A taxonomic study was carried out on strain D104,which was isolated from deep-sea subsurface sediment of the Arctic *** bacterium was Gram-negative,oxidase negative and catalase weak positive,rod shape,motile by polar...
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A taxonomic study was carried out on strain D104,which was isolated from deep-sea subsurface sediment of the Arctic *** bacterium was Gram-negative,oxidase negative and catalase weak positive,rod shape,motile by polar *** was observed at salinities from 0.5 to 6%and temperatures from 4 to 37°C,and it was capable of aesculin hydrolysis,but unable to reduce nitrate to *** analysis based on 16S rRNA gene sequences indicated that strain D104 belonged to the genus Marinomonas,with highest sequence similarity of 97.72%to Marinomonas ushuaiensis DSM 15871,followed by other 20 species of genus Marinomonas(94.51- 96.94%),while the other genera were all below 91.00%.The ANIm value and DNA-DNA hybridization estimate value between strain D104T and *** DSM 15871were 84.24%and 20.80 + 2.33%*** principal fatty acids were Sum In Feature 3(C)(27.11%),Sum In Feature 8(C_(18:1ω7c/Cω6c)(39.67 %),C(12.32%) and C3OH(5.77%).The G+C content of the chromosomal DNA was 44.8 mol%. The respiratory quinone was determined to be Q8(100%).Phosphatidylglycerol(PG), Phosphatidylethanolamine(PE),aminolipid(AL) and Phospholipid(PL) were *** combined genotypic and phenotypic data show that strain D104 represents a novel species of genus Marinomonas, for which the name Marinomonas projundimaris *** proposed,with the type strain D104 (=CGMCCNO.1.12597 = LMG27696=MCCC 1A07573).
A Gram-stain-negative,yellow-pigmented,aerobic bacterial strain,designated LY02,was isolated from coastal seawater in Xiamen,Fujian Province of *** was observed at temperatures from 4 to 44℃,at salinities from 0 to 9...
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A Gram-stain-negative,yellow-pigmented,aerobic bacterial strain,designated LY02,was isolated from coastal seawater in Xiamen,Fujian Province of *** was observed at temperatures from 4 to 44℃,at salinities from 0 to 9%and at pH from 5 to *** analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus Altererythrobacter in the family *** strain LY02 was most closely related to Altererythrobacter marensis MSW-14 (97.2%sequence similarity),followed by Altererythrobacter ishigakiensis JPCCMB0017(97.1%), Altererythrobacter epoxidivorans JCS350(97.1%) and Altererythrobacter luteolus SW-109(97.0%). The dominant fatty acids were Cω7c(61.65%),Cω6c(10.07%) and Summed Feature 3(comprising Cω7c and/or Cω6c,9.63%),which accounted for 81.35%.DNA-DNA hybridization showed that strain LY02 with *** MSW-14,*** JPCCMB0017,*** JCS350 and *** SW-109 possessed a low DNA-DNA relatedness(27.7±0.8%,30.2±1.4%,21.3±1.5%and 18.7±1.2%,respectively).The G+C content of the chromosomal DNA was 61.2 mol%.The predominant respiratory quinone was ubiquinone-10(Q-10).According to its morphology,physiology,fatty acid composition and 16S rRNA sequence data,the novel strain most appropriately belongs to the genus Altererythrobacter,but can readily be distinguished from known Altererythrobacter *** name Altererythrobacter xiamenense *** proposed(type strain LY02= CGMCC 1.12494= KCTC 32398=NBRC109638).
A taxonomic study was carried out on strain HYC-10,which was isolated from the intestinal tract contents of a flathead mullet,Mugil cephalus,captured from the sea off Xiamen Island,*** bacterium was observed to be Gra...
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A taxonomic study was carried out on strain HYC-10,which was isolated from the intestinal tract contents of a flathead mullet,Mugil cephalus,captured from the sea off Xiamen Island,*** bacterium was observed to be Gram positive,oxidase and catalase positive,rod shaped,and motile by subpolar *** bacterium was found to grow at salinities of 0-12%and at temperatures of 8-45℃. The isolate was found to hydrolyze aesculin and gelatin,but was unable to reduce nitrate to nitrite. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HYC-10 belongs to the genus Bacillus,with highest sequence similarity(99.3%) to Bacillus aerophilus 28K,Bacillus stratosphericus 41KF2a,and Bacillus altitudinis DSM 21631,followed by Bacillus safensis DSM 19292 (99.5%) and Bacillus pumilus DSM 27(99.5%),while the sequence similarities to others were all below 97.6%.The genomic ANIm values between strain HYC-10 and three type strains {*** DSM 21631,*** DSM 19292 and *** DSM 27) were determined to range from 89.11%to 91.53%.The DNA-DNA hybridization estimate values between strain HYC-10 and the three type strains were from 36.60%to 44.00%.The principal fatty acids identified were iso-Cis:o(39.1%),anteiso-C]5:o (22.7%),iso-C(13.1%),C(6.1%),anteiso-C(5.8%) and iso-C(5.1%).The G+C content of the chromosomal DNA was determined from the draft genome sequence to be 41.3mol%.The respiratory quinone was determined to be MK-7(100%).Phosphatidylglycerol,diphosphatidylglycerol, aminoglycolipid,two glycolipids and two unknown phospholipids were found to be *** combined genotypic and phenotypic data show that strain HYC-10 represents a novel species of the genus Bacillus, for which the name Bacillus xiamenensis *** proposed,with the type strain HYC-10~1(=CGMCC NO.1.12326~1 = LMG 27143=MCCC 1A00008).
A taxonomic study was carried out on strain P73,which was isolated from deep-sea sediment of the Indian Ocean by enrichment of *** can degrade biphenyl,naphthalene,2-methylnaphthalene, 2,6-dimethylnaphthalene,acenapht...
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A taxonomic study was carried out on strain P73,which was isolated from deep-sea sediment of the Indian Ocean by enrichment of *** can degrade biphenyl,naphthalene,2-methylnaphthalene, 2,6-dimethylnaphthalene,acenaphthene,anthracene,phenanthrene,dibenzothiophene,dibenzofuran, fluorene,4-methyldibenzothiophene and fluoranthene,but not pyrene or *** intermediate metabolites of fluoranthene degradation were examined with ***-1(2H)-one, acenaphthenequinone,1,2-dihydroxyacenaphthylene and 1,8-naphthalic anhydride were detected as the main intermediates,indicating that the degradation of fluoranthene was initiated by dioxygenation at the C-7,8 *** analysis based on 16S rRNA gene sequences showed that strain P73 formed a clade with the genus Celeribacter and Huaishuia within the family Rhodobacteraceae,with highest sequence similarity of 96.98%to Celeribacter neptunius H14,followed by Huaishuia halophile ZXM137 (96.42%).The bacterium was Gram-negative,oxidase and catalase positive,rod shaped and non-motile. Growth was observed at salinities from 0.5 to 12%and at temperatures from 10 to 41°*** principal fatty acids of strain P73 were Sum In Feature 8(Cω7c/ω6c)(58.6%),Cω8c cyclo(17.4%) and C (7.9%).The respiratory quinone was Q-10(100%).The lipids were phosphatidylglycerol(PG),aminolipid (AL),two phospholipids(PL),glycolipid(GL) and one unknown lipid(L).The G+C content of the chromosomal DNA was 66.0 mol%.The combined genotypic and phenotypic data show that strain P73T represents a novel species of the genus Celeribacter,for which the name Celeribacter indica *** proposed,with the type strain P73(=LMG 27600 = DSM 27257= MCCC 1A01112).Phylogenetic study and existing phenotypic information also show that Huaishuia halophila should be transferred to the genus Celeribacter as Celeribacter halophila ***.(type strain ZXM137 =CGMCC 1.8891~ T = LMG 24854).To our knowledge,this is the first report that bacterium belonging
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