Autophagy in atherosclerotic plaque macrophage contributes to the alleviation of atherosclerosis through the promotion of lipid metabolism.β-arrestins are multifunctional proteins participating various kinds of cellu...
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Autophagy in atherosclerotic plaque macrophage contributes to the alleviation of atherosclerosis through the promotion of lipid metabolism.β-arrestins are multifunctional proteins participating various kinds of cellular signaling *** we aimed to determine the role of β-arrestins in atherosclerosis and whether autophagy was involved in this ***-/-β-arrestin-1 fl/flLysM-Cre mice was created through bone marrow transplantation for the atherosclerosis model with conditional myeloid knocking out β-*** marrow-derived macrophages(BMDMs)were used for the in vitro *** red O staining was used to detect the lesional area.F4/80,Masson trichrome and picro-Sirius red staining were applied for the determination of plaque ***-time PCR was used for the detection of levels of lipid metabolism-related *** blotting,electron microscopy and tandem fluorescent mRFP-GFP-LC3 plasmid was applied to test autophagy *** found that β-arrestin-1,not β-arrestin-2,was highly increased in expression in plaque macrophage on the occurrence of *** myeloid knocking out β-arrestin-1 largely attenuated plaque formation and *** murine macrophage with lipid loading,knocking down β-arrestin-1 decreased foam cell formation and enhanced the level of cholesterol,while overexpressing β-arrestin-1 led to the reverse *** alleviative effects induced by macrophage β-arrestin-1 in atherosclerosis were involved in autophagy,based on the reduction of autophage level with the knocking down of macrophage β-arrestin-1 and the administration of autophagy inhibitors which largely attenuated the decreasing effect on foam cell *** results demonstrated for the first time that macrophage β-arrestin-1 protected against atherosclerosis through the induction of autophagy.
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