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检索条件"任意字段=第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会"

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Effect of erigeron breviscapus (Vant.) Hand-Mazz pretreatment on pathology and oxyradical level following spinal cord ischemia-reperfusion injury in rabbits

Effect of erigeron breviscapus (Vant.) Hand-Mazz pretreatmen...

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Feng-Tao Li Dong Wang Hao-peng Li Xi-Jing He Bin Cheng

Objective To investigate the effect of erigeron breviscapus (Vant.) Hand-Mazz(erigeron breviscapus) pretreatment on pathology and oxyradical level in spinal cord after ischemia-reperfusion(I/R) injury in rabbits. Methods A total of 40 New Zealand white rabbits were randomly divided into three groups: sham-operation group (10 rabbits treated with procedure of just exposing abdominal aorta without occlusion), control group (15 rabbits underwent ischemia for 50 minutes and treated with matched saline) and experiment group (15 rabbits underwent ischemia for 50 minutes and treated with erigeron breviscapus (9mg/kg) injection before ischemia). Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in the spinal cord were examined at 6 and 24 hours after I/R, respectively. The morphological change and the number of the spinal cord anterior horn motor neurons were observed and counted under light microscope and electron microscope respectively. Results The level of MDA was markedly decreased and SOD activity was increased in the experiment group compared with the control group (P<0.01). Compared with control group, the number of the motor neurons in the experiment group significantly increased at 24h after I/R (P<0.01) and the morphous of the motor neurons improved. Conclusion: Erigeron breviscapus can reduce oxyradical product and the apoptosis of nerve cells, and protect nerve tissue structure and function after spinal cord I/R.

关键词： ischemia-reperfusion injury malondialdehyde superoxide dismutase erigeron breviscapus （Vant.） Hand-Mazz

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The effects of electrospun TSF nanofiber diameter and alignment on growth, migration and neural differentiation of mesenchymal stem cells

The effects of electrospun TSF nanofiber diameter and alignm...

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Dandan Zhou Jing Qu Xiaojing Xu Feng Zhang Lihong He Rong Ye Ziyu Zhu Baoqi Zuo Huanxiang Zhang Medical College of Soochow University Department of Cell BiologyJiangsu Key Laboratory of Stem Cell Research College of Textiles and Clothing Engineering Soochow UniversityNational Engineering Laboratory for Modern Silk

Silk fibroin is a potential candidate biomaterial for nerve tissue engineering applications. However, the effects of fiber diameter and alignment of silk fibroin scaffold on growth and neural differentiation of mesenchymal stem cells (MSCs) remain largely unclear. In this study, we prepared the Tussah silk fibroin (TSF) nanofibers with different diameter and alignment by electrospinning and analyzed the growth, migration, and neural differentiation of MSCs on these TSF scaffolds. Our results indicated that aligned and smaller diameter (400 nm) TSF scaffolds are more favorable to the growth and migration of MSCs. Moreover, 400 nm aligned TSF scaffolds significantly promote the neuronal differentiation and neurite outgrowth projection of MSCs-derived neurons as compared with 800 nm or random TSF scaffolds, suggesting that the topography of TSF scaffolds, including fiber diameter and alignment, strongly affect the morphology and development of MSCs-derived neurons. Importantly, MSCs-derived neurons usually grow along the long axes of the aligned TSF scaffolds and neurite outgrowth is parallel to the aligned fibers. In conclusion, this study indicates that aligned 400 nm TSF scaffolds plays a significant role in supporting the growth, migration and differentiation of MSCs and can be promising scaffold used for future nerve tissue engineering.

关键词： tussah silk fibroin fiber diameter fiber alignment mesenchymal stem cell migration neural differentiation

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Control of olfactory ensheathing cell behaviors by electrospun silk fibroin nanofibers

Control of olfactory ensheathing cell behaviors by electrosp...

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Zhihai Fan Feng Zhang Peng Wu Zonggang Xie Liubing Li Peng Zhang Baoqi Zuo Qiang Lu Yixin Shen Qirong Dong Huanxiang Zhang Department of Orthopedics the Second Affiliated Hospital of Soochow University Medical College of Soochow University Department of Cell BiologyJiangsu Province Key Laboratory of Stem Cell Research College of Textile and Clothing Engineering National Engineering Laboratory for Modern SilkJiang SuPeople's Republic of China

Olfactory ensheathing cells (OECs), the only glial cell type that normally penetrates the transition zone between the peripheral and central nervous systems, are one of the most promising candidates for cell transplantation in repairing spinal cord injury (SCI). However, we need to be able to manipulate and regulate the OECs behavior to make these cells effective in cell transplantation. In the present study we assessed the response of rat OECs to different variants of nanofibrous silk fibroin mats with regard to cell morphology, adhesion, proliferation and migration, and the related gene and protein expression. Results showed that OECs are easier to adhere and spread on TSF nanofibers than on SF nanofibers, resulting in a higher rate of cell proliferation, gene and protein expression examined by RT-PCR and ELISA. In addition, the morphology of OECs on micro fibers is mainly polygonal with short protrusions, whereas the OECs on nano fibers exhibit bipolar shape with long protrusions that align along the fibers, especially when aligned nanofibers are employed. Moreover, OECs on SF nanofibers migrate more efficiently than those on *** on the experimental results, the morphology, adhesion, spread, gene and protein expression, and migration of OECs could be modulated and regulated by adjusting the contents and structure of SF nanofiber scaffolds, shedding light on the control of OECs behavior in nerve tissue engineering and thus the future therapeutic intervention for nerve repair after injury.

关键词： Olfactory ensheathing cells Electrospun fibers Tussah silk fibroin Bombyx mori silk fibroin Fiber diameter Alignment

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Neuronal spike sorting based on radial basis function neural networks

Neuronal spike sorting based on radial basis function neural...

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Alireza Khoshnevisan MD. Mostafa Taghavi Kani MSc. Department of Neurosurgery Shariati Hospital Department of Biomedical Engineering & Medical PhysicsTehran University of Medical SciencesTehranIran

Background: Studying the behavior of a society of neurons, extracting the communication mechanisms of brain with other tissues, finding treatment for some nervous system diseases and designing neuroprosthetic devices, require an algorithm to sort neuralspikes automatically. However, sorting neural spikes is a challenging task because of the low signal to noise ratio (SNR) of the spikes. The main purpose of this study was to design an automatic algorithm for classifying neuronal spikes that are emitted from a specific region of the nervous system. Methods: The spike sorting process usually consists of three stages: detection, feature extraction and sorting. We initially used signal statistics to detect neural spikes. Then, we chose a limited number of typical spikes as features and finally used them to train a radial basis function (RBF) neural network to sort the spikes. In most spike sorting devices, these signals are not linearly discriminative. In order to solve this problem, the aforesaid RBF neural network was used. Results: After the learning process, our proposed algorithm classified any arbitrary spike. The obtained results showed that even though the proposed Radial Basis Spike Sorter (RBSS) reached to the same error as the previous methods, however, the computational costs were much lower compared to other algorithms. Moreover, the competitive points of the proposed algorithm were its good speed and low computational complexity. Conclusion: Regarding the results of this study, the proposed algorithm seems to serve the purpose of procedures that require real-time processing and spike sorting.

关键词： Neuronal spike sorting based on radial basis function neural networks

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Research of PEG-TAT-modified cyclosporine A-loaded PLGA/polymeric liposomesto Cross the Blood-Spinal Cord Barrier for treating Spinal Cord Injury

Research of PEG-TAT-modified cyclosporine A-loaded PLGA/poly...

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Yu-Lin Li Shi-Qing Feng Department of Orthopaedics Tianjin Medical University General Hospital

Cyclosporine-A (CsA) is an immunosuppressant agent which has been proven as a neuroprotective drug. But its inability to readily cross the blood-spinal cord barrier (BSCB) constrains the application of CsA in clinic to treat spinal cord injury (SCI). Our group recently tested the ability of novel PEG-TAT-modified CsA-loaded cationic multifunctional polymeric liposome-PLGA core/shell nanoparticles (PLGA/CsA PLs) to carry and deliver CsA across the BSCB to treat SCI. The PLGA/CsA PLs were successfully constructed with particle size of 251.4±2.2nm, zeta potential of 25.86±3.33 *** loading capacity of the uncoated PLGA/CsA corewas about 11.5%. In vitro drug release study confirmed sustained release of CsA from PLGA/CsA PLs compared to the uncoated PLGA/CsA core. While in vivo study showed that in injured animals receiving PLGA/CsA PLs through the tail vein, significant up regulation of GAP-43 expression and more GAP-43 stained neurons were found compared to those receiving CsA or vehicle alone. This increase in GAP-43-positive cells was paralleled by a significant improvement in neurological function evaluated by Basso-Beattie-Bresnahan (BBB) open-field test. And PLGA/CsA PLs to which FITC was attached were successfully aggregated in the intact spinal cord4h after injection. Our data suggests thatPLGA/CsA PLs could be regarded as a new method for the treatment of SCI.

关键词： Cyclosporine-A (CsA) polymericliposomes (PLs) polyethylene glycol (PEG) transactivating-transduction protein (TAT) spinal cord injury (SCI) blood-spinal cord barrier (BSCB) growth-associated protein (GAP-43)

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Local Subcutaneous Cobalamin Injection for Subacute Herpetic Neuralgia:Preliminary results of an observer-blind randomized controlled trial study

Local Subcutaneous Cobalamin Injection for Subacute Herpetic...

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Gang Xu Yan Feng Wei-Zhen Tang Zhen-Xian Yao Xiang-Ting Guo Zhen Sun Zhi-An Wang Rui Chu Zhong-Wei Lv Department of Rehabilitation Medicine Affiliated Tenth People's HospitalTongji University School of Medicine

Objectives: To explore if there are any beneficial effects from local subcutaneous Methylcobalamin injection in reducing pain intensity and interference with quality of life (QoL) among patients with subacute herpetic neuralgia (SHN). Methods: 89 subjects ≥50 years of age with SHN and worst pain ≥5 on the pain intensity in the past 24 h were randomized to receive either local subcutaneous MeB 12 injection (N=44) or oral MeB 12 administration (N=45) for a 4-week study. The efficacy variables were evaluated the changes in pain intensity and interference with ADL at baseline and on the 7 th , 14 th and 28 th during treatment. Results: After 4-weeks treatment, 18 (40.91%) of the 44 subjects who received local injection had a "severe-to-mild" and 6 (13.64%) "severe-to-moderate" pain grades change compared with 3 (6.67%) of the 45 "severe-to-mild" and 18 (40.00%) "severe-to-moderate" change who treated with oral administration. 35 (79.55%) subjects had mild pain in local injection group compared with 7 (15.56%) subjects in oral administration group at the endpoint. Local MeB 12 injection is more effective than oral MeB 12 administration to control pain with SHN. Conclusion: In SHN, methylcobalamin, a neurotrophic agent had more significance analgesic effect on zoster-related pain. Local subcutaneous injection is more effective than systemic medication. The MeB 12 subcutaneous injection is a promising local treatment for SHN, which has efficacy for relieving pain and appears to be tolerable, safe. This clinical trial provides a foundation for evidence-based treatment for pain in SHN.

关键词： Subacute herpetic neuralgia Cobalamin Local subcutaneous injection Randomized clinical trial

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Establishing a Clinical Grade Cell Manufacturing Facility in an Academic Center

Establishing a Clinical Grade Cell Manufacturing Facility in...

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Hamid Reza Aghayan Babak Arjmand Hooshang Saberi Abbas Norouzi-Javidan Seyed Hassan Emami-Razavi Brain and Spinal Cord Injury Research Center Tehran University of Medical sciences Endocrinology and Metabolism Research Center Tehran University of Medical sciences

Cell-based therapies have grown dramatically in power and scope in recent years. Cell manufacturing for clinical applications is a unique form of biologics manufacturing that relies on maintenance of stringent work practices designed to ensure product consistency and prevent contamination. More extensive, prolonged laboratory processes involve greater risk of complications and possibly adverse events for the recipient, and so the need for control is correspondingly greater. To minimize the associate risks of cell manufacturing adhering to international quality standards is critical. Current good tissue practice and current good manufacturing practice (cGMP) are examples of general standards that draw a baseline for clinical cell manufacturing. CGMP is a quality assurance system used mainly in the pharmaceutical industry. It ensures that the end product meets preset specifications. Despite each element is equally important, facility is the most obvious and tangible aspect of cGMP. Within the facility are product receipt area, quarantine area, material storage rooms, testing and quality control laboratories and the aseptic area where actual cell manipulation and tissue engineering will take place. The controlled environment of a carefully designed, constructed, validated, and maintained clean room will minimize the risks of environmental contamination during aseptic processing and decrease the possibility of cross-contamination. In 2007 with agreement of TUMS, Brain and Spinal Cord Injury Research Center (BASIR) was established to conduct translational and clinical researches in field of cell transplantation and tissue engineering for repairing brain and spinal injuries. To achieve higher level of safety for cell and tissue based products, we decided to build a cleanroom facility based on international standards. In this presentation we describe the experience of establishing a cGMP facility in an academic center, with paying particular attention to facility design, c

关键词： Cleanroom Clinical grade Cell therapy cGMP

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Co-transplantation of OECs and SCs combining treadmill training promote functional recovery from rat with spinal cord contused

Co-transplantation of OECs and SCs combining treadmill train...

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Tiansheng Sun Chaoqun Ye Zhi Liu Zhicheng Zhang Jun Wu Hongyun Huang Orthopaedic Department The Beijing Army General Hospital Beijing Hongtianji Neuroscience Academy

Due to the complex lesion and dificulty of axons regeneration after spinal cord injury, combined strategy has been focused at present. This study investigated the ability of co-transplantation of Schwann cells (SCs) and olfactory ensheathing cells(OECs) combining treadmill training to facilitate the neuronal plasticity and promote hind limb function recovery of subacute moderate, thoracic (T10) contusion in the rat. The treadmill training began post-injury day 7 for treadmill training rats that lasted for 20±10min per day, and 5 days per week for 10 weeks. Co-transplantation of OECs and SCs was performed two weeks after injury, with OEC injection into the adjacent spinal tissue and SC into the contusion lesion. At post-injury 11 weeks, GFP+-OECs were found migrating longitudinally and laterally from the injection site to injury site in gray matter and white matter, and some by central canal or pia from the injection entry site, While SCs remain largely concentrated at the transplanted site and were densely packed together. The transplanted SCs formed a graft within the injury site supporting the ingrowth of numerous, densely neurofilament-positive, MBP+ axons, and OECs supported moderate NF+, GAP-43+ axons, and a few MBP+ axons prolonging parallel to OECs processes. Treadmill training increased TH immunoreactive neuron in laminae VII and X of L2 cord. Co-transplantation of OECs and SCs combining treadmill training group displayed an increase of NF+ axons in lesion site and TH immunoreactive neuron in laminae Ⅶ and Ⅹ in L2 cord. Moreover, Co-transplantation of OECs and SCs significantly increased the BBB score at 5 and 8 weeks post-injury, while treadmill training at 5 and 11 weeks. Co-transplantation of OECs and SCs combining treadmill training resulted in the highest BBB score at 4 and 11 weeks. The current study details the differential neuronal plasticity : axons growth and remyline induced by co-transplantation of OECs and SCs, and neuron plasticity below lesion

关键词： Olfactory ensheathing glia cells Schwann cells co-transplantation, treadmill training Spinal cord Contusion neuronal plasticity functional recovery

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Calcium and vitamin D in spinal cord injury

Calcium and vitamin D in spinal cord injury

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第五届国际神经修复学会年会&全球神经保护和再生学会第九届年会并第四届国际脊髓损伤治疗与临床试验交流会

作者： Abbas Noroozi Javidan Hadis Sabour Farzad Shidfar Zahra Khazaeipour Fatemeh Shahbazi Mohammad reza Vafa Abbas Rahimi Hassan Emami Razavi Brain and Spinal Injury Repair (BASIR) Research Center Imam Khomeini Hospital ComplexTehran University of Medical SciencesTehranIran Ph.D Brain and Spinal Injury Repair (BASIR) Research Center Imam Khomeini Hospital ComplexTehran University of Medical SciencesTehranIranM.D.Ph.D Nutrition Department School of Public HealthTehran University of Medical SciencesTehranIranPh.D Brain and Spinal Injury Repair (BASIR) Research Center Research Deputy of Imam Khomeini Hospital ComplexTehran University of Medical SciencesTehranIranM.D.Ph.D Research Deputy of Imam Khomeini Hospital Complex Tehran University of Medical Sciences TehranIranPh.D Nutrition Department School of Public HealthTehran University of Medical SciencesTehranIranPh.D Statistics Department School of public HealthTehran University of Medical SciencesTehranIranPh.D Brain and Spinal Injury Repair Research Center Imam Khomeini HospitalTehran University of Medical SciencesTehranIranM.D

Objective: In patients with spinal cord injury, one of the factors that have a major role in the skeletal bone mass and bone health is nutrition. The two necessary nutrients for bone health are calcium and vitamin D. Dairy products and animal sources have been identified as the main sources for calcium and vitamin D in the diet. This study tries to compare serum 25-hydroxyl vitamin D levels and calcium with dietary intakes of chronic spinal cord injury persons. Method: 162 participants, 18-62 years of age, apparently healthy patients with a spinal cord injury were recruited in a cross-sectional study. Their dietary intakes were assessed by a semi quantitative food-frequency questionnaire. Serum calcium, phosphorous and vitamin D were measured. Results: In four different SCI patients group, the percentages of calcium, vitamin D and dairy intake except phosphorous were below guideline reference and were not age or sex dependent. The average values of serum total calcium in age < 40, was significantly more than age ≥ 40. A high percentage (43.6 %) of vitamin D deficiency was defined for the study patients. Conclusion: Conclusions: Calcium and vitamin D deficiency has been identified in spinal cord injury patients. Adequate sunlight exposure plus supplementation, fortified dairy intake for long periods, correction of nutritional deficits, and nutritional patient education is highly recommended.

关键词： Calcium , Vitamin D, Intake, Spinal Cord Injury

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