mmHg after 15 *** the drinking group or high glucose and high fat group, biochemical indexes which reflect liver and kidney functions appeared abnormal after 12 *** The blood pressure of SD rats could increase to some extent by long-term alcohol consumption or high glucose and high fat *** the same time, complications, such as liver and kidney injury, were also observed.

关键词： alcohol high glucose and high fat diet hypertension

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