Paper-based enzyme immobilization for flow injection electrochemical biosensor integrated with reagent-loaded cartridge towards portable device was developed.A paper disk was immobilized with enzyme,then it was integr...
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Paper-based enzyme immobilization for flow injection electrochemical biosensor integrated with reagent-loaded cartridge towards portable device was developed.A paper disk was immobilized with enzyme,then it was integrated in a flow cell as an electrochemical *** tube reagent-loaded cartridge was integrated into the system,complicated procedure was simplified as one-click operation towards development for point-of-care *** this research,glucose oxidase (GOx) was employed as a model enzyme,silver ion as an inhibition reagent for GOx,EDTA as a regeneration *** GOx was inhibited by silver ions,glucose was introduced for electrochemical measurements before and after inhibited enzyme regeneration,the difference was caused by silver *** modular device has great potential for other applications,*** of enzyme activity and *** platform based on double-test mode provided accurate results due to elimination of an average or control value in comparison with routine approaches.
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