目的：建立苍耳果实中异槲皮苷、紫云英苷的HPLC方法,并测定和比较三种不同来源苍耳属植物果实中两种黄酮成分的含量,为苍耳属药用资源的开发与利用提供依据.方法：采用 Agilent Eclipse Plus C18(2.1mm×100mm,1.8μm)色谱柱,流动...
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目的：建立苍耳果实中异槲皮苷、紫云英苷的HPLC方法,并测定和比较三种不同来源苍耳属植物果实中两种黄酮成分的含量,为苍耳属药用资源的开发与利用提供依据.方法：采用 Agilent Eclipse Plus C18(2.1mm×100mm,1.8μm)色谱柱,流动相为0.4％磷酸水-乙腈,梯度洗脱,进样量为10μL,检测波长360nm,流速1mL·min-1;柱温35℃.结果：供试品在24小时内保持稳定,两种黄酮类成分在线性范围内相关系数r均大于0.9990,加样回收率(n=6)依次为100.40％和99.85％,其RSD值均≤3.00％.结论：所建立的方法准确、简单、重复性好,可用于苍耳果实中两种黄酮成分的含量测定;苍耳属植物意大利苍耳果实的异槲皮苷和紫云英苷含量最高,蒙古苍耳果实次之,苍耳果实中两种黄酮类成分含量较低.
An intensive karyotypeanalysis of Trichosanthes kirilowii Maxim.,a Chinese herb in the family Cucurbitaceae,was carried out using fluorescence in situ *** results showed that *** isoctoploid and has 88 chromosomes in ...
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An intensive karyotypeanalysis of Trichosanthes kirilowii Maxim.,a Chinese herb in the family Cucurbitaceae,was carried out using fluorescence in situ *** results showed that *** isoctoploid and has 88 chromosomes in somatic *** C-value of the female individuals is 5.12 pg/2C,while that of the male individuals is 4.83 pg/2C,with a difference of 0.1G between the males and *** results revealedthenumberof5SrDNAiseight,and the 45SrDNAsignal is ***,non-integral ploidy was not *** in situ hybridizationanalysisfound differences in the hybridization *** statistically analyzed the length and arm ratio of the chromosomes and obtained a preliminary formula for the female and male *** data implied that the karyotype of the *** isoctoploid,the genome of the female plant is larger than that of the male plant,and there is no sex chromosome,which makes *** a promising model for studying sex chromosome *** karyotype of plants in Trichosanthes were analyzed,and found that the plants were multiplied by 11 as the base number,which laid the foundation for the prediction of the genome size of other plants in the genus;then we analyzed the relationship between the karyotype and geographical distribution,and showed that the low ploidy plants were mainly distributed in the tropical and subtropical regions,while the high ploidy plants were mainly distributed in the temperate zone and the cold ***,according to the analysis of karyotype of Cucurbitaceae,We carried out a possible explanation for pollination phenomena of Trichosanthes.
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