Background: Although it has already been well documented that, in contrast to translation and transcription systems, DNA replication systems are not universally conserved among the three domains of life (Bacteria, Arc...
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Background: Although it has already been well documented that, in contrast to translation and transcription systems, DNA replication systems are not universally conserved among the three domains of life (Bacteria, Archaea, and Eukaryotes), it comes as a great surprise that even within bacteria the functional DNA polymerase Ⅲ alpha subunits (DnaE) have four different homologs, i.e., DnaE1, DnaE2, DnaE3, and PolC.
Background: Alternative splicing is pervasive in multi-exon genes of human and other organisms, and is a major contributor to the expansion of the transcriptome diversity and functional *** array data demonstrated tha...
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Background: Alternative splicing is pervasive in multi-exon genes of human and other organisms, and is a major contributor to the expansion of the transcriptome diversity and functional *** array data demonstrated that as much as 90% of human genes generate alternatively spliced products.
It has long been recognized that the "universal" 16S rRNA gene primers commonly used in microbial community surveys are inherently biased toward some *** our previous work, we found that genera have their ow...
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It has long been recognized that the "universal" 16S rRNA gene primers commonly used in microbial community surveys are inherently biased toward some *** our previous work, we found that genera have their own best genus-specific amplicons for genus assignment, which sometimes is not located in hyper variable regions (HVRs).
Background: The typical symptom of Parkinson's disease is the presence of α-synuclein aggregates in the form of β-structure that can be soluble or *** N-terminal region (residues 1-60) of α-synuclein plays a ke...
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Background: The typical symptom of Parkinson's disease is the presence of α-synuclein aggregates in the form of β-structure that can be soluble or *** N-terminal region (residues 1-60) of α-synuclein plays a key role in the formation of α-synuclein ***, the dimerization is important for α-synuclein conformational transition and ***, it is difficult to simulate 120 residues in explicit water *** this study, we selected N-terminal 12 residues peptide of α-synuclein (α-syn12).
Background: Microbial nanowires are conductive protein appendages which facilitate long-range extracellular electron transfer (EET).Different species of Geobacter and Shewanella have been found transfer electrons alon...
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Background: Microbial nanowires are conductive protein appendages which facilitate long-range extracellular electron transfer (EET).Different species of Geobacter and Shewanella have been found transfer electrons along their nanowires, which might be used for bioremediation of toxic metals or generation of electricity in microbial fuel ***, studies have suggested that these different species have different mechanisms for EET along their conductive *** sulfurreducens nanowires, which are comprised of pili, have been reported for its metal-like conductivity attributed to overlapping pi-pi orbitals of aromatic amino *** of the subunit structure which is determined with solution state NMR spectroscopy and a stepping stone model proposed before, the pilus structure of *** and the details of mechanism of electron transfer along the pili still remain unknown.
Background:In recent years, mass spectrometry-based shotgun proteomics has become the major method for large-scale protein ***, it's estimated that at least 70% spectra in MSdata cannot be successfully *** the sea...
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Background:In recent years, mass spectrometry-based shotgun proteomics has become the major method for large-scale protein ***, it's estimated that at least 70% spectra in MSdata cannot be successfully *** the search space is one of the most common ways to improve the efficiency for protein ***, the expanding database may not contribute to improve the accuracy and sensitivity of peptide *** alleviate this issue, we introduced the multi-level protein sequence database search strategy and proved this strategy can identify more results using a universal standard proteins set.
Background: The homologous FlgM proteins have varieties of disordered degree and temperature adaptability, and the disordered degree is related with high temperature *** is one of the strategies naturally for adaptati...
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Background: The homologous FlgM proteins have varieties of disordered degree and temperature adaptability, and the disordered degree is related with high temperature *** is one of the strategies naturally for adaptation to high temperature that the disordered degree is converted from a fully disordered state to a semidisordered *** thermophile aquifex aeolicus FlgM protein (AAFlgM) is intrinsically disordered proteins, and also semidisordered *** remains unknown how the conformation transitions along with temperature at atomic-level.
Background: Intrinsically disordered proteins (IDPs) are a kind of widespread protein that lack of stable three-dimensional structure, although they carry important biological *** of the flexible structure, it is diff...
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Background: Intrinsically disordered proteins (IDPs) are a kind of widespread protein that lack of stable three-dimensional structure, although they carry important biological *** of the flexible structure, it is difficult to experimentally study *** computational prediction of IDPs have been one of the most important topics of bioinformatics.
Determination of oligonucleotide binding sites is crucial for nucleotide hybridization based molecular biological technology, such as *** sequence similarity method such as BLAST was adopted by most software, it doesn...
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Determination of oligonucleotide binding sites is crucial for nucleotide hybridization based molecular biological technology, such as *** sequence similarity method such as BLAST was adopted by most software, it doesn't actually reflect the real hybrid states between oligonucleotide and its template sequence.
Background: With the advances of Next Generation Sequencing technology, the application of bioinformatics to solve genomics problem become a focus of recent *** accumulation of human genetic variation data make it fea...
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Background: With the advances of Next Generation Sequencing technology, the application of bioinformatics to solve genomics problem become a focus of recent *** accumulation of human genetic variation data make it feasible to utilize computational method to improve the analysis, such as using imputation to improve the performance in Whole Exome Sequencing (WES) analysis.
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