目的:通过测定对照人群、颈动脉狭窄病人术前和动脉支架植入术后血浆中miRNA的表达水平,探讨miRNA在颈动脉狭窄病人及动脉支架植入术后血浆中的表达特征,寻找能指示颈动脉狭窄的miRNA。方法:收集2014年8月-2015年9月来我院就诊的颈动脉狭窄并进行动脉支架植入术病人28例和对照人群30例,用Real time PCR技术检测对照人群和颈动脉狭窄病人血浆中miR-146a、miR-4284、miR-4463、miR-4306、miR-210和miR-221的表达水平,并对颈内动脉支架植入术后24h、术后一个月和三个月复查病人进行随访检测。结果:与对照相比,miR-146a、miR-4284、miR-4463、miR-4306、miR-210、miR-221在颈动脉狭窄病人中表达分别升高5.48倍、13.83倍、23.37倍、21.02倍、3.87倍、3.05倍,P值均<0.01。颈内动脉支架植入术后24h时miR-146a、miR4284、miR-210和miR-221表达进一步升高,miR-4463和miR-4306表达下降至4.02倍和17.17倍,与对照相比差异具有统计学意义(P<0.05)。颈内动脉支架植入术后一月时miR-146a、miR-4284、miR-4463、miR-4306和miR-210表达水平分别降低至对照组的11.26倍(P<0.01)、6.17倍(P<0.01)、2.50倍(P<0.05)、13.39倍(P<0.01)和3.03倍(P<0.01),miR-221表达水平与对照无显著差异(P>0.05),颈内动脉支架植入术后三月时miR-146a、miR-4284、miR-4463、miR-4306和-miR-210表达水平重新回升至对照组的12.85倍、27.00倍、108.47倍、35.20倍和24.48倍,差异均有统计学意义,而miR-221表达水平与对照无差异(P>0.05)。结论:miR-146a、miR.4284、miR-4463、miR4306、miR-210和miR-221的表达水平与颈动脉狭窄相关,其表达水平的上升可能提示颈动脉狭窄的发生。与术前相比,miR-146a、miR-4284、miR-4463、miR4306和miR-21O表达水平在术后一月时出现降低但在术后三个月时重新升高,这些miRNA与颈动脉狭窄程度的相关性需更长时间的跟踪检测。与术前相比,术后一月和三月时miR-221表达水平显著降低,提示该miRNA可能是一个潜在的颈动脉狭窄标志分子。
Objectives:Refractory wounds in diabetic patients constitute a serious complication that often leads to amputation with limited treatment *** stress has been proposed as an important pathogenic factor in diabetic woun...
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Objectives:Refractory wounds in diabetic patients constitute a serious complication that often leads to amputation with limited treatment *** stress has been proposed as an important pathogenic factor in diabetic wound *** present study was designed to determine the protective effect of the GTP cyclohydrolaseⅠ(GTPCH),the rate-limiting enzyme of BH4 synthesis;soy isoflavone genistein and Ganoderma lucidum polysaccharide(Gl-PS)on diabetic wound healing.
Objective:Platelets can regulate endothelial cell genes expression through their adhesion to the subendothelium in response to vascular *** it has been reported that some endothelial cells(ECs)-secreted genes can modu...
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Objective:Platelets can regulate endothelial cell genes expression through their adhesion to the subendothelium in response to vascular *** it has been reported that some endothelial cells(ECs)-secreted genes can modulate vascular smooth muscle cells(VSMCs) phenotypic transformation by ECs/VSMCs ***,little is known about the effects of platelets adhesion to ECs interaction on
Objective:The present study investigated the effects of Hydroxysafflor yellow A(HSYA)on angiogenesis in vitro and in a mouse hindlimb ischemia ***:Human umbilical vascular endothelial cells(HUVECs)were treated with HS...
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Objective:The present study investigated the effects of Hydroxysafflor yellow A(HSYA)on angiogenesis in vitro and in a mouse hindlimb ischemia ***:Human umbilical vascular endothelial cells(HUVECs)were treated with HSYA and then cell capillary-like tube formation,migration and the levels of angiopoietin 1(Ang 1)and Tie-2 protein expression were *** anti-Tie-2neutralizing antibody was used to investigate the mechanism of HSYA-induced
Purpose:Mitochondrial dysfunction is a prominent feature of ischemia heart disease but the underlying mechanism of dynamics(fusion/fission)is still *** we investigated a novel function and underlying mechanism of Rg1 ...
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Purpose:Mitochondrial dysfunction is a prominent feature of ischemia heart disease but the underlying mechanism of dynamics(fusion/fission)is still *** we investigated a novel function and underlying mechanism of Rg1 on an in vitro cardiomyocyte model ofhypoxia/reoxygenation(H/R).Methods:Cellular cytotoxicity was evaluated by MTT,mitochondrial viable staining,and cardiac markers *** function was evaluated by ATP content
Diabetes mellitus(DM) leads to the development of microvascular diseases and is associated with impaired *** presence of VEGF can block PDGF-BB dependent regulation of neovascularization and vessel *** tested the hypo...
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Diabetes mellitus(DM) leads to the development of microvascular diseases and is associated with impaired *** presence of VEGF can block PDGF-BB dependent regulation of neovascularization and vessel *** tested the hypothesis that the inhibition of VEGF improves blood flow in a mouse hindlimb ischemia *** this study,we examined the effect of bevacizumab,a humanized monoclonal antibody against VEGF-A,on blood perfusion after hindlimb ***,we showed that bevacizumab induces functional blood flow in high fat chow(HFC)-fed diabetic *** with bevacizumab increased the expression of PDGF-BB in ischemic muscle,and led to vascular *** also blocked vascular leakage by improving the recruitment of pericytes associated with nascent blood vessels,but it did not affect capillary ***,treatment with an anti-PDGF drug significantly inhibited blood flow perfusion in diabetic mice treated with *** results indicate that bevacizumab improves blood flow recovery through the induction of PDGF-BB in a diabetic mouse hindlimb ischemia model,and that vessel normalization may represent a useful strategy for the prevention and treatment of diabetic peripheral arterial disease.
Aim:Arteriosclerosis obliterans(ASO) of the lower extremities is a major cause of adult limb loss worldwide.A timely diagnosis in the early stages of the disease determines the clinical outcomes,however lacking of pal...
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Aim:Arteriosclerosis obliterans(ASO) of the lower extremities is a major cause of adult limb loss worldwide.A timely diagnosis in the early stages of the disease determines the clinical outcomes,however lacking of palpable symptoms remains the biggest *** study aimed to screen a cluster of microRNAs(miRNAs) that can be used as biomarker for the ASO in the earlier ***:Plasma from 3 patients with ASO and 3 healthy controls were profiled to screen altered miRNAs by microarray,then Real time PCR was further used to confirm the changes in 55 ASO patients and 54 *** also analyzed the correlation of miRNAs level with Fontaine stages and the influence of T2 DM which is a common complication with ASO on the level of ***:Twenty-four aberrantly expressed miRNAs were screened in the plasma of ASO *** time PCR verified that the level of miR-4284 was significantly increased,while levels of miR-4463,miR-4306 and miR-221-3p were significantly decreased both in the plasma and in the sclerotic samples compared with the ***,we revealed a time and stage specific expression manner,as shown that expression of miR-4284 increased at the stage I of ASO and maintained the tendency to stage Ⅳ,while miR-4463 expression decreased at every stage of ASO;however,the expression of miR-4463 showed opposite changes in ASO patients with or without T2 ***:Altered expressions of miR-4284 and miR-4463 are novel characteristics and may serve as potential biomarkers for the early diagnosis of ASO.
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