Background: Many pre-miRNAs have been reported to yield two kinds of mature miRNAs, although the two products, miR-#-5p and miR-#-3p, may vary in expression *** selection and/or arm switching in miRNAs may influence m...
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Background: Many pre-miRNAs have been reported to yield two kinds of mature miRNAs, although the two products, miR-#-5p and miR-#-3p, may vary in expression *** selection and/or arm switching in miRNAs may influence miRNA expression ***: In the present study, we mainly discussed this under three aspects: ① primary evolutionary analysis of human miRNAs;② evolutionary analysis of miRNAs from different arms across the typical vertebrates;③ expression pattern analysis of miRNAs at the miRNA/isomiR levels using public small RNA sequencing datasets.
Background: Solid pseudopapillary neoplasm is a distinctive pancreatic tumor of low-malignant potential due to its characteristic clinicopathologic features and distinct *** solid pseudopapillary neoplasm related prot...
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Background: Solid pseudopapillary neoplasm is a distinctive pancreatic tumor of low-malignant potential due to its characteristic clinicopathologic features and distinct *** solid pseudopapillary neoplasm related protein coding genes have been increasingly exposed, an integrated analysis for the role of miRNA and mRNA in it has not been widely investigated.A central goal of our study is to uncover regulatory mechanisms underlying oncogenesis of solid pseudopapillary neoplasm and potential therapeutic applications by constructing the gene regulatory network among TFs, microRNAs and their target genes.
Background: Antimicrobial peptides (AMPs) as evolutionarily conserved components of innate immune system protect against pathogens of bacteria, fungi, viruses, tumors and ***, AMPs have major effects on inflammation i...
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Background: Antimicrobial peptides (AMPs) as evolutionarily conserved components of innate immune system protect against pathogens of bacteria, fungi, viruses, tumors and ***, AMPs have major effects on inflammation in mammals by influencing diverse cellular processes containing cytokine release, chemotaxis, antigen presentation, angiogenesis and wound *** the problem of antibiotics resistance is growing, AMPs are considered to be the promising substitute for *** far, more than 2400 mature and active antimicrobial peptides of different origins have been identified and some have entered clinical *** a series of databases dedicated to AMPs in general or specific sources have been established to store data and provide functions of query, statistics, prediction, sequence alignment and design.
Background: Many proteins can change their conformations or interact with different partners to show different biological *** it's difficult and expensive for experiments to verify which functions may be observed ...
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Background: Many proteins can change their conformations or interact with different partners to show different biological *** it's difficult and expensive for experiments to verify which functions may be observed for a *** is believed that protein functions relate closely with their 3D *** it is possible to find new functions for a protein by comparing its 3D structure to all the other know protein 3D *** the structures of two low-homologous proteins are shown to be similar, the functions of them will be similar *** the traditional 3D structure methods have many drawbacks.
Background: Population structure (PS), including population stratification and admixture, is a significant confounder in genome-wide association studies (GWAS) as it may produce spurious *** forest (RF) has been incre...
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Background: Population structure (PS), including population stratification and admixture, is a significant confounder in genome-wide association studies (GWAS) as it may produce spurious *** forest (RF) has been increasingly applied in GWAS data analysis because of its advantage in analyzing high dimensional genetic *** creates importance measures for SNPs which are helpful for feature ***, if population structure is not appropriately corrected, RF tends to give high importance to disease-unrelated SNPs with different frequencies of allele or genotype among subpopulations, leading to inaccurate results.
Background: Cancer biomarker plays an important role in detecting cancer as well as the mechanism of cancer occurrence and *** the development of transcriptomics research, especially the widely used high-throughput mi...
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Background: Cancer biomarker plays an important role in detecting cancer as well as the mechanism of cancer occurrence and *** the development of transcriptomics research, especially the widely used high-throughput microarray chip and RNA sequencing technology, researchers have obtained a large number of tumor related data and information on he transcriptional *** such a mass of data and information, how to identify clinical biomarkers systematically and efficiently has become one of advanced research projects of cancer biomarker research.
Background: The chromosome conformation capture (3C) derived techniques, such as 4C, 5C, ChIA-PET and Hi-C, have revealed that the chromatin is organized in a hierarchical way in three-dimensional (3D) space to provid...
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Background: The chromosome conformation capture (3C) derived techniques, such as 4C, 5C, ChIA-PET and Hi-C, have revealed that the chromatin is organized in a hierarchical way in three-dimensional (3D) space to provide another layer of chromatin *** it is important to develop computational frameworks to analyze and reconstruct the chromatin 3D *** we present an approach to identify topological domains and boundaries from Hi-C chromatin interactions.A newly developed chromatin 3D modeling method, called AutoChrom3D, can be used to reconstruct the 3D structures of selected chromatin regions.
Background: The abnormally methylated DNA can lead to carcinogenesis and some *** methylation, which mostly occurs at CpG sites, is one of epigenetic changes and plays a key role in regulatory gene expression.
Background: The abnormally methylated DNA can lead to carcinogenesis and some *** methylation, which mostly occurs at CpG sites, is one of epigenetic changes and plays a key role in regulatory gene expression.
Background: Allosteric drugs, taking an action-at-a-distance mechanism to achieve regulations on the protein functions, have several advantages over conventional orthosteric drugs, including diverse regulation types a...
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Background: Allosteric drugs, taking an action-at-a-distance mechanism to achieve regulations on the protein functions, have several advantages over conventional orthosteric drugs, including diverse regulation types and fewer side *** rational design of allosteric ligands, however, remains challenging, especially for the step of allosteric site *** allosteric ligand binding may work by inducing changes in the pattern of residue-residue interactions, we calculated the residue-residue interaction energies within the allosteric site based on the molecular mechanics generalized Born surface area (MM/GBSA) energy decomposition scheme.
Methods: Interspecies somatic cell nuclear transfer (iSCNT) has been regarded as a potential alternative in rescuing highly endangered species and can be used as a model for studying nuclear-cytoplasmic ***, iSCNT emb...
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Methods: Interspecies somatic cell nuclear transfer (iSCNT) has been regarded as a potential alternative in rescuing highly endangered species and can be used as a model for studying nuclear-cytoplasmic ***, iSCNT embryos often fail to produce viable *** alterations of normal molecular mechanisms contributing to extremely poor development is for the most part unknown.
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