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检索条件"任意字段=第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议"

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Cultured astrocytes from Wistar rats are more susceptible to swelling induced by glutamate than those from Sprague-Dawley rats

Cultured astrocytes from Wistar rats are more susceptible to...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Zhong-Fang Shi Li-Xin Xu Li-Ping Dong Xu Yan Shao-Hua Yang Fang Yuan Department of Pathophysiology Beijing Neurosurgical InstituteBeijing Tiantan HospitalCapital Medical University Department of Pharmacology and Neuroscience University of North Texas Health Science Center

Glutamate is one of the most important excitatory amino acids in the central nervous system, and plays important role in the formation and development of brain edema. Our previous studies have found that glutamate induced astrocyte swelling and increase in aquaporin 4(AQP4) expression in culture. Several studies have reported differences in the extent of ischemic lesion volumes existed among rat strains, but it is not clear whether cultured astrocytes from different rat strains have different response to glutamate. The aim of this study is to explore the difference between cultured astrocyte from Wistar rats and Sprague-Dawley rats after glutamate treament. Primary cultured astrocytes derived from the cerebral cortex of one-day-old Wistar or Sprague-Dawley rats. Subcultured astrocytes were treated with 0, 1 or 10 m M glutamate for 48 h. The viability of astrocytes was determined by LDH kits to assess the cellular injury, and the perimeter of astrocytes was measured by Image Pro Plus software after glial fibrillary acidic protein(GFAP) immunofluorescence staining to evaluate the astrocyte swelling. Then, the expression of AQP4 in cultured astrocytes was detected by quantitative reverse transcription polymerase chain reaction. No difference was found in the LDH release after the treatment of 0, 1 or 10 m M glutamate in cultured astrocytes from these two strains, suggesting that glutamate had no effect on astrocytes viability in culture from different rat strains. The perimeter of astrocytes from Wistar rats was normally shorter than that from Sprague-Dawley rats, butwas longer after the treatment of glutamate, suggesting that the change degree of astrocytes swelling induced by glutamate in Wistar rats was more significant than that from Sprague-Dawley rats. Meanwhile, AQP4 expression in Wistar rats was significantly higher than that from Sprague-Dawley rats after the incubation of 1 m M glutamate, suggesting that the change of AQP4 expression induced by glutamate in W

关键词： glutamate astrocyte GFAP LDH release,aquaporin 4

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Orexin-B suppresses GABA currents through a PLC/PKC signaling pathway in rat retinal bipolar cells

Orexin-B suppresses GABA currents through a PLC/PKC signalin...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Gong Zhang Shi-Jun Weng Xiong-Li Yang Yong-Mei Zhong Institute of Neurobiology Institutes of Brain ScienceState Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain ScienceFudan University

Objective Two neuropeptides, orexin-A and orexin-B, have been implicated in the modulation of neuronal firing, neurotransmitter release, sleep/wake, feeding behaviors by activating two orphan G-protein-coupled receptors: orexin 1 and orexin 2 receptors(OX1R and OX2R). However, the function of these peptides in the retina is still largelyunknown. In this work, we investigated whether and how orexin-B modulates GABA receptor-mediated currents in rod-dominant ON type bipolar cells(Rod-ON-BCs). Methods The expression of OXRs in rat Rod-ON-BCs was identified by immunocytochemistry. Whole cell patch-clamp recording techniques were used to investigate the effect of orexin-B on GABA currents in freshly dissociated Rod-ON-BCs. Results Double immunofluorescence experiments conducted in isolated cells showed that OX1 R and OX2 R were expressed in somata, dendrites and axon terminals of rat Rod-ON-BCs. Using whole-cell patch-clamp recording, we demonstrated that orexin-B suppressed GABA currents in Rod-ON-BCs in a dose-dependent manner. Orexin-B suppressed GABAC receptor-, but not GABAA receptor-mediated currents of the BCs. Consistent with the immunocytochemical results, the orexin-B effect was partly eliminated either by SB334867, a specific OX1 R antagonist, or TCS OX2 29, a specific OX2 R antagonist. Moreover, TCS1102, a dual orexin receptor antagonist, completely eliminated the orexin-B-induced suppression of GABA currents. The orexin-B effect was abolished by intracellular dialysis of GDP-β-S or preincubation with the Gi/o inhibitor pertussis toxin, suggesting the involvement of Gi/o proteins. In addition, intracellular dialysis of either the phosphatidylinositol(PI)-phospholipase(PLC) inhibitor U73122 or the phosphatidylcholine(PC)-PLC inhibitor D609, orexin-B persisted to suppress the GABA currents of Rod-ON-BCs. However, intracellular infusion of both U73122 and D609, oreixn-B did not change the GABA currents. Furthermore, the orexin-B effect was eliminated by the prot

关键词： orexin-B orexin receptors GABA receptor bipolar cells retina

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Remote ischemic postconditioning enhances arteriogenesis and attenuates cerebral damage for ischemic stroke in mice

Remote ischemic postconditioning enhances arteriogenesis and...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Ying Zhang Jinhuan Gao Kaiyin Liu Changhong Ren Yuchuan Ding Junfa Li Xunming Ji Cesar V.Borlongan Department of Neurobiology and Beijing Institute for Brain Disorders Capital Medical University Institute of Hypoxia Medicine Xuanwu HospitalCapital Medical University Department of Neurosurgery Wayne State University School of Medicine Department of Neurosurgery and Brain Repair University of South Florida

As a therapeutic strategy, remote ischemic postconditioning holds great promise for ischemic stroke, but the underlying mechanisms yet remain unclear, hindering its translation from bench to beside. Here, we will specifically explore the role of remote ischemic conditioning in therapeutic mobilization of spleen monocytes/macrophages(MMs) for brain collateral artery growth after ischemic stroke in mice. Focal cerebral ischemia was generated by occlusion of distal middle cerebral artery on adult C57BL/6J male mice. Then the mice were randomly divided into sham-operated group, model group, remote ischemic postconditioning(RIPC) group(bilateral lower limb by occluding and releasing the femoral artery for three cycles), or granulocyte colony-stimulating factor(G-CSF) group(100mg/kg, sc.). Twenty four hours after brain ischemic injury, results showed that:(1) while RIPC, and G-CSF injection, compared to no treatment significantly reduced infarct volume(37.4% and 29.3% vs 45.1%) and neurological deficit score(-0.2 and-0.1 vs-0.7), the greatest neuroprotection was found in stroke animals that received G-CSF(p's<0.05).(2) Both RIPC and G-CSF enhanced the leptomeningeal collateral growth compared to model group.(3) A single dose of 5-fluorouracil(5-FU) administration(150mg/kg intraperitoneally) severely depleted blood monocytes at 4 days, and this intervention significantly interfered with growth response of leptomeningeal anastomoses by RIPC or G-CSF treatment.(4) The protein expression of pro-angiogenic factors such as Ang-2 were increased by ischemia. Treatment with RIPC or G-CSF both further upregulated the expression of angiogenic factors compared to model group. In conclusion, remote limb ischemic postconditionin enhances collateral artery growth and reduces brain injury in a mouse model of ischemic stroke, similarly to G-CSF. Further molecular studies are warranted to define the mechanisms of recruitment of MMs from spleen to brain, and the MMs' following role for neur

关键词： remote ischemic postconditioning(RIPC) ischemic stroke vascular remodeling arteriogenesis monocytes/macraphages(MMs)

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The Influence of Substantia Nigra Neurons and Akt through Acupuncture of Chorea-tremble Controlled Area Combined with Madopar in PD

The Influence of Substantia Nigra Neurons and Akt through Ac...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Yuan-Yuan Wang Hong-Mei Sun Jing Feng Xiao-Gang Gong Yu-Shan Gao Shu-Jing Zhang Hai-Xia Wu Cong Gai Xin He Zhen-Yu Guo Department of Anatomy Beijing University of Chinese Medicine

Objective To study the protective effect of acupuncture of Chorea-tremble controlled area combined with madopar on the dopaminergic neurons in Parkinson's disease(PD) and the possible mechanism of treating Parkinson's disease via observation of Akt expression. Methods Male C57BL/6 mice were randomly divided into five groups: normal control group, model group, acupuncture group, madopar group, acupuncture combined with madopar group. The animals were killed on the 28 th day after intraperitoneal injection of MPTP for the first time. The changes of behavior of rats were detected by climbing rod method. The changes of dopaminergic neurons express in the sustantia nigra and the Akt expression in brain were measured by immunohistochemistry. Results The pole-climbing time in three therapy groups had obvious difference compared with model group(P<0.05), behavior improved significantly. The express of dopaminergic neurons was less in four other groups than in normal control group(P<0.05), while the express of acupuncture mixed with madopar group was more than model group and madopar group notably(P<0.05). The result showed that in the area of substantia nigra, the Akt expression in the needling combined with madopar group was higher than other four groups(P<0.05). In the CA1 hippocampus area, the expression of Akt in the model group, needling group and needling combined with medopar group obviously decreased compared with nomal group(P<0.05);the expression of Akt in the needling combined with medopar group increased compared with model group and medopar group in CA1, CA3 and CA4 area(P<0.05);compared with needling group, the density of Akt in needling combined withmedopar group increased in CA1 and CA4 area(P<0.05). Conclusions Acupuncture of Chorea-tremble controlled area especially combined with madopar has a neuroprotective effect on dopaminergic neurons in substania nigra of PD. The mechanism is probably related to increasing the level of Akt expression in brain.

关键词： Parkinson's disease 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Chorea-tremble controlled area madopar protein kinase B

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AMPA Receptor Signaling Pathway Was Involved in the Antidepressant Effects of Lentinan in Mice

AMPA Receptor Signaling Pathway Was Involved in the Antidepr...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Lijuan Sun Hongkun Bao Pengzhan Ran Ming Zhu Bai Li Yangyang Hou Jun Nie Chunjie Xiao Jing Du Yunnan University School of Medicine

Background: Depression is a common disease that affect millions of Chinese. Currently, there is an urgent demand for the safe, fast-acting and mechanistically novel antidepressant drugs. This study was designed to investigate the anti-depressant-like effects of lentinan(LNT) in mouse models of depression, and to explore the roles of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid(AMPA) receptors, as well as some neural immune inflammatory and anti-inflammatory cytokines in the antidepressant effects of lentinan. Methods: CD-1 mice(7-8 weeks old) were treated with positive control imipramine(15 mg/kg), lentinan low dose(5 mg/kg), lentinan intermediate dose(12.5 mg/kg), lentinan high dose(20 mg/kg), and saline. The tail suspension test(TST) was performed after one day of intraperitoneal injection, forced swimming test(FST) was tested at day 3, and open-field test was determined at day 5. After open-field test, the different brain regions, such as prefrontal cortex, hippocampus, and striatum, were collected. The levels of the AMPA receptor subunit Glu R1 and Glu R1(S845) phosphorylation were then determined by Western Blot analysis in mouse prefrontal cortex. In addition, anti-inflammatory cytokine IL-4 was also detected by Western Blot analysis. Results:(1) The treatment of lentinan with intermediate dose and the high dose significantly reduced the immobility time in the TST and FST in mice after one day and three days of treatment.(2) In the open field test, the 3 different doses of lentinan treatment did not significantly change the total distance traveled compared to the negative control group.(3)Western blot analysis showed that Glu R1(S845) phosphorylation was enhanced after lentinan treatment for 5 days. The expression level of Glu R1 had no significant difference(P > 0.05). In addition, the expression levels of the anti-inflammatory cytokine IL-4 were significantly increased after lentinan treatment for 5 days(P < 0.05). Conclusions: Lentinan treatmen

关键词： Depression lentinan AMPA receptor GluR1(S845) phosphorylation IL-4

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Normobaric hyperoxia slows BBB damage and expands the therapeutic time window for tPA treatment in cerebral ischemia

Normobaric hyperoxia slows BBB damage and expands the therap...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Jia Liang Zhifeng Qi Wenlan Liu Peng Wang Wenjuan Shi Wen Dong Xunming Ji Yumin Luo KeJian Liu Cerebrovascular Diseases Research Institute Xuanwu Hospital of Capital Medical University Central Laboratory of Liaoning Medical University Department of Pharmaceutical Sciences University of New Mexico

Background and purpose: Prolonged ischemia causes blood-brain barrier(BBB) damage and increases the incidence of neurovasculature complications secondary to reperfusion. Therefore, targeting ischemic BBB damage pathogenesis is critical to reducing neurovasculature complications and expanding the therapeutic time window of tissue-type plasminogen activator(t PA) thrombolysis. This study investigates whether increasing cerebral tissue p O2 through normobaric hyperoxia(NBO) treatment will slow the progression of BBB damage and thus improve the outcome of delayed t PA treatment after cerebral ischemia. Methods: Rats were exposed to NBO(100%O2) or normoxia(21%O2) during 3, 5, or 7-h middle cerebral artery occlusion. Fifteen min before reperfusion, t PA was continuously infused to rats over 30 min. Neurological score, mortality rate, and BBB permeability were determined. MMP-9 was measured by gelatin zymography, and tight junction proteins(occludin and cluadin-5) by Western blot in the isolated cerebral microvessels. Results: NBO slowed the progression of ischemic BBB damage pathogenesis, evidenced by reduced Evan’s blue leakage, smaller edema and hemorrhagic volume in NBO-treated rats. NBO treatment reduced MMP-9 induction and the loss of tight junction proteins in ischemic cerebral microvessels. NBO-afforded BBB protection was maintained during t PA reperfusion, resulting in improved neurological functions, significant reductions in brain edema, hemorrhagic volume and mortality rate, even when t PA was given after prolonged ischemia(7h). Conclusions: Early NBO treatment slows ischemic BBB damage pathogenesis and significantly improves the outcome of delayed t PA treatment, providing new evidence supporting NBO as an effective adjunctive therapy to extend the time window of t PA thrombolysis for ischemic stroke.

关键词： blood-brain barrier ischemia matrix metalloproteinase-9 normobaric hyperoxia tissue-type plasminogen activator（tPA）

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MiRNA-200b regulates RMP7-induced an increase in blood-tumor barrier permeability by targeting RhoA and ROCKⅡ

MiRNA-200b regulates RMP7-induced an increase in blood-tumor...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Teng Ma Yi-xue Xue Department of Neurobiology College of Basic MedicineChina Medical University Institute of Pathology and Pathophysiology China Medical University

The purposes of this study were to investigate the potential roles of mi R-200 b in regulating RMP7-induced an increase in blood-tumor barrier(BTB) permeability and its possible molecular mechanisms. Micro RNAs(mi Rs) are short non-coding transcripts that regulate gene expression by binding to target m RNAs, preventing their expression. We conducted an expression analysis of mi RNAs and several of their target m RNAs, by using microarrays and quantitative Reverse Transcription-Polymerase Chain Reaction(RT-PCR), in blood-tumor barrier(BTB) by RMP7, as compared to BTB and normal blood-brain barrier(BBB), in order to identify candidate mi RNAs involved in TJ disassembly and an increase inBTB permeability. Microarray, together with confirmatory RT-qP CR analyses, showed 18 significantly deregulated mi RNAs including miR-200 b in BTB, and 44 significantly upregulated mi RNAs in BTB. While, as expected, some mi RNAs have been previously reported to be associated with TJ disassembly, including mi R-181 a, mi R-34 a and mi R-34 c, others were new. Similarly, there were 26 significantly deregulated mi RNAs and 8 significantly upregulated mi RNAs in BTB by RMP7 versus BTB comparison. The impact of these changes in mi R expression on target genes is suggested by the associated deregulation of the genes which contribute to TJ disassembly and an increase in BTB permeability. RMP7 could induce tight junction(TJ) opening of BTB, which resulted in an increase in BTB permeability. RMP7 resulted in a decrease in mi R-200 b expression in human cerebral microvascular endothelial cells line h CMEC/D3 of BTB. Overexpression of mi R-200 b and mi R-200 b sliencing were established by transfecting mi R-200 b mimics and inhibitors into h CMEC/D3 respectively. Overexpression of mi R-200 b could inhibit endothelial leakage and restored normal transendothelial electric resistance(TEER) values in BTB. Simultaneous, a shift in occludin and claudin-5 distribution from insoluble to soluble fraction

关键词： microarray miR-200b RMP7 RhoA ROCKⅡ BTB

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Ultra-rapid generation of genome-edited mice crucial for neurodevelopmental research via CRISPR/Cas9

Ultra-rapid generation of genome-edited mice crucial for neu...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Yukiko U.Inoue Kou Hiraga Takayoshi Inoue Department of Biochemistry and Cellular Biology National Institute of NeuroscienceNational Center of Neurology and Psychiatry

Gene knockout mice and gene knockin mice are pivotal tools for studying higher-order life phenomenon in vivo, and also important for drug discovery as disease models. However, traditional gene targeting in ES cells is complex, time-consuming, and expensive process. The production of genetically modified mice has been dramatically accelerated by the recent fast-growing genome editing technologies using direct injection of CRISPR/Cas9 DNA or RNA into fertilized eggs, generating DNA double-strand breaks at specific sequences leading to targeted mutations. Coinjection of DNA template containing homology to the sequences flanking the double-strand break can produce mutant alleles with DNA insertion. Futhermore, multiple gene knockout mice can also be easily generated by a single injection of multiple guide RNAs without laborious crossing. Here, we targeted neurodevelopmentally crucial genes such as Pax6 and cadherins. Pax6 encodes a key transcription factor that plays maser roles in eye development, early brain patterning, cortical arealization and so on. By injecting the p X330-sgR NA plasmid DNA into one-cell embryos, we achieved Pax6 knockout mice quickly and effectively(more than 75% embryos showed eyeless, shorter nose, and no olfactory bulb phenotypes just like spontaneous Pax6 mutant) within one mouth. Furthermore, by injecting sgR NA, Cas9 m RNA, and the targeting vector containing ires-EGFP gene casette, we could rapidly generate a Pax6 ires-EGFP knockin mouse recapitulating endogenous gene expression patterns within a few months. Cadherin genes encode cell adhesion molecules at synapsesand exert multiple functions in neurodevelopmental process, like axonal pathfinding, growth cone motility, and synapse formation. By injecting the mixture of p X330-sgR NA plasmid DNA for three cadherin genes, we're generating multiple gene knockout mice without mating. Although single knockout of cadherin genes showed limited effects on behavior because of the functional redunda

关键词： Genome editing CRISPR/Cas9 Pax6 cadherins

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The improve effect of salvianolic acids on cognitive function in mice after chronic stress and its mechanism

The improve effect of salvianolic acids on cognitive functio...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Bing Han Meiyu Sun Li Shen Qian Wang Yuan Geng Mingwei Wang Department of Neurology the First Hospital of Hebei Medical University Brain aging and cognitive neuroscience key laboratory of Hebei province Clinical laboratory the First Hospital of Hebei Medical University

Objective This study intends to establish a chronic restraint stress model to explore the potential neuroprotective effects of salvianolic acids against restraint stress-induced cognitive deficits and the mechanisms and the change of the expression of BDNF in the hippocampus. Methods The male mice were randomly divided into three groups(n = 14).Control group without stress treatment and inject of saline daily(10 ml/kg). Chronic restraint stress(stress) group inject of saline daily(10 ml/kg), and restraint for 8 h. Chronic restraint stress plus salvianolic acids(stress+sal) group inject of salvonolic acids(10 ml/kg) daily, and restraint for 8 h. After 14 days,we weighed them, tested their learning andmemory capacity by novel object recognition test(NORT), detected the hippocampus BDNF m RNA and protein expression by real-time PCR and immunohistochemical method. Results The increment of body weight of control group was 8.01±2.12. Compared with control group, the stress(3.84±1.40) and stress+sal group(3.84±1.40) were significantly decreased(P < 0.01). NORT showed the mean time(s) of control group spent exploring the novel object was 19.64 ±2.04, that of the stress+sal group was 17.76±2.76. The stressed rats spent less time(11.86±2.42) exploring the novel objects, compared with that of the control group(P < 0.05) and stress+sal group(P < 0.05). We used real-time PCR method to detect the BDNF m RNA expression in hippocampus. The relative expression of target gene of control group was 0.56±0.13, while that of the stress+sal group was 0.55±0.19. The relative expression of target gene of stress group(0.32±0.11)was significantly lower than the control group(P < 0.05) and stress+sal group(P < 0.05). The number of immunopositive cells of BDNF in control group was 36.08±0.7 and that of the stress+sal group was 34.19±0.6. The number of immunopositive cells of BDNF in stress group(33.49±0.98) was significantly lower than the control group(P < 0.05) and stress+sal group(P < 0.05).

关键词： chronic restraint stress salvonolic acids NORT immunohistochemical realtime PCR

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Observations of neuropathic pain response,sensation and emotion in diabetic rats

Observations of neuropathic pain response,sensation and emot...

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第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

作者： Jia-Shen JIANG Jia YUAN Yao-Jun SHAO Ju LIU Yu-Chen FU Ting-Ting ZHOU Chao ZHENG Meng-Ya WANG Cell Electrophysiology Laboratory Wannan Medical College Rising-Star Group Wannan Medical College

Objective To explore the possible psychophysiological mechanisms underlying diabetic neuropathic pain by observing the changes of the pain response, sensation and emotion in diabetic rats. Methods Twenty adult male SD rats were evenly divided into 2 groups(control and diabetic model group), and the diabetic model rats were prepared by injection of streptosotocinz(STZ). Light radiant heat method was used to test tail-flick latency of rats. Electrical activities of dysgranular zone(DZ), the pain sensation-related brain region, and basolateral amygdala(BLA), the pain emotion-related nucleus, were both extracellularly recorded with simultaneous observation of electrocardiograph, electromyography of breath muscles, and body temperature in anaesthetized rats. Thermal stimulation and tail pinch were used to elicit nociceptive responses. Results(1) The blood glucose level of model group rats was maintained over 16 mmol/L after the injection of STZ.(2) Tail-flick latency of model group rats was significantly shorter than that of control group(n=9, P<0.01).(3) The baseline of BLA firing rate of model group rats was increased significantly compared with that of control group(n=9, P<0.01). The firing rate of BLA and DZ both increased in two groups during 2 kinds of stimulation(P<0.05). During the thermal stimulation, the increases of both BLA and DZ of model group were higher than those of control group(P<0.05), while during the tail pinch, only the increase of BLA was higher than that of control group(P<0.05).(4)The heart and respiratory rate increased in 2 groups during thermal and tail pinch stimulation, and both of them recovered quickly(P<0.05), while those of model group did not recover(P<0.01). Conclusion In diabetic rats, not only the pain response is sensitized, but also the pain sensation, pain emotion and physiological responses induced by nociceptive stimuli are enhanced significantly.

关键词： diabetic neuropathic pain pain response pain sensation pain emotion

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