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Endotoxin- and D-galactosamine-induced fulminant hepatic failure improved by the administration of cryptotanshinone

Endotoxin- and D-galactosamine-induced fulminant hepatic fai...

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第二届世界天然药物和传统药物药理学学术会议

作者： Li-Hua LIAN~△,Quan JIN~△,Yan-Ling WU,Ting BAI,Ji-Xing NAN~* (College of Pharmacy,Yanbian University,Yanji 133002,China)

AIM Cryptotanshinone（CTN）,one of the major constituents of tanshinones,was investigated for hepatoprotective effects in D-galactosamine（D-GalN）/lipopolysaccharide（LPS）-induced fulminant hepatic failure mouse *** CTN (20 or 40 mg·kg ) was orally administered 12 and 1 h prior to D-GalN(700 mg·kg )/LPS(10μg·kg ) *** Prelreatment with CTN markedly protected mice from lethal liver injury,which has known to be associated with a massive hepatocyte ***,CTN significantly blocked the elevation of TNF-αand alanine aminotransferase（ALT） and aspartate aminotransferase （AST） as *** also remarkably reduced DNA fragmentation in the liver,which correlated with blockade of caspase 3, caspase 8,and caspase 9 ***,increased cytosolic cytochrome c protein was reduced by *** addition,CTN significantly suppressed the activation of JNK,ERK and p38 induced by D-GalN/LPS,and phosphorylation of TAK1 as ***, CTN significantly inhibited the activation of nuclear factor（NF）-κB and suppressed production of inflammatory mediators. CONCLUSION These findings suggested that CTN prevents D-GalN/LPS-induced fulminant hepatic failure,and this protection is likely associated with its anti-apoptotic activity and the down-regulation of MAPK activity associated at least in part with suppressing NF-κB.

关键词： cryptotanshinone fulminant hepatic failure apoptosis NF-κB

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Pinocembrin protects neurons against beta-amyloid-induced toxicity through inhibiting RAGE-mediated pathways and regulating mitochondrion-mediated apoptosis

Pinocembrin protects neurons against beta-amyloid-induced to...

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第二届世界天然药物和传统药物药理学学术会议

作者： Rui LIU,Cai-Xia WU,Dan ZHOU,Li ZHANG,Tian-Tai ZHANG,Guan-Hua DU~* (Beijing Key Laboratory of Drug Target and Screening Research,Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100050,China)

AIM To investigated the effects and potential mechanisms of pinocembrin on the cognitive function against Aβ-induced *** Mice received an intracerebroventricular fusion of Aβ.The behavioral performance,cerebral cortex neuropil ultrastructure,and neuronal degeneration were *** mechanism was conducted using fluorescence-based multiparametric technologies on a high-content analysis *** Pinocembrin improved cognitive function,preserved ultrastructural neuropils and decreased neurodegeneration of cerebral cortex in Aβ-treated *** did not provide sufficient effect on inhibiting Aβ production and scavenging intracellular ROS,but significantly inhibited the upregulation of RAGE transcripts and protein ***,pinocembrin depressed the activation of p38MAPK-MK2-HSP27 and SAPK/JNK-c-Jun pathways and the downstream NF-κB inflammatory *** also alleviated mitochondrial dysfunction and regulated mitochondrion-mediated apoptosis. CONCLUSION Pinocembrin showed cognitive improvement in AD *** mechanisms were illustrated on RAGE-dependent transduction inhibition and mitochondrion protection.

关键词： Alzheimer's disease amyloid-βpeptide RAGE pinocembrin

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Effect of Anacyclus pyrethrum on sexual and reproductive dysfunction in hyperglycemic male rats

Effect of Anacyclus pyrethrum on sexual and reproductive dys...

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第二届世界天然药物和传统药物药理学学术会议

作者： Vikas SHARMA V.K.DIXIT Natural Product Research Laboratory Department of Pharmaceutical SciencesDr.H.S.Gour UniversitySagar 470003 MPIndia

AIM Anacydus pyrethrum is considered as a sexual tonic in ayurvedic system of medicine with potent antioxidant and adaptogenic *** ethanolic extract of the herb was evaluated for its effectiveness against streptozotocin induced hyperglycemic stress and subsequent sexual dysfunction due to hyperglycemia in male *** Five groups with eight male rats in each group were used for the present study and the study was carried out for 28 *** body and organ weights of the animals were recorded. Behavioral analysis of rats was undertaken to observe the effect on mount,ejaculation and intromission（latencies and frequencies）. Blood glucose and serum testosterone levels were determined after 28 d of treatment with Anacydus pyrethrum at 50,100 and 150 mg·kg *** deleterious effect of sustained hyperglycemia and associated stress was significantly ameliorated in animals treated with ethanolic extract of Anacydus *** The Anacydus pyrethrum treatment was helpful in ameliorating the damage of sustained hyperglycemia evidenced in the principle parameters *** sexual behavior,sperm count,penile erection index and seminal fructose *** and anabolic activities of the extract under investigation could be a major attribute in preserving the sexual functions in hyperglycemic male *** The study validates the use of Anacydus pyrethrum in traditional medicine for curing diabetes induced sexual dysfunction and compromised sexual potency.

关键词： Anacyclus pyrethrum sexual dysfunction sperm count diabetes vajikarana

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Inhibition of the extract of Radix Scutellariae and Trollius chinensis on influenza viruses in vitro

Inhibition of the extract of Radix Scutellariae and Trollius...

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第二届世界天然药物和传统药物药理学学术会议

作者： Ping HE~1,Xiao-Lan CUI~2,Shan-Shan GUO~2,Lian-Da LI~1,Wei-Feng YANG~1 (1.Research Center of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China 2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

AIM To observe the inhibition of the extract of Radix Scutellariae and Trollius chinensis on four kinds of influenza viruses in *** Cytopathic effect（CPE） observation was used to observe the inhibitory effect of the extract of Radix Scutellariae and Trollius chinensis on influenza a（HlNl） virus FM1 strain,influenza a（HlNl） virus PR8 strain,influenza a （H3N2） virus brisbane10 strain and influenza B virus Xiushui *** The extract of Radix Scutellariae and Trollius chinensis had inhibiting effects on cytopathic effect of MDCK cells caused by the infection of H1N1 virus PR8 strain,influenza a （H3N2） virus brisbane10 strain and influenza B virus Xiushui strain,and their 50%inhibiting concentration(IC ) were 0.67,0.47 and 0.67 g·*** The extract of Radix Scutellariae and Trollius chinensis has antiviral effect on influenza viruses in vitro.

关键词： Radix Scutellariae Trollius chinensis influenza virus

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Preparation and characterization of a new certified reference material of puerarin(GBW09511)

Preparation and characterization of a new certified referenc...

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第二届世界天然药物和传统药物药理学学术会议

作者： Yang LU~(1,2),Ning-Bo GONG~1,Yong-Hui GUO~1,Ning YANG~1,Zhao-lin GAO~(1,2),Guan-Hua DU~(1,2) (1.Center of Pharmaceutical Polymorph,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China 2.Key Laboratory of Polymorph Pharmaceutical of Shandong Province,Tengzhou 277500,China)

AIM To prepare and research the characteristics of puerarin as a certified reference materials(CRMs) for the crucial quality assurance to validate measurementmethods which deliver reliable and accurate *** Sample preparation, homogeneity study,stability study,value characterization,and evaluation of uncertainty of a new CRM of puerarin are *** homogeneity was checked by differential scanning calorimetry *** under differentstorageconditions. RESULTS The CRM described above was homogeneousandstable at 25℃for at least one *** certified value of puerarin CRM is 99.66%with an extended uncertainty of 0.48%(k = 2).CONCLUSION The new CRM of puerarin can be used to validate analytical methods,improve the accuracy and comparability of measurement data,establish meteorological traceability of analytical resultsas well as control the quality of puerarin in relevant food and pharmaceutical formulations.

关键词： puerarin differential scanning calorimetry

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Synergistic proliferation-inhibiting effects of GP and paclitaxel on MGC80-3 cells

Synergistic proliferation-inhibiting effects of GP and pacli...

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第二届世界天然药物和传统药物药理学学术会议

作者： Cong WANG,Sai-Qi WANG,Ran DONG,Liu-Bin GUO,Hong-Min LIU~* (School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001,China)

AIM To investigate the proliferation-inhibiting effects and mechanisms of the combination of an active ingredient from Rabdosia rubescens GP and paclitaxel（PTX） on human gastric cancer MGC80-3 cells in *** After administration of GP(0.5 -4μmol·L ) or PTX(1-8 nmol·L ),or the combination for 48 h,MTT assay was used to detect the inhibition rates and the drug combination was determined by Chou-Talalay method through calculating combination index（CI） *** expression of p53 was evaluated by Western *** The inhibition rates of combination groups were remarkablely higer than that of the respective single-agent *** the inhibition rate was higer than 30%,the combination of GP and PTX produced the CI below 1,suggesting a synergy between GP and PTX in inhibiting cell *** blot results showed that the combination treatment caused an increased expression of p53 in a dose-dependent manner compairing to either GP or PTX *** GP synergistically enhanced the proliferation-inhibiting effect of PTX in human gastric cancer MGC80-3 cells in vitro and the potential mechanism may be the activation of p53 pathway.

关键词： GP MGC80-3 combination index p53

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Effects of HSW on mice model of Parkinson's disease induced by MPTP

Effects of HSW on mice model of Parkinson's disease induced ...

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第二届世界天然药物和传统药物药理学学术会议

作者： Ru-Yi ZHANG,Li ZHANG,Yan-Chuan WU,Yu-Lan WANG,Lin LI~* (Department of Pharmacology,Xuanwu Hospital of Capital Medical University/Beijing Geriatric Medical Research Center Key Laboratory for Neurodegenerative Diseases of Ministry of Education,Beijing 100053,China)

AIM To observe the effects of HSW on behavior and content of dopamine（DA）,DOPAC and HVA in stratium of mice treated with l-methyl-4-phenyl-l,2,3,6-tetrahydropyridme（MPTP）.METHODS C57BL mice were treated with MPTP. The pole test and Rota-Rod test were conducted to determine the motor harmony;spontaneous motor activity was determined to test the excitability of *** contents of DA,DOPAC and HVA in stratium of mice were determined with high performance liquid chromatograph （HPLC）.RESULTS Model mice intraperitoneally injected with MPTP at the dose of 30 mg·kg for 5 d showed disability in pole test and Rota-Rod test and decreased spontaneous motor *** increased the spontaneous motor activity,shortened the climbing time of pole test,and increased the content of dopamine in *** Mice treated with MPTP manifested Parkinsonism *** can ameliorate abnormal behaviors of model mice and may have a good perspective in treatment of PD.

关键词： HSW dopamine

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Effect of resveratrol on neonatal rat cardiac myocytes exposure to high-glucose

Effect of resveratrol on neonatal rat cardiac myocytes expos...

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第二届世界天然药物和传统药物药理学学术会议

作者： Ji-Liang WU,Chao LIU,Shuang GUO (Hubei Province Key Laboratory on Cardiovascular,Cerebrovascular,and Metabolic Disorders,Xianning University, Xianning 437100,China)

AIM Through culturing cardiac myocytes of neonatal Sprague-Dawley rats in vitro,observe the effects of resveratrol （Res） on cardiac myocytes exposure to high-glucose（HG）,and then explore the possible *** Ventricular myocytes were isolated from neonatal rat and *** cells were divided into three groups:normal-glucose(NG,5.5 mmol·L ) group,the HG 30 mmol·L group,and the HG + Res(50μmol·L ) *** cell viablity was calculated by CCK8 measurement. The activity of LDH and the fluorescence intensity of ROS were determined by biochemical *** and FCM detected the rate of apoptosis;Bcl-2 and Bax protein expression were measured by Western *** Compared with NG group,the LDH level and ROS generation increased significantly in HG *** could inhibit the releasing of LDH and attenuate intracellular ROS generation, ***,HG induced apoptotic cell *** decreased the apoptosis evidently through up-regulating Bcl-2 and down-regulating Bax proteins *** It seemed that Res could reduce cardiac myocytes apoptosis exposure to *** underlying mechanism may be result from its capacity of scavenging ROS and up-regulating the ratio of Bcl-2 and Bax ratio.

关键词： resveratrol high-glucose damage reactive oxygen species apoptosis

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Preparation and characterization of a new certified reference material of puerarin (GBW09511)

Preparation and characterization of a new certified referenc...

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第二届世界天然药物和传统药物药理学学术会议

作者： Yang LU Ning-Bo GONG Yong-Hui GUO Ning YANG Zhao-lin GAO Guan-Hua DU Center of Pharmaceutical Polymorph Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Key Laboratory of Polymorph Pharmaceutical of Shandong Province Tengzhou 277500China Center of Pharmaceutical PolymorphInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

AIM To prepare and research the characteristics of puerarin as a certified reference materials(CRMs) for the crucial quality assurance to validate measurementmethods which deliver reliable and accurate *** Sample preparation,homogeneity study, stability study, value characterization, and evaluation of uncertainty of a new CRM of puerarin are *** homogeneity was checked by differential scanning calorimetry *** under *** The CRM described above was homogeneousandstable at 25℃ for at least one *** certified value of puerarin CRM is 99.66% with an extended uncertainty of 0.48% (k =2).CONCLUSION The new CRM of pucrarin can be used to validate analytical methods, improve the accuracy and comparability of measurement data, establish meteorological traceability of analytical resuhsas well as control the quality of puerarin in relevant food and pharmaceutical formulations.

关键词： puerarin differential scanning calorimetry

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Endothelium-dependent and-independent relaxation of rat aorta induced by extract from Schizophyllum commune

Endothelium-dependent and-independent relaxation of rat aort...

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第二届世界天然药物和传统药物药理学学术会议

作者： Su-Juan LI Xiao-Xia PAN Cui YANG Key Laboratory of Chemistry in Ethnic Medicinal Resources State Ethnic Affairs Commission & Ministry of EducationYunnan University of NationalitiesKunming 650500China

AIM The relaxing effect of the extract from Schizophyllum commune (SC) on rat aorta as well as the underlying mechanisms were *** Dried SC was extracted with 50%, 90% ethanol or deionized *** the administration of SC, the isometric tension was measured in rat aortic rings with or without *** In endothelium-intact or in endothelium-denuded rat aortic rings, the water extract of SC induced significant relaxations, which were markedly decreased by the pretreatments of L-NAME, indomethacin, RP-cAMP, methylene blue and high concentration of *** Ca2 +-free solution, incubation of the aortic tings with SC significantly inhibited the Ca2 +-dependent *** The water extract of SC induces an endothelium-dependent and-independent relaxation in rat *** activations of NO-cGMP, PGI2-cAMP pathways and K +channels, and the inhibitions of Ca2 + channels may be involved in the vasorelaxing effect of SC.

关键词： Schizophyllum commune vasorelaxation rat aorta

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