A total of 276 endophytic bacteria isolated from root nodules of soybean (Glycine max L.) in the field of forthteen sites subordinated to nine districts of Henan province in China, were determined for inhibitory activ...
A total of 276 endophytic bacteria isolated from root nodules of soybean (Glycine max L.) in the field of forthteen sites subordinated to nine districts of Henan province in China, were determined for inhibitory activity against pathogenic fungus Fusarium graminearum 01 by initial and secondary screening in *** these strains, six strains with more than 63% inhibitory activity were characterized by optical epifluorescence microscopic observation, sequencing and phylogenetic analysis of 16S rRNA gene, potential plant growth-promoting traits analysis, plant inoculation assay and correspondence *** indicated that strain DD161 had the strongest inhibitory activity (71.14%) against the mycelial growth of *** 01, the mycelia of pathogenic fungus underwent morphological abnormal changes under the effect of endophytic bacteria, such as fracture, lysis, hyphae end became protoplast concentration and formed a ball, or became split ends.
Bacillus amyloliquefaciens FZB42 is a model organism for Gram positive soil bacteria associated with plant roots and beneficial to plant *** is of immense importance to understand metabolic mechanisms of this class of...
Bacillus amyloliquefaciens FZB42 is a model organism for Gram positive soil bacteria associated with plant roots and beneficial to plant *** is of immense importance to understand metabolic mechanisms of this class of bacteria adapting to *** this work employing differential RNA sequencing and Northern blot, we systematically identified mRNAs expressed in response to different growth phases as well as non-coding regulatory RNAs in FZB42.A large suit of TSSs and polycistronic operons were also identified and the genome annotation was *** result we obtained provides valuable data for investigation of Bacillus gene expression and molecular details of rhizobacterial interaction with host plants.
A novel bacterial strain designated YN-59T was isolated from chili rhizosphere soil in *** isolate was aerobic, Gram-positive, rod-shaped and formed ellipsoidal or oval spores positioned centrally in swollen *** the b...
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A novel bacterial strain designated YN-59T was isolated from chili rhizosphere soil in *** isolate was aerobic, Gram-positive, rod-shaped and formed ellipsoidal or oval spores positioned centrally in swollen *** the basis of 16S rRNA gene sequence analysis, the isolated strain YN-59 was related to members of genus *** levels of 16S rRNA gene sequence similarity were found between strain YN-59 and Cohnella plantaginis DSM 25424T (98.5%) and Cohnella ginsengisoli DSM18997T (97.3%), the 16S rRNA gene sequence similarities between strain YN-59 and the other strains recognized members of the genus Cohnella were below 97%.The DNA-DNA hybridization values of strain YN-59 with Cohnella plantaginis DSM 25424T and Cohnella ginsengisoli DSM18997T were 44.2±8.4 % and 28.8±5.8 %, *** DNA G+C content of strain YN-59r was 59.32mo1%.The major isoprenoid quinone was MK-7 and the predominant fatty acids were anteiso-C15∶0 (45.32%), iso-C16∶0 (19.19%), iso-C15∶0 (9.65%) and C16∶o (8.91%).The polar lipids of strain YN-59T consist of diphosphatidylglycerol, phosphatidylglycerol, lyso-phosphatidylglycerol, phosphatidylinositol, glycolipid, unknown phospholipid, phosphatidylcholine, aminolipid and phosphatidylinositol *** diamino acid in the cell wall was *** the basis of its phenotypic and genotypic characteristics and levels of DNA-DNA hybridization, strain YN-59T was considered to represent a novel species of the genus Cohnella, for which the name Cohnella chilisis ***.(Type strain YN-59T =CGMCC 1.12046T=JCM 19168T) is proposed.
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