Ginsenoside Rb1（GRb1） is one of main components from Panax ginseng,which was reported to enhance neurogenesis and inhibit neruoinflammatory *** ginseng has been widely prescripted to treat for affective disorders in...
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Ginsenoside Rb1（GRb1） is one of main components from Panax ginseng,which was reported to enhance neurogenesis and inhibit neruoinflammatory *** ginseng has been widely prescripted to treat for affective disorders in China and other *** study was designed to explore the mechanisms underlying the antidepressant-like effects of Ginsenoside Rb1 in mice exposed to chronic unpredictable mild stress（CUMS）,a model of major depressive disorder（MDD）.After 4 weeks of CUMS,the mice exhibited depression-like behaviors,and then the mice were treated with Ginsenoside Rb1（10mg/kg,intragastric administration） for another successive *** antidepressant effect of Ginsenoside Rb1 was evaluated by sucrose preference test,forced swimming test and tail suspension *** Quantitative real-time PCR was used to determine the level of pro-/anti-inflammatory cytokines in cortex and *** Immunohistochemistry was applied to detect the number of newborn neuron（Brdu/DCX） in *** was showed Ginsenoside Rb1 reversed anhedonia in sucrose preference test and decreased immobility time in the FST,TST in CUMS-treated C57BL/6J mice.A dysregulation between pro-inflammatory cytokines（IL-1β,TNF-α and IL-6） and anti-inflammatory cytokines（TGF-β,Arg1 and EL-10） was detected in mice model of *** Ginsenoside Rb1 administration,pro-inflammatory cytokines decreased and anti-inflammatory cytokines increased in cortex and hippocampus in MDD model *** numbers of newborn neuron（BrdU/DCX）in hippocampus were significantly increased in Ginsenoside Rb1-treated mice compared with saline *** data indicated that Ginsenoside Rb1 had antidepressant-like effects,due to it inhibiting inflammatory response and enhancing the hippocampal neurogenesis.
Previous studies have shown that Hedyotis Diffusa Willd(HDW),a traditional Chinese herbal medicine,exhibits potent anticancer activity in colorectal cancer *** forms of colorectal cancer can exhibit resistance to wide...
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Previous studies have shown that Hedyotis Diffusa Willd(HDW),a traditional Chinese herbal medicine,exhibits potent anticancer activity in colorectal cancer *** forms of colorectal cancer can exhibit resistance to widely used chemotherapeutic drugs,including the antimetabolite 5-fluorouracil(5-Fu),but less is known about the activity of HDW against 5-Fu resistant *** the present study,we investigate the mechanism of action and potency of ethanol extracts of HDW(EEHDW) on the multidrug-resistant colorectal cancer subline HCT-8/*** treatment significantly reduced cell viability of HCT-8/5-Fu cells in a dose and time-dependent manner as measured by the MTT cell proliferation ***,EEHDW significantly increased retention of the ATP-binding cassette(ABC) transporter substrate rhodamine-123(Rh-123) compared to untreated *** further investigate the molecular mechanisms targeted by EEHDW in resistant cells,we measured the expression of the ABC drug transporter protein P-gp and ABCG2 by RT-PCR and Western *** mRNA and protein expression levels of P-gp and ABCG2 were reduced in HCT-8/5-Fu cells following EEHDW *** data strongly suggest that EEHDW inhibits ABCG2-mediated drug resistance by down-regulating expression of ABCG2 and *** potential application of EEHDW as a chemotherapeutic adjuvant represents a promising alternative approach to the treatment of drug-resistant colorectal cancer.
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