Macrophage migration inhibitory factor (MIF) is a key proinflammatory cytokine in immuno-inflammatory *** the first time,we examined the expression of MIF in Epinephelus awoara (***).MIF expressions have been detected...
Macrophage migration inhibitory factor (MIF) is a key proinflammatory cytokine in immuno-inflammatory *** the first time,we examined the expression of MIF in Epinephelus awoara (***).MIF expressions have been detected in head kidney,spleen,liver,brain,intestine,gill,heart,stomach,and muscle of *** infected with *** mRNA levels observed in infected groupers were higher than those in healthy ***,TNF-α,and IL-1 tissue levels have been measured by ELISA.A significant increase in MIF,TNF-α,and IL-1 tissue levels have been found in the treatment groups compared with those in ***,TNF-α and IL-1 tissue levels in spleen,head kidney,intestine,and liver of *** during the challenge trial with *** were significantly higher than those in controls *** was evidence that functions of MIF in a positive feedback loop with TNF-α,and IL-1 that could perpetuate the inflammatory process in grouper infected with *** conclusion,these results indicated that MIF was related to pathogen-induced immune response.
In this study,we constructed a protein-protein interaction (PPI) network of *** strain WX-02 with interolog method and domain-based method,which contained 15,864 edges and 2,448 *** computationally predicted networks ...
In this study,we constructed a protein-protein interaction (PPI) network of *** strain WX-02 with interolog method and domain-based method,which contained 15,864 edges and 2,448 *** computationally predicted networks have relatively low coverage and high false-positive rate,our prediction was confirmed from three perspectives: local structural features,functional similarities and transcriptional *** analysis of the COG heat map showed that protein interactions in *** WX-02 mainly occurred in the same functional *** incorporating the transcriptome data,we found that the topological properties of the PPI network were robust under normal and high salt *** addition,267 different protein complexes were identified and 117 poorly characterized proteins were annotated with certain functions based on the PPI ***,the sub-network showed that a hub protein CcpA jointed directly or indirectly many proteins related to γ-PGA synthesis and regulation,such as PgsB,GltA,GltB,ProB,Pro J,YcgM and two signal transduction systems ComP-ComA and ***,CcpA might play an important role in the regulation of γ-PGA *** study therefore will facilitate the understanding of the complex cellular behaviors and mechanisms of γ-PGA synthesis in *** WX-02.
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