Objectives: In this study, we describe a novel method for treating patients with complete spinal cord injuries (SCI) by utilizing purified CD34+ and CD133+ stem cells (SCs), and demonstrate the safety and results of a...
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Objectives: In this study, we describe a novel method for treating patients with complete spinal cord injuries (SCI) by utilizing purified CD34+ and CD133+ stem cells (SCs), and demonstrate the safety and results of autologous mesenchymal stem cell transplantation (SCT) in treated patients during the follow up period Materials and Methods: In this study, we included 40 patients (M, F) who had complete SCI (ASIA-A). In twenty patients we utilized
Background: Spinal Cord Injury (SCI) is one of the most devastating injuries that afflict young people at the height of their social and working life. It is a multi-system injury which leads to a significant morbidity...
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Background: Spinal Cord Injury (SCI) is one of the most devastating injuries that afflict young people at the height of their social and working life. It is a multi-system injury which leads to a significant morbidity and mortality. SCI repair is one of the biggest challenges in modern medicine and this complex human experiment in neural repair for SCI individuals must be proven safe and effective by the highest standard evident-based medicine.
OBJECTIVE: Spasticity is an abnormal increased muscle tone as results of neurological disorders such as stroke and spinal cord injury. Clinically, spasticity is being evaluated through several semi-qualitative scales ...
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OBJECTIVE: Spasticity is an abnormal increased muscle tone as results of neurological disorders such as stroke and spinal cord injury. Clinically, spasticity is being evaluated through several semi-qualitative scales such as Ashworth score, Modified Ashworth Score, Tardio and Barthly. Although semi-qualitative scales have been used to evaluate the magnitude of spasticity, these scales are not sensitive and quantitative. Recently, in
OBJECTIVE: Gait training is one of the goals of rehabilitation after incomplete SCI. As a result, new rehabilitation approaches (new device techniques) have focused on training and/or regaining walking abilities. Duri...
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OBJECTIVE: Gait training is one of the goals of rehabilitation after incomplete SCI. As a result, new rehabilitation approaches (new device techniques) have focused on training and/or regaining walking abilities. During the last decade, robotic gait devices have been used as a new device for walking training in neurological rehabilitation. Although the effectiveness of robotic gait devices have been demonstrated in several reports, one of the main
The idea that decoding the structure of genetic defect of pathological neural cells (NCs) in major neural disorders (NDs) will lead to their efficient treatment has long prevailed in classical neurology. Successfully ...
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The idea that decoding the structure of genetic defect of pathological neural cells (NCs) in major neural disorders (NDs) will lead to their efficient treatment has long prevailed in classical neurology. Successfully accomplished The Human Genome Project demonstrated that decoding genome of a somatic cell (SomC) did not approximate understanding of pathogenesis and treatment of incurable human diseases (cancer, diabetes, SCI). Consequently, the
Here we summarize 16 years of our clinical experience of using various cell systems and technologies for the therapy of spinal cord injury (SCI) in humans and to perform comparative analysis of long-term outcomes. In ...
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Here we summarize 16 years of our clinical experience of using various cell systems and technologies for the therapy of spinal cord injury (SCI) in humans and to perform comparative analysis of long-term outcomes. In 1996 we first published the results of the therapy with fetal neural cells (FNCs) in combat mine-blast injury of spinal cord and brain (Bryukhovetskiy et al, 1996). For FNCs transplantation we first applied the method of intrathecal
In the last years, the application of "nanotechnology "to the field of "medicine" surely represented the most innovative strategy to cop0e with diseases and it could be named as nanomedicine applie...
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In the last years, the application of "nanotechnology "to the field of "medicine" surely represented the most innovative strategy to cop0e with diseases and it could be named as nanomedicine applied to difficult-to-treat diseases. As known, in this field of research, the most important goal to be reached is an increase in selectivity and specificity of drug action. Several results with stimulating findings in preclinical or clinical phases have
Gene regulation remains one of the major challenges for the clinical application of gene therapy. In the present study, we developed an ex vivo gene delivery system using tetracycline (Tet)-regulated lentiviral vector...
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Gene regulation remains one of the major challenges for the clinical application of gene therapy. In the present study, we developed an ex vivo gene delivery system using tetracycline (Tet)-regulated lentiviral vectors and human bone marrow-derived mesenchymal stem cells (hMSCs). We first generated a Tet-On bicistronic lentiviral vector system based on the tetracycline dependent transactivator rtTA2S-M2. This system allows for simultaneous expression
Pathophysiology of the Central nervous system (CNS) injury is complex and involves damage to neuronal, glial and endothelial cells leading to brain dysfunction [1-3]. Studies carried out in our laboratory since 1977 s...
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Pathophysiology of the Central nervous system (CNS) injury is complex and involves damage to neuronal, glial and endothelial cells leading to brain dysfunction [1-3]. Studies carried out in our laboratory since 1977 shows that disruption of the blood-brain barrier (BBB) to proteins play crucial roles in precipitating brain edema formation and cell damage [3-6]. Thus, restoration of the BBB with drugs, antibodies, stem cells or other therapeutic agents
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