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检索条件"任意字段=第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛"

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Panax Notoginseng Saponins Decrease Cholesterol Ester via Up-regulating ATP-Binding Cassette Transporter A1 in Foam Cells

Panax Notoginseng Saponins Decrease Cholesterol Ester via Up...

引用

第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者： Yi Jia~a,Zhuo-Ying Li~a,Hai-Gang Zhang~a,Ya Liu~a,and Xiao-Hui Li~(a*) (a Institute of Materia Medica and Department of Pharmaceutics,College of Pharmacy,Third Military Medical University,30 Gaotanyan,Shapingba,Chongqing,400038,People's Republic of China)

Panax notoginseng Saponins(PNS) are the effective ingredients extracted from Panax Notoginseng (Burk.) ***,which has been used to prevent cardiovascular diseases and enhance wound healing for more than one thousand years in *** of the active constituents are derivatives of 20(S) -protopanaxadiol,20(S) -protopanaxpanaxatriol or dammarane-type *** addition to the aforementioned results,in vitro study is also one requisite for the complete elucidation of PNS pharmacological *** determine the potential cytotoxicity of PNS,a cell viability test via MTT method was performed first to examine the possible membrane integrity change caused by saponins due to their physicochemical *** result revealed that the cell viability was not affected by treatments with ox-LDL or ***,it is clear that(in) our test,cells utilized stored lipids when macrophages<正>Panax notoginseng Saponins(PNS) are the effective ingredients extracted from Panax Notoginseng (Burk.) ***,which has been used to prevent cardiovascular diseases and enhance wound healing for more than one thousand years in *** of the active constituents are derivatives of 20(S) -protopanaxadiol,20(S) -protopanaxpanaxatriol or dammarane-type *** addition to the aforementioned results,in vitro study is also one requisite for the complete elucidation of PNS pharmacological *** determine the potential cytotoxicity of PNS,a cell viability test via MTT method was performed first to examine the possible membrane integrity change caused by saponins due to their physicochemical *** result revealed that the cell viability was not affected by treatments with ox-LDL or ***,it is clear that(in) our test,cells utilized stored lipids when macrophages

关键词： Panax Notoginseng Saponins Decrease Cholesterol Ester via Up-regulating ATP-Binding Cassette Transporter A1 in Foam Cells ATP

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牛磺酸抑制心肌成纤维增殖的信号转导机制研究

牛磺酸抑制心肌成纤维增殖的信号转导机制研究

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第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者：王立英李红张问杨世杰吉林大学白求恩医学院药理教研室

目的研究牛磺酸（taurine,Tau）对血管紧张素Ⅱ（angiotensin,AngⅡ）诱导的新生大鼠心肌成纤维细胞（cardiacfibroblasts,CFbs）增殖抑制作用的机制研究。方法胰酶消化法分离培养新生大鼠心肌成纤维细胞,用AngⅡ诱导CFbs增殖,建立心肌... 详细信息

目的研究牛磺酸（taurine,Tau）对血管紧张素Ⅱ（angiotensin,AngⅡ）诱导的新生大鼠心肌成纤维细胞（cardiacfibroblasts,CFbs）增殖抑制作用的机制研究。方法胰酶消化法分离培养新生大鼠心肌成纤维细胞,用AngⅡ诱导CFbs增殖,建立心肌纤维化模型。实验采用2-3代的CFbs,分为正常对照组、AngⅡ组、Tau治疗组、D4681（PKCα特异性抑制剂L-苏式神经酰胺）组及AG（iNOS特异抑制剂氨基胍）组。1)采用四甲基偶氮唑蓝（MTT）法检测细胞增殖;羟脯氨酸试剂盒检测CFbs总的胶原含量;ELISA法测定Ⅰ型胶原、Ⅲ型胶原的含量;硝酸还原酶法检测细胞上清液一氧化氮（nitric oxide,NO）的含量;采用诱导型一氧化氮合酶（inducible nitric oxide synthase,iNOS）试剂盒测定iNOS活性。2)免疫细胞化学法及荧光共聚焦测定p-PKCα的膜转位和表达、iNOS的表达及p-ERK1/2的核转位和表达;免疫荧光及免疫化学染色法检测NF-κB p65的核转位和蛋白表达。3)Western blot法检测p-PKCα、iNOS和p-ERK1/2蛋白表达和含量。结果 1)牛磺酸（30、60和120）mmol·L可剂量依赖性的抑制AngⅡ诱导的CFbs增殖;减少Ⅰ型胶原、Ⅲ型胶原合成;提高CFbs的NO含量和增加iNOS活性,与D4681具有协同作用;应用AG后,NO含量和iNOS活性均降低,但细胞增殖率升高。与AngⅡ组相比差异显著（P<0.01）。2)免疫细胞化学法及荧光共聚焦可见Tau（60和120）mmol·L能明显抑制p-PKCα的膜转位和表达、p-ERK1/2的核转位和表达,D4681能加强Tau的抑制作用,但AG没有此作用,与AngⅡ组相比差异显著（P<0.05）;同时Tau能促进iNOS在胞浆中的表达,D4681与Tau具有协同效应,而AG能抑制iNOS的表达,与AngⅡ组比较差异显著（P<0.01）;免疫荧光及免疫化学染色法检测可见Tau（60和120）mmol·L能抑制NF-κB p65的核转位和蛋白表达,与AngⅡ组比较差异显著（P<0.01）。3)牛磺酸（60和120）mmol·L可明显抑制p-PKCα、iNOS和p-ERK1/2的蛋白表达,D4681能加强Tau抑制p-PKC和p-ERK1/2的蛋白表达,AG没有此作用;D4681能加强Tau促进iNOS蛋白表达,而AG却减少iNOS蛋白的生成。结论牛磺酸通过抑制p-PKCα的膜转位和表达,抑制p-ERK1/2的核转位和表达,抑制NF-κB p65的核转位,提高CFbs的iNOS活性,增加NO的含量,即通过PKC-MAPK、PKCα-iNOS信号转导通路调控CFbs的增殖;从而抑制CFbs胶原含量的增加,最终抑制心肌纤维化。

关键词：牛磺酸心肌成纤维细胞血管紧张素Ⅱ 蛋白激酶Cα 一氧化氮诱导型一氧化氮合酶 NF-κB p65 细胞外信号调节激酶1/2

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Ang-1、Ang-2通过Tie-2受体调节大鼠失血性休克血管反应性双相变化

Ang-1、Ang-2通过Tie-2受体调节大鼠失血性休克血管反应性双相变化

引用

第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者：徐竞李涛杨光明刘良明

目的本实验室前期研究发现,失血性休克后血管反应性呈早期增高和晚期降低的双相变化,血管生成素-1(angiopoietin-1,Ang-1)、血管生成素-2(angiopoietin-2,Ang-2)在失血性休克后的肠系膜上动脉(superior mesenteric artery,SMA)中呈时间... 详细信息

目的本实验室前期研究发现,失血性休克后血管反应性呈早期增高和晚期降低的双相变化,血管生成素-1(angiopoietin-1,Ang-1)、血管生成素-2(angiopoietin-2,Ang-2)在失血性休克后的肠系膜上动脉(superior mesenteric artery,SMA)中呈时间上的差异表达(Ang-1的蛋白表达早期增高、晚期降低,Ang-2的蛋白表达在休克早期变化不大,在休克后期逐渐增高),且这种差异表达参与了失血性休克血管反应性双相变化的形成,但具体机制如何尚不清楚。根据基础研究,Ang-1和Ang-2属于血管内皮细胞(vascular endothelia cell,VEC)特异的促血管新生因子家族——血管生成素家族,主要的受体是VEC中的Tie-2,但Ang-1和Ang-2对Tie-2的激动作用相反,从而共同调节血管形成。那么,在失血性休克后的肠系膜上动脉中,呈时间上差异表达的Ang-1和Ang-2是否通过对Tie-2的不同作用,从而导致了休克血管反应性双相变化的形成?本实验探讨Tie-2在Ang-1和Ang-2调节失血性休克血管反应性双相变化中的作用。方法采用离体微血管环张力测定技术和western blot技术,观察失血性休克后不同时间点SMA中Tie-2蛋白表达和磷酸化变化、Tie-2封闭抗体对Ang-1和Ang-2调节缺氧早期和晚期血管反应性作用的影响,以及给予Ang-1和Ang-2后缺氧VEC中Tie-2蛋白表达和磷酸化变化。结果 (1)肠系膜上动脉Tie-2蛋白表达和酪氨酸磷酸化在正常时很低,失血性休克后逐渐增高,在休克早期(休克1 0min),Tie-2蛋白表达变化不大,但酪氨酸磷酸化已明显增高(P<0.01);随着休克时间延长,Tie-2蛋白表达和酪氨酸磷酸化均进一步显著增高(P<0.01)。(2)Tie-2抑制剂可降低缺氧10min的血管高反应性(NE的Emax由13.479mN降低至10.122mN,P<0.05),并显著抑制Ang1对缺氧10min血管反应性的维持作用(Emax由15.283mN降低至10.253mN,P<0.01);Tie-2抑制剂可改善缺氧4h的血管低反应性(NE的Emax由5.875mN增高至8.003mN,P<0.05),并显著拮抗Ang2进一步降低缺氧4h血管反应性的作用(Emax由3.444mN增高至7.643mN,P<0.01)。(3)缺氧10min时,降低血管高反应性的Ang2可降低Tie-2磷酸化,使其由0.0403降低至0.0123(P<0.01);缺氧4h时,恢复血管低反应性的Ang1可降低Tie-2蛋白表达,使其由0.2276降低至0.0851(P<0.01),也可以降低Tie-2磷酸化,使其由0.1437降低至0.0359(P<0.01)。结论 Ang-1、Ang-2通过Tie-2受体调节大鼠失血性休克血管反应性双相变化。

关键词： Ang-2 Tie-2 失血性休克受体调节

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蒺藜皂苷的化学物质基础及单体B对心肌细胞缺血再灌注损伤的保护作用研究

蒺藜皂苷的化学物质基础及单体B对心肌细胞缺血再灌注损伤的保护...

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第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者：张爽李红焦燕杨世杰吉林大学白求恩医学院药理学系

目的分离蒺藜皂苷单体,筛选具有心肌细胞保护作用的单体,确定蒺藜皂苷的化学物质基础,并检测其对心肌缺血再灌注损伤的保护作用及初步探讨其作用机制。方法分离乳鼠心肌细胞,采用HO制备心肌细胞损伤模型,筛选单体A、B、C、D、E、F、G、J... 详细信息

目的分离蒺藜皂苷单体,筛选具有心肌细胞保护作用的单体,确定蒺藜皂苷的化学物质基础,并检测其对心肌缺血再灌注损伤的保护作用及初步探讨其作用机制。方法分离乳鼠心肌细胞,采用HO制备心肌细胞损伤模型,筛选单体A、B、C、D、E、F、G、J、I中有保护作用的单体。心肌细胞随机分为,正常对照组（Control）、缺氧复氧模型组（H/R）、蒺藜皂苷组（GSTT）、蒺藜皂苷单体B（TTSMB）10、1、0.1 nmol·L组,PKC抑制剂及ERK1/2抑制剂PD98059组,缺氧3h,再给氧2h,观察细胞形态学变化,应用MTT比色法检测细胞存活率,测定培养基中肌酸激酶（CK）、乳酸脱氢酶（LDH）、天门冬氨酸氨基转移酶（AST）释放量,超氧化物歧化酶（SOD）活性,丙二醛（MDA）含量,并采用流式细胞仪检测心肌细胞凋亡率及应用western blot检测TTSMB对凋亡相关蛋白caspase-3的作用。应用PKCε及ERK1/2抑制剂,采用免疫组织化学、激光共聚焦及western blot检测PKCε及ERK1/2表达。建立离心工作心模型,大鼠随机分为正常对照组,缺血再灌注损伤组,蒺藜皂苷组（GSTT）、蒺藜皂苷单体B（TTSMB）100、10、1 nmol·L组,PKC抑制剂Cherrythine组,缺血30min,再灌注120min,检测灌流液中心肌酶及心肌组织中SOD活性及MDA含量,HE染色及TUNEL法观察心肌组织形态变化;western blot检测凋亡相关蛋白bcl-2,bax、caspase-3及PKCε表达,免疫组织化学检测p-PKCε表达。结果 HO模型粗筛显示,单体B与G对氧化应激损伤的心肌细胞具有保护作用。细胞实验结果显示,与模型组比较,TTSMB 10、1、0.1 nmol·L组存活心肌细胞数明显增多（P<0.05,P<0.01）,心肌细胞培养液中CK、LDH、AST含量降低,SOD活力增加、MDA含量降低（P<0.05,P<0.01）,心肌细胞凋亡率明显下降,caspase-3表达亦下降,心肌细胞PKCε及ERK1/2表达明显增加,而应用PKC及ERK1/2抑制剂PD98059后,PKCε及ERK1/2表达明显减少。离体心结果显示,应用TTSMB后,心肌酶漏出减少,心肌细胞氧化损伤明显减轻,凋亡相关蛋白bcl-2表达增多,bax及caspase-3表达下降,PKCε表达明显增多。结论 TTSMB可减轻心肌细胞缺血再灌注损伤,抑制心肌细胞调亡,具有心肌细胞保护作用,其机制与激活PKCε及ERK1/2通路有关。

关键词：蒺藜皂苷单体心肌细胞缺血再灌注损伤(I/R) 凋亡 PKCε ERK1/2

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High Density Lipoprotein Promotes Glucose Utilization and Ameliorates Metabolic Phenotypes of Diabetic db/db Mice

High Density Lipoprotein Promotes Glucose Utilization and Am...

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第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者：张启春郭瑞万蓉张贇李胜男南京医科大学药理学系

OBJECTIVE—Lower quantity and quality of high density lipoprotein(HDL) are important characteristics of type 2 diabetes *** HDL infusion results in obvious fall of plasma glucose in *** study aims to investigate the underlying mechanisms mediating HDL actions on glucose metabolism,and the therapeutic effects of long-term HDL infusion on diabetic db/db mice. REASEARCH DESIGN AND METHODS—Actions of HDL on glucose uptake and glucose transporter 4(GLUT4) translocation were assessed with l-[H]-2-deoxyglucose and plasma membrane lawn,respectively,in 3T3-L1 *** analysis was performed with amyloglucosidase both in vitro and in *** phenotypes of db/db mice with long-term HDL infusion were monitored by analyzing metabolism parameters,adipose tissue function,inflammatory cytokines and oxidative-stress indexes. Corresponding signaling molecules were detected by western blot. RESULTS—HDL had a great influence on glucose *** stimulated glucose uptake in 3T3-L1 adipocytes in a time- and concentration-dependent manner with maximal effects at about 20 min (50μg protein/ml),and apoA-I is one of the main components to mediate this action through PI3K/Akt and eNOS associated ***4 translocation was also significantly increased,the net amount of GLUT4 in plasma membrane were significantly resulted from increase in exocytosis through AKT and AMPKa pathway,and also decrease in endocytosis mediated by *** deposition got enhanced by HDL in L6 cells and gastrocnemius muscle,paralleling with elevated GSK3 phosphorylation. Meanwhile,increased Akt-Ser473 and AMPKa phosphorylation were detected in HDL-treated adipocytes and gastrocnemius ***,HDL was found to change the phenotypes of db/db mice. Long-term HDL infusion could pronouncedly reduced blood glucose,and improve insulin sensitivity and inhibit pancreatic islets proliferation with interesting insulin secretion enhancement in rodent models with obesity a

关键词： High Density Lipoprotein Promotes Glucose Utilization and Ameliorates Metabolic Phenotypes of Diabetic db/db Mice

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人工麝香对大鼠高脂血症影响的实验研究

人工麝香对大鼠高脂血症影响的实验研究

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第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者：李海涛朱雪晶吴其标南京中医药大学药学院澳门科技大学中医药学院

麝香具有开窍醒神、活血通经、消肿止痛、解毒功效,常用于热病神昏、跌仆伤痛等,是名贵中药之一。目的:研究发现,人工麝香具有耐缺氧、抑制心肌酶释放、减少心肌缺血梗死面积等发挥抗心肌缺血作用。但人工麝香对高脂血症的影响未见报道... 详细信息

麝香具有开窍醒神、活血通经、消肿止痛、解毒功效,常用于热病神昏、跌仆伤痛等,是名贵中药之一。目的:研究发现,人工麝香具有耐缺氧、抑制心肌酶释放、减少心肌缺血梗死面积等发挥抗心肌缺血作用。但人工麝香对高脂血症的影响未见报道。为进一步开发其药用价值,本研究旨观察人工麝香对大鼠高脂血症的影响。方法:SD大鼠,随机分为6组,每组10只。空白组、模型组、阳性组（辛伐他汀2 mg/kg）、人工麝香低剂量组（3 mg/kg）、人工麝香中剂量组（6 mg/kg）、人工麝香高剂量组（12 mg/kg）。分组后,空白组正常饲养。其余每组维生素D70万IU/kg的总剂量灌胃,分4次,隔2d注射一次。之后,每组大鼠1 mL/100g灌胃高脂乳剂,每日1次,连续4周。阳性组同时给药辛伐他汀2 mg/kg,人工麝香低、中、高剂量组分别灌胃给予3、6、12 mg/kg,每日1次,连续4周。末次给药后1 h,颈总动脉取血,3500 r/min离心10 min,分离血清备用。完整取出肝脏,观察表面颜色并称重,计算肝/体比值。检测TC、TG、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）含量,用双抗体夹心ELISA法测定血清CRP、内皮素-1（ET-1）、TNF-α的变化。结果:1)各组大鼠体重与肝重指数的变化实验前后,大鼠体重有明显变化:与空白组比较,模型组、辛伐他汀组、人工麝香3、6、12 mg/kg组体重增重率[（实验后体重—实验前体重）/实验前体重]明显降低,差异有统计学意义（P<0.01）;与模型组比较,空白组、辛伐他汀组、人工麝香3、6、12 mg/kg组体重增重率明显升高,差异有统计学意义（P<0.05）。肝重指数变化:与空白组比较,模型组、辛伐他汀组、人工麝香3、6、12 mg/kg组肝重指数变化率明显升高,差异有统计学意义（P<0.05）;与模型组比较,空白组肝重指数变化率降低,差异有统计学意义（P<0.05）;2)人工麝香对大鼠血清TC、TG、HDL-C、LDL-C的影响与空白组比较,模型组TC、TG升高显著,有统计学差异（P<0.01）;HDL-C、LDL-C升高明显,差异有统计学意义（P<0.05）。与模型组比较,辛伐他汀组TC、TG、LDL-C明显降低（P<0.05）,人工麝香3、6、12 mg/kg TG明显降低（P<0.05）,人工麝香12 mg/kg组TC明显降低,差异有统计学意义（P<0.05）;3)人工麝香对大鼠血清CRP、ET-1、TNF-α的影响与空白组比较,模型组CRP、TNF-α明显升高,有统计学差异（P<0.05）,ET-1显著升高（P<0.01）。与模型组比较,辛伐他汀组CRP、ET-1、TNF-α明显降低（P<0.05）,人工麝香12 mg/kg CRP、ET-1明显降低,差异有统计学意义（P<0.05）。结论:人工麝香对大鼠高脂血症具有明显的调节作用。

关键词：人工麝香大鼠高脂血症模型组

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Effect of integrinβ1 on uptaking ox-LDL in RAW264.7 cells

Effect of integrinβ1 on uptaking ox-LDL in RAW264.7 cells

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第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者： Jian Tao,Xiao-hui Li,Ya Liu (Institute of Materia Medica,college of pharmacy,Third Military Medical University,Chongqing 400038, China) 第三军医大学药学院药物研究所

Objective To discuss the effect of integrinβ gene on uptaking ox-LDL in RAW264.7 *** Three siRNAs were designed according to different targets on integrinPi gene were transfected into RAW264.7 cells by lipidosom *** RAW264.7 cells were divided into control （without transfection）,siRNANC（with transfection of siRNAnegative）,lipo2000（without transfection but added lipo2000）,siRNA（with transfection of effective siRNA）.SiRNA marked by Cy3 was used for transfection efficiency detection,and real-time PCR was used for siRNA with highest silence efficiency *** change of Cell adhesion was analyzed by cell adhesion experiment,and that of cell uptaking ox-LDL function was evaluated by the formation of lipid granules in the cells with oil red staining. Results We succeed to transfect siRNA into RAW264.7 cells as well as screen highest silence efficiency （65%） *** expression of integrinβ mRNA was obviously decreased in siRNA 1 group,in which cell adhesion was decrease form 70%to 53%as well as the foam cell formation decreased from 90%to 17%.Statistics analysis shows the siRNA 1 group has significant difference compared with oher groups（P < 0.05）.Conclusion The integrinβ gene affect the function of uptaking ox-LDL in RAW264.7 and then inhibit its transformation to foam cell.<正>Objective To discuss the effect of integrinβ gene on uptaking ox-LDL in RAW264.7 *** Three siRNAs were designed according to different targets on integrinPi gene were transfected into RAW264.7 cells by lipidosom *** RAW264.7 cells were divided into control （without transfection）,siRNANC（with transfection of siRNAnegative）,lipo2000（without transfection but added lipo2000）,siRNA（with transfection of effective siRNA）.SiRNA marked by Cy3 was used for transfection efficiency detection,and real-time PCR was used for siRNA with highest silence efficiency *** change of Cell adhesion was analyzed by cell adhesion experiment,and that of cell uptaking ox-LDL f

关键词： integrinβ1 foam cell ox-LDL

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Prenatal Exposure to Inflammation Induced by Zymosan Results in Activation of Intrarenal Renin-Angiotensin System in Adult Offspring Rats

Prenatal Exposure to Inflammation Induced by Zymosan Results...

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第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者： Xue-Qin Hao,Hai-Gang Zhang,Shu-Hui Li,Yi Jia,Ya Liu,Jian-Zhi Zhou, Yan-Ling Wei,Li-Yun Hao,Yuan Tang,Min Su,and Xiao-Hui Li (Institute of Materia Medica,College of Pharmacy,Third Military Medical University,Chongqing 400038,China)

Prenatal exposure to inflammation produces offspring that are hypertensive in *** present study was to explore the role of intrarenal renin-angiotensin(Ang) system in the development of hypertension programmed by prenatal exposure to *** rats were randomly divided into control group and zymosan group(n=6).Rats in these two groups were administered intraperitoneally with 0.5 ml vehicle and 2.37 mg/kg zymosan,respectively,on the eighth,tenth,and 12th day during gestation. The results showed the glomerular number and creatinine clearance rate decreased significantly in offspring of zymosan-treated *** renal cortex renin mRNA expression,AngⅡ-positive cells in renal cortex,and AngⅡexpression in renal medulla increased significantly in offspring of zymosan-treated rats at 7,16,and 25 weeks of *** plasma renin activity and AngⅡconcentration were *** conclusion, prenatal exposure to zymosan resulted in the activation of intrarenal renin-Ang system in adult offspring rats.<正>Prenatal exposure to inflammation produces offspring that are hypertensive in *** present study was to explore the role of intrarenal renin-angiotensin(Ang) system in the development of hypertension programmed by prenatal exposure to *** rats were randomly divided into control group and zymosan group(n=6).Rats in these two groups were administered intraperitoneally with 0.5 ml vehicle and 2.37 mg/kg zymosan,respectively,on the eighth,tenth,and 12th day during gestation. The results showed the glomerular number and creatinine clearance rate decreased significantly in offspring of zymosan-treated *** renal cortex renin mRNA expression,AngⅡ-positive cells in renal cortex,and AngⅡexpression in renal medulla increased significantly in offspring of zymosan-treated rats at 7,16,and 25 weeks of *** plasma renin activity and AngⅡconcentration were *** conclusion, prenatal exposure to zymosan resulted in the activation of intrarenal

关键词： Prenatal Exposure to Inflammation Induced by Zymosan Results in Activation of Intrarenal Renin-Angiotensin System in Adult Offspring Rats

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Persistent Systemic Inflammation Accelerates Atherosclerosis Lesion and Effects of Saponins of Panax Notoginseng in Rabbits

Persistent Systemic Inflammation Accelerates Atherosclerosis...

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第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者： Yi JIA,Ya LIU,Hai-gang ZHANG,Xiao-hui LI (Institute of Materia Medica,college of pharmacy,Third Military Medical University,Chongqing 400038,PR China)

Objective:To explore the roles of persistent systemic inflammation on atherosclerosis and the effects of saponins of Panax Notoginseng(PNS) in ***:Thirty rabbits were divided randomly into six groups,***,hyper-fat diet,inflammation,aspirin,PNS and simple-inflammation *** the animals except that in control group and simple-inflammation group were fed with hyper-fat diet for 8 *** on that,rabbits in inflammation,aspirin and PNS groups were treated with zymosan injection (10mg/kg,ip).Normal saline was given to rabbits as *** zymosan injection,animals in aspirin and PNS group were administrated with aspirin(12 mg/kg,ig) and PNS(120 mg/kg,ig).The animals in simple-inflammation group were treated with zymosan injection(10mg/kg,ip) and fed with normal diet. The atherosclerosis lesion in aortas was stained by Sudan *** total cholesterol,triacylglycerol, TNF-αand activity of post-heparin lipoprotein lipase were measured at the end of the 4th and 8th week, after an overnight ***:Compared with hyper-fat diet group,the area of atherosclerosis lesion, serum TG and TNF-αwere markedly increased in rabbits of inflammation group,and the activity of LPL was decreased *** TNF-αlevel was negatively correlated with the activity of post-heparin LPL(r=-0.708,p<0.01).The area of atherosclerosis,serum TG and TNF-αwere decreased in aspirin and<正>Objective:To explore the roles of persistent systemic inflammation on atherosclerosis and the effects of saponins of Panax Notoginseng(PNS) in ***:Thirty rabbits were divided randomly into six groups,***,hyper-fat diet,inflammation,aspirin,PNS and simple-inflammation *** the animals except that in control group and simple-inflammation group were fed with hyper-fat diet for 8 *** on that,rabbits in inflammation,aspirin and PNS groups were treated with zymosan injection (10mg/kg,ip).Normal saline was given to rabbits as *** zymosan inj

关键词： Persistent Systemic Inflammation Accelerates Atherosclerosis Lesion and Effects of Saponins of Panax Notoginseng in Rabbits

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Improved liquid chromatography-tandem mass spectrometry method for analysis of eptifibatide in human plasma

Improved liquid chromatography-tandem mass spectrometry meth...

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第十届全国心血管药理学术会议暨2010（重庆）国际心血管疾病与药物高峰论坛

作者： Zhi-Ling ZHOU,Xi-Yong YU~*,Min YANG,Li-Ping MAI,Qiu-Xiong LIN,Chun-Yu DENG,Ping ZHU, Tie-Feng CHEN,Xiao-Zhong HUANG,Zhi-Xin SHAN,Xiao-Ying LIU,Shu-Guang LIN Clinical Pharmacology Institute of Guangdong Academy of Medical Sciences Medical Research Center of Guangdong General Hospital Guangzhou 510080,PR China

Background:Eptifibatide binds to the platelet receptor glycoprotein(GP)Ⅱb/Ⅲa of human platelets and inhibits platelet *** the treatment of patients with acute coronary syndrome, including patients who are to be managed medically and those undergoing percutaneous coronary intervention(PCI),eptifibatide has been shown to decrease the rate of a combined endpoint of death or new myocardial infarction。Objective:An inproved,rapid and sensitive high performance liquid chromatography-tandem mass spectrometry method(LC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of eptifibatide in human ***:Eptifibatide and the internal standard(IS ),EPM-05,were extracted from plasma samples using solid phase extraction with Oasis HLB *** separation was performed on a Ultimat XB-C18 column (125mm×4mm i.d.,5μm particle size) using acetonitrile-water(10mmol/L ammonium acetate buffer,<正>Background:Eptifibatide binds to the platelet receptor glycoprotein(GP)Ⅱb/Ⅲa of human platelets and inhibits platelet *** the treatment of patients with acute coronary syndrome, including patients who are to be managed medically and those undergoing percutaneous coronary intervention(PCI),eptifibatide has been shown to decrease the rate of a combined endpoint of death or new myocardial infarction。Objective:An inproved,rapid and sensitive high performance liquid chromatography-tandem mass spectrometry method(LC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of eptifibatide in human ***:Eptifibatide and the internal standard(IS ),EPM-05,were extracted from plasma samples using solid phase extraction with Oasis HLB *** separation was performed on a Ultimat XB-C18 column (125mm×4mm i.d.,5μm particle size) using acetonitrile-water(10mmol/L ammonium acetate buffer,

关键词： EPM PCI Improved liquid chromatography-tandem mass spectrometry method for analysis of eptifibatide in human plasma

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