Background: The multiplexing becomes one of the major limitations of the next-generation sequencing platforms in application to low complexity samples, including the sequencing-by-ligation *** application of barcodes ...
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Background: The multiplexing becomes one of the major limitations of the next-generation sequencing platforms in application to low complexity samples, including the sequencing-by-ligation *** application of barcodes mitigates this limitation but becomes less practicable when the sample number is large.
Background: The PB1-F2 is the 11th protein of influenza A virus, encoded by the +1 alternate open reading frame (ORF) in the PB1 gene and previously reported to be a virulence factor that could contributed to the vira...
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Background: The PB1-F2 is the 11th protein of influenza A virus, encoded by the +1 alternate open reading frame (ORF) in the PB1 gene and previously reported to be a virulence factor that could contributed to the viral *** understand how the PB1-F2 protein evolved in different suptypes of influenza A viruses in different hosts, molecular characteristics of this protein were analyzed.
Background: Alternative splicing is a molecular mechanism that produces multiple proteins from a single gene, and is thought to produce in diversity of proteins translated from a limited number of ***, it is still unc...
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Background: Alternative splicing is a molecular mechanism that produces multiple proteins from a single gene, and is thought to produce in diversity of proteins translated from a limited number of ***, it is still unclear that what physicochemical factors in alternative splicing lead to difference of structure and to what extent alternative splicing influence protein stability.
Background: Irinotecan (SN38) and oxaliplatin-being part of FOLFIRI or XELOX/ FOLFOX treatment protocols, respectively-are commonly used in the treatment of colorectal cancer ***, the frequent development of resistanc...
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Background: Irinotecan (SN38) and oxaliplatin-being part of FOLFIRI or XELOX/ FOLFOX treatment protocols, respectively-are commonly used in the treatment of colorectal cancer ***, the frequent development of resistance to these drugs represents a considerable challenge in the clinic.
Background: It is imperative to develop new simulation sampling method to characterize *** et al divided the long sequence IDPs into eight residues long fragments (for α-synuclein, the first two fragments are residue...
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Background: It is imperative to develop new simulation sampling method to characterize *** et al divided the long sequence IDPs into eight residues long fragments (for α-synuclein, the first two fragments are residues 1-8 and 6-13), then used temperature replica-exchange molecular dynamics (T-REMD) simulation (using implicit solvent model) to construct the structural library of short segments, and then used bayesian statistics to determine a probability distribution for each conformation in the ensemble based on both theoretical and experimental information.
Background: A recombinant carboxylesterase, cloned from Pseudomonas putidaand designated as rPPE, is capable of catalyzing the bioresolution of racemic 2-acetoxy-2-(2'-chlorophenyl)acetate (rac-AcO-CPA) with excel...
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Background: A recombinant carboxylesterase, cloned from Pseudomonas putidaand designated as rPPE, is capable of catalyzing the bioresolution of racemic 2-acetoxy-2-(2'-chlorophenyl)acetate (rac-AcO-CPA) with excellent *** design of the enzyme shows that the variant W187H could increase the activity by ~100-fold comparedto the wild type (WT).As the crystal structures of Apo-rPPE and rPPE in complex with (S)-AcO-CPA were resolved recently, we can carry out molecular dynamic simulations to gain more insights into the molecular mechanism regarding ligand recognition and catalytic efficiency of the carboxylesterase rPPE.
Background: An important goal of synthetic biology is the rational design and predictable implementation of synthetic gene circuits using standardized and interchangeable ***, engineering of complex circuits in mammal...
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Background: An important goal of synthetic biology is the rational design and predictable implementation of synthetic gene circuits using standardized and interchangeable ***, engineering of complex circuits in mammalian cells is currently limited by the availability of well-characterized and orthogonal transcriptional repressors.
Background: Histone variant H2A.Z (Htz1 in yeast) substitution for H2A plays important roles in diverse DNA *** and evicting Htz1 from chromatin requires the coordination of the SWR1 remodeling complex, specific histo...
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Background: Histone variant H2A.Z (Htz1 in yeast) substitution for H2A plays important roles in diverse DNA *** and evicting Htz1 from chromatin requires the coordination of the SWR1 remodeling complex, specific histone modifications, and histone *** chaperones Chz1 and Nap1 are important for the deposition Htz1 into *** is an Htz1-H2B-specific chaperone that delivers histone Htz1 that substitutes for ***1 can bind (H3-H4)2 tetramers, H2A-H2B dimers, and H2A.Z-H2B ***16 functions in transcription elongation and also possesses a histone chaperone ***, the exact roles of these chaperones in assembling Htz1-containing nucleosome remain largely unknown.
Background: Helicobacter pylori is well known for its relationship with the occurrence of several severe gastric *** mechanisms of pathogenesis triggered by *** are less well *** this study, we report the genome seque...
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Background: Helicobacter pylori is well known for its relationship with the occurrence of several severe gastric *** mechanisms of pathogenesis triggered by *** are less well *** this study, we report the genome sequence and genomic characterizations of *** strain HLJ039 that was isolated from a patient with gastric cancer in the Chinese province of Heilongjiang, where there is a high incidence of gastric *** investigate potential genomic features that may be involved in pathogenesis of carcinoma, the genome was compared to three previously sequenced genomes in this area.
Background: Accumulating evidence demonstrates that complex diseases may arise from cooperative effects of multiple dysfunctional ***, identifying abnormal functions cooperatively regulated by multiple miRNAs is usefu...
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Background: Accumulating evidence demonstrates that complex diseases may arise from cooperative effects of multiple dysfunctional ***, identifying abnormal functions cooperatively regulated by multiple miRNAs is useful for understanding the pathogenesis of complex diseases.
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