Sea-island cotton (Gossypium barbadense L.) has superior fiber quality properties such as length,fine- ness and strength,while upland cotton (Gossypium hirsutum L.) is characterized by high *** extensive studies on co...
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Sea-island cotton (Gossypium barbadense L.) has superior fiber quality properties such as length,fine- ness and strength,while upland cotton (Gossypium hirsutum L.) is characterized by high *** extensive studies on cotton fiber development,little is known about the cellular and molecular events responsible for cotton fiber elongation and second cell wall synthesis of these two *** technology offers a powerful tool to identify differentially expressed genes at different stages of *** results can be used to deepen our understanding of the molecular mechanisms of cotton fiber *** patterns of a subset of genes during fiber cell elongation and in second wall deposits were established to reveal features of upland cotton and Sea-island cotton fiber cells at the elongation stage. Comparative analysis of transcriptnmes by cDNA microarray showed 501 genes were differentially expressed at the fiber cell elongation *** these,321 genes were up-regulated and 172 genes were down-regulated in TM-1 versus Hai7124,re- *** additional genes were both up-regulated and down-regulated in TM-1 versus Hai7124 during distinctive developmental *** of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the genes differentially expressed indicated the encoding proteins were primarily involved in calcium ion/calmod- ulin binding,cell wall-related enzymes and the response to phytohormone stimulus,or found in signalling ***- tionally,sucrose and starch metabolism,flavonnid biosynthesis,phenylalanine metabolism,ascorbate and aldarate metabo- lism,pyrimidine metabolism and the phosphatidylinositol signalling system were involved in the fiber elongatinn stage.U- sing microarray technology,many novel genes were discovered and a large number of genes differentially expressed during fiber cell elongation were identified between *** ***-1 and *** ***
采用茶陵野生稻、东乡野生稻、小粒野生稻3个野生稻品种进行抗性候选基因转化与克隆研究。选用 9对引物分别对东乡野生稻、小粒野生稻与茶陵野生稻的总 DNA 进行了引物扩增筛选,结果表明第5对(WR-1、 WF-1)和第9对(S2、AS3)引物的扩增...
详细信息
采用茶陵野生稻、东乡野生稻、小粒野生稻3个野生稻品种进行抗性候选基因转化与克隆研究。选用 9对引物分别对东乡野生稻、小粒野生稻与茶陵野生稻的总 DNA 进行了引物扩增筛选,结果表明第5对(WR-1、 WF-1)和第9对(S2、AS3)引物的扩增效果最好,主要表现为带型最清楚,非特异性扩增最少。通过1μl(12.5 μmol)、2μl(25 μmol)、4μl(50 μmol)、8μl(100 μmol)和16μl(200 μmol)5种不同简并引物浓度的试验结果表明,16μl(200μmol)引物浓度的扩增效果最好,其电泳带型最清楚,非特异性的扩增也最少,符合简并引物合成原则,即简并性好且简并性不高。用第5对引物(WR-1、WF-1)对茶陵野生稻总 DNA 进行扩增、回收、克隆及测序分析,初步得到4类不同的抗性候选基因材料;用第9对引物对东乡野生稻总 DNA 进行扩增、回收、克隆,得到34个阳性重组子。
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