Twenty bacterial artificial chromosome (BAC) clones that could produce bright signals and no or very low fluorescence in situ hybridization (FISH) background were identified from Gossypium arboreum cv,JLZM,and Gossypi...
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Twenty bacterial artificial chromosome (BAC) clones that could produce bright signals and no or very low fluorescence in situ hybridization (FISH) background were identified from Gossypium arboreum cv,JLZM,and Gossypium hirsutum ***-1 and *** with 45S and 5S rDNA,a 22-prohe cocktail that could identify all the 13 G. arboreum chromosomes simultaneously was *** to their homology with tetraploid cotton,the *** chromosomes were designated as A1-A13,and a standard karyotype analyzing for G,arboreum was *** results demonstrated an application for multiple BAC-FISH in cotton cytogenetic studies,and a technique to overcome the problem of chromosome simultaneous recognition in mitotic cotton cells.
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