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Characterization of phenol-degrading bacterial strains isolated from natural soil

Characterization of phenol-degrading bacterial strains isola...

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第十次全国环境微生物学术研讨会

作者： Xiaojun Dong Qing Hong Lijuan He Xin Jiang Shunpeng Li Key Lab of Microbiological Engineering Agricultural Environment,Ministry of Agriculture,College of Life Science,Nanjing Agricultural University,Nanjing 210095 State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Science,Nanjing,210008

Six phenol-degrading bacteria designated as PND-1～PND-6 were isolated from natural ***-1～PND-6 were from the genera of Pseudomonas,Acinetobacter,Comamonas and Cupriavidus,*** these strains were able to utilize phenol as the sole carbon source to support *** of them could tolerate phenol with concentration of 6mM or more,but strain PND-3 could only tolerate lmM *** sequences of the partial largest subunit of multicomponent phenol hydroxylase (LmPH) gene were compared among these *** was found that the physiological groupings in the phylogenetic tree formed by their 16S rDNA sequences was correlated with that based on their partial amino acid sequences of LmPH,which indicated the potential application of LmPH as a molecular marker for the phylogenetic analysis of phenol-degrading *** of the six strains degraded phenol through catechol ortho fission pathway,whereas strain PND-3 and PND-6 harbored both ortho and meta fission pathways simultaneously.

关键词： Phenol Degradation Diversity LmPH Molecular marker Metabolism pathway

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不同利用方式对红壤甲烷营养菌丰度的影响

不同利用方式对红壤甲烷营养菌丰度的影响

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第十次全国环境微生物学术研讨会

作者：郑勇贺纪正中国科学院生态环境研究中心

甲烷是最重要的温室气体之一,目前对温室效应的贡献率约为20%。大气 CH的含量是由其源和汇二者共同决定的,源的增大和汇的减小均会使得大气 CH含量增加。土壤中甲烷营养菌对甲烷的氧化是大气甲烷唯一的生物汇。甲烷营养菌（Methanotroph... 详细信息

甲烷是最重要的温室气体之一,目前对温室效应的贡献率约为20%。大气 CH的含量是由其源和汇二者共同决定的,源的增大和汇的减小均会使得大气 CH含量增加。土壤中甲烷营养菌对甲烷的氧化是大气甲烷唯一的生物汇。甲烷营养菌（Methanotrophs）又称甲烷氧化菌,是一类以甲烷为唯一碳源和能源的功能微生物。甲烷营养菌可分为 Type Ⅰ和 Type Ⅱ两种类型。土壤甲烷营养菌的丰度可用来反映土壤对甲烷的吸纳能力,对于监测土壤对大气甲烷的消耗情况,寻求全球温室效应的缓解途径具有重要意义。本研究采用实时荧光定量 PCR（Real-time PCR）的方法测定了旱地红壤中甲烷营养菌总的 pmoA 基因（编码颗粒状甲烷单氧化酶）拷贝数,以及基于16S rRNA 基因的 Type Ⅰ、Type Ⅱ型甲烷营养菌拷贝数,以考查长期不同土地利用方式（>10年）对表层（0-10 cm）土壤甲烷营养菌数量的影响。试验处理为: （1）退化区（D,原始利用的自然植被演替）;（2）农作区（F,耕地利用,季节作物植被）;（3）湿地松（P,针叶林单一植被）;（4）恢复区（R,自然植被）;（5）茶园区（T,常绿灌丛单一植被）。定量结果显示,Type Ⅰ型甲烷营养菌数量:F>R>T>P>D;Type Ⅱ型甲烷营养菌数量:R >F>T>P>D;而甲烷营养菌的 pmoA 基因拷贝数大小依次为:R>F>P>T>D。恢复区与农作区的甲烷营养菌数量显著高于其它处理;湿地松与茶园区其次,且二者间无显著差异;而退化区土壤甲烷营养菌数量显著低于其它演替系统。五种不同利用方式下土壤营养菌数量均一致表现为 Type Ⅰ>Type Ⅱ,说明 Type Ⅰ型甲烷营养菌可能在该土壤甲烷氧化过程中起主导作用。相关性分析发现,甲烷营养菌 Type Ⅰ和 Type Ⅱ的总拷贝数与总的 pmoA 基因拷贝数呈显著正相关（r=0.845,n=15,P<0.01）,表明通过分别定量甲烷营养菌的16S rRNA 基因和 pmoA 功能基因拷贝数可获取相一致的结果,即旱地农作（季节作物植被）及自然植被（恢复区）条件下土壤具有相对较高的甲烷氧化能力;相反,单一植被条件下土壤甲烷氧化能力较低,而退化生态系统（每年收割地表植被并移出）土壤的甲烷氧化能力最低。总之,不同土地利用方式亦即地表植被不同对旱地红壤吸纳甲烷的能力具有重要的影响。

关键词：甲烷营养菌大气甲烷不同利用方式基因拷贝数

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Isolation and Characterization of Three Sphingobium *** Capable to Degrade Phenylurea Herbicide Isoproturon

Isolation and Characterization of Three Sphingobium *** Capa...

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第十次全国环境微生物学术研讨会

作者： Sun Jiquan Huang Xing Li Shunpeng Key Lab of Microbiological Engineering of Agricultural Environment,Ministry of Agriculture,College of Life Science,Nanjing Agricultural University,Nanjing,P.R.of China

Three strains of bacteria （designated as YBL1,YBL2,YBL3 respectively） capable to degrade isoproturon,3-（4-isopropylphenyi）-1,1-dimethylurea （IPU）,were isolated from sludge collected from the herbicide *** on partial analysis of the 16S rRNA gene,and Phenotypic and biochemical characterization,these strains were identified as Sphingobium *** of these strains, Sphingobium ***2,at inoculation quantity of 10～7 cells per liter was able to degrade 30 mg.L IPU provided as a sole source of carbon,nitrogen,and energy within 24 hours in mineral salt *** optimum condition for IPU degradation by these strains was pH 7.0,and temperature 30℃.The Mg (1 mmol·L) could enhance the IPU degradation rate,but Ni,Cu (1 mmol·L) inhibit IPU degradation *** three strains could remove the residue of the other phenylurea herbicide such as chlorotoluron and diuron in mineral salt medium and in the Magan *** soil pH was the most important factor for the degradation by these *** N-demethylation was the first step of degradation of dimethylurea-substituted herbicides isoproturon and *** these strains were also found capable of degrading Propail.

关键词： Isolation and Characterization of Three Sphingobium sp.Strains Capable to Degrade Phenylurea Herbicide Isoproturon

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Effect of carbon utilizing on nitrate reduction and nitrous oxide emission in denitrifying process

Effect of carbon utilizing on nitrate reduction and nitrous ...

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第十次全国环境微生物学术研讨会

作者： Li Ping Li Qiuhua Zhu Pingping Wu Jinhua Liang Shizhong School of environmental science and engineering,South China University of Technology,Guangzhou,P.R.China School of bioscience and bioengineering,South China University of Technology,Guangzhou,P.R.China

Nitrous oxide （NO） is an important greenhouse gas and has significant impact on the *** process of wastewater treatment is one of the important anthropogenic sources in atmosphere *** is an intermediate during denitrifying process which consumes organic substrates as electron *** study is intended to screen some optimal carbon substrates to suppress NO emissions and maintain the great denitrifying *** organic carbons including glucose,sucrose and sodium acetate were used to evaluate their effects on NO emission and nitrate reduction during the denitrifying process of *** results showed that the types of organic carbon substrates and the COD/N-NO ratio had significant influences on NO emission and nitrate *** denitrifying efficiency and NO emission increased as the COD/N-NO ratio *** optimal COD/N-NO ratio of the denitrifying potential for glucose,sucrose and sodium acetate was 7.59,8.11 and 4.6 *** this condition,nitrate removal rate were 98.8%,99% and 99%;total nitrogen removal rate were 90%, 77%,and 93.06%;their maximum NO emission concentrations were about 95μL L,78.5μL L and 7μL ***,sodium acetate was the optimal carbon substrate to suppress NO emission and to promote the nitrate reduction.

关键词： Nitrous oxide biological nitrogen removal organic carbon denitrifying process wastewater treatment

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High-purity nano particles ZnS Production by a simple coupling reaction process of biological reduction and chemical precipitation mediated with EDTA

High-purity nano particles ZnS Production by a simple coupli...

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第十次全国环境微生物学术研讨会

作者： Baoping Xin Qun Huang Xuemei Tang School of Chemical Engineering and Environment,Beijing Institute of Technology,Beijing 100081,People's Republic of China

High-purity nano particles ZnS has been successfully synthesized using a simple coupling reaction process of biological reduction and chemical precipitation mediated with EDTA referred to as CRBRCP-EDTA *** research investigated the optimum conditions of the transformation of SO into S by SRB and the production of ZnS in CRBRCP-EDTA *** results showed that the molar ratio of Zn to EDTA=1:1 was crucial for SRB growth and ZnS *** the ratio=1:1,lower Zn concentration enhanced both the growth of SRB and the reduction of SO,leading to higher ZnS *** increase in NaSO concentration resulted in decrease in both SRB growth and SO reduction, it improved the S and ZnS *** the optimum conditions （0.05 mol/L ZnCl, 0.05 mol/L EDTA and 0.1 mol/L NaSO）,the synthesized ZnS was characterized by X-ray diffraction （XRD）,energy dispersive X-ray （EDX）,scanning electron microscopy （SEM） and transmission electron microscopy （TEM）.The analysis showed that the obtained ZnS were high-purity and well-distributed solid spheres with diameters ranging from 15-30 nm for primary particles and around 400 nm for secondary *** polyacrylamide was incorporated in CRBRCP-EDTA process,the secondary particles's diameters were reduced to less than 100 nm.

关键词： ZnS SRB nanoparticle synthesis

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Characteristic and Phylogenetic Analysis of Facultative Anaerobic Dissimilatory Azoreducing Bacteria from Anaerobic Activated Sludge

Characteristic and Phylogenetic Analysis of Facultative Anae...

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第十次全国环境微生物学术研讨会

作者： Yiguo Hong,P Jun Guo,P Guoping SunP Guangdong Institute of Microbiology,Guangzhou 510070,People's Republic of China South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,People's Republic of China

Physiologically distinct facultative anaerobic microorganisms were isolated and investigated for their ability to oxidize different substrates with azo compound as a terminal electron acceptor. 4 strain dissimilatory azo-reducing bacteria (DARBs) were isolated from activated sludge of a textile-printing waste-water treatment *** of these isolates could grow using oxygen as a sole electron acceptor and could reduce azo compound by coupling oxidation of several of electron *** isolate was preferred to different electron donor for azoreduction,such as hydrogen,formate or *** relationships among these DARB were examined by phylogenetic analysis of 16S rDNA *** of the genera Citrobacter (AzoR-1), Acinetobacter (AzoR-3),and Pseudomonas (AzoR-9) formed a monophyletic group within the gamma subdivision of the class Proteobacteria,which was closely related to the members of previously described Shewanella genera that can obtain energy for growth by this dissimilatory azoreduction *** genus Bacillus (AzoR-6) make up a distinct branch within the Firmicutes *** results of this study significant increase the limited number of microbial isolates that are known to be capable of dissimilatory azo-reduction and demonstrated that the ubiquity of azoreduction may be as a result of the presence of phylogenetically diverse microorganisms,which be able to reduce azo compounds with hydrogen or some short-chain fatty as electron donor.

关键词： Dissimilatory azo-reducing bacteria (DARB) Azoreduction Phylogenetic analysis Redox mediator

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Modified fluorogenic model substrate (FMS) technique to measure extraceilular enzyme activities of mangrove swamp surface sediments

Modified fluorogenic model substrate (FMS) technique to meas...

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第十次全国环境微生物学术研讨会

作者： Hui-Jie Liu Yun Tian Tian-Ling Zheng Chong-Ling Yan Hua-Sheng Hong School of Life Sciences,MOE of Key Laboratory for Cell Biology and Tumor Cell Engineering Xiamen University,Xiamen 361005,China State Key Laboratory for Marine Environmental Sciences,Xiamen University,Xiamen,361005,China

Eight transects including sixteen stations were established in the Fugong mangrove (117°54′-117°55′E,24°22′-24°24′N) of the Jiulong River Estuary,Fujian,China.A physical, chemical and microbiological evaluation of sediments was made using measurements of routine physical and chemical properties,and bacterial *** distribution pattern of bacterial α-glucosidase and β-glucosidase activities was investigated using a fluorogenic model substrate (FMS) technique with modification in order to allow better understanding of in situ enzyme activities,as well as their relation to bacterial biomass,metabolic activity and environmental factors in mangrove *** results showed that the enzyme activities of α-glucosidase (10.83～100.86μmol/L/h) and β-glucosidase (39.60～222.75μmol/L/h) varied among the different stations,and the enzyme activities of β-glucosidase were higher than those of α-glucosidase at all *** extracellular enzyme activities were positively related to organic C,organic matter and bacterial *** results also suggest that,based on the activities of α-glucosidase and β-glucosidase,they may be the most important extracellular enzymes in mangrove *** addition,the use of the FMS technique to measure extracellular enzyme activities of mangrove sediments could help us to evaluate their catabolic behavior in situ and so lead to a better understanding of the polluted status of mangrove swamp ecosystems.

关键词： Fluorogenic model substrate α-glucosidase activities β-glucosidase activities Mangrove swamp

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Comparative research of anthraquinone,triphenylmethane and azo dyes decolorization by a newly isolated Bacillus cereus strain DC11

Comparative research of anthraquinone,triphenylmethane and a...

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第十次全国环境微生物学术研讨会

作者： Daiyong Deng Jun Guo Guoqu zeng Guoping Sun Daiyong Deng South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China Guangdong Institute of Microbiology,Guangzhou 510070,China China Graduate School of the Chinese Academy of Sciences,Beijing 100039,China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangzhou 510070,China Jun Guo,Guoqu zeng,Guoping Sun Guangdong Institute of Microbiology,Guangzhou 510070,China

A broad-spectrum dye-decolorizing and degradation bacterium,strain DC11,was isolated and identified as a member of Bacillus cereus from activated *** important parameters including pH,temperature,oxygen concentration and carbon sources were comparatively studied using anthraquinone,triphenylmethane,and azo dyes,*** color removal,the suitable pH and temperature were 6.0-8.0 and 20-45℃,*** than 95% of 100 μM Acid Blue 25 (anthraquinone dye) was anaerobic degraded within 8 *** than 95% of 750 μM Basic Blue X-GRRL (azo dye) was anaerobic decolorized within 2 *** strain could also decolorize 98% of the 55 μM Malachite Green (triphenylmethane dye) within 6 h under microaerophilic or anaerobic *** fraction decolorizing assays showed that the color removal of these dyes was contributed to the soluble cytosolic fraction,and these functional components showed NADH/NADPH-dependent *** products were analyzed with *** intermediate metabolite was presented before the anthraquinone bone breakage in Acid Blue 25 degradation;Malachite Green was degraded into 4,4'-bis(dimethylamino)benzophenone and benzophenone;and Basic Blue X-GRRL decolorization was mainly due to the azo bond breakage.

关键词： Bacillus Comparative research of anthraquinone,triphenylmethane and azo dyes decolorization by a newly isolated Bacillus cereus strain DC11

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Characterization of Plasmid pWM240 from Ochrobactrum anthropi KT-C024 and its Application as an Abroad Host Range Vector

Characterization of Plasmid pWM240 from Ochrobactrum anthrop...

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第十次全国环境微生物学术研讨会

作者： Wang Liying Qiao Lin Sha Shengde Cao Xiaojin Xia Mian 1,Beijing Wei Ming Kai Tuo Agriculture Biotechnology Co.,Ltd.Beijing,100085 2,Institute of Nuclear and New Energy Technology,Tsinghua University,Beijing,100084

pWM240,a plasmid with the size of 4,277 base-pair,was isolated from Ochrobactrum anthropi KT-C024,and its complete nucleotide sequence was *** analysis indicated a putative oriT site and 5 ORFs,including one replication protein (ORF1) and one mobilizing protein (ORF3) in *** of ssDNA intermediate in *** cells suggested pWM240 replicates via rolling-circle- replication (RCR).pWM241 was constructed by inserting a kanamycin resistance gene into pWM240 and pWM242 was constructed with an addition of green fluorescent protein gene into pWM241 in order to confer pWM240 to screening markers. The mutation of hypothesis mobilizing protein gene (ORF3) disrupted the conjugative function of pWM241,which indicated ORF3 endowed the plasmid with transferable characters,pWM241 was employed successfully to express cyanide-degrading gene cya-1 from Pseudomonas aeruginosa KT-C001,which showed the vector pWM241 has the potential in applying the gene enhanced technology (GET) into wastewater treatment,pWM242 had a broad host range and was stably maintained with antibiotic selection when transformed into Escherichia coli, Pseudomonas aeruginosa,Pseudomonas stutzeri,Paracoccus denitrificans and Ochrobactrum *** green fluorescent protein was expressed in these species too.

关键词： Ochrobactrum anthropi plasmid rolling-circle- replicating broad host range plasmid gene enhanced technology

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Impact of absence of sulfate or nitrate addition on bacterial populations and the end product patterns in a sulfidogenic bioreactor

Impact of absence of sulfate or nitrate addition on bacteria...

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第十次全国环境微生物学术研讨会

作者：赵阳国中国海洋大学崂山校区环境科学与工程学院

To investigate the functions of different microbial populations and corresponding changes of end product pattern in an acidogenic sulfidogenic bioreactor,the electron acceptor sulfate was eliminated for seven days and then the inhibiting agent of sulfate-reducing bacteria (SRB) nitrate was fed to the ***-strand conformation polymorphism (SSCP) profiles based on partial 16S rRNA genes of activated sludge and semi-quantifying for the denitrifying bacteria as well as routine chemical analyses were utilized in monitoring the whole period.(1) The bioreactor started up successfully and sulfate removal rate reached above 80% after about 20 days' operation. Different populations drastically competed and finally formed a stable dominant populations comprising of Desulfobulbus sp.,Desulfovibrio spp.,Clostridium *** Pseudomonas *** acetic acid was the main part of end products.(2)The absence of sulfate in influent influenced little on quantity and population structure of SRB,while more on other microbial *** propionic acid,but not acetic acid,became the main part of end *** *** charge in oxidizing propionic acid in presence of *** the reduced sulfate,79% of sulfate obtained electrons from the oxidization of propionic acid by Desulfobulbus *** the sulfate was fed renewedly and composition of influent resumed,the microbial community structure was not affected and sulfate removal rate quickly amounted to 80% without clear delay *** acetic acid became the main part of end products.(3) When nitrate was fed to the sulfidogenic bioreactor,sulfate reduction was suppressed and down to 60% of total sulfate, consequently oxidation-reduction potentials (ORP) increased from -410 mV to -392 mV,while nitrate removal rate along was kept above 99%.SSCP profiles showed that most of SRB were inhibited while the relative quantity of denitrifying bacteria ***,the reduced product of nitrate by the nitrate-reduc

关键词： Sulfate-reducing bacteria (SRB) Community structure Electronic acceptor Single-strand conformation polymorphism (SSCP)

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