检索结果-内蒙古大学图书馆

您好，读者！请登录

内蒙古大学图书馆

首页
概况
党建
资源
服务
科研支持
- 论文收录引用证明
- 科技查新
知识产权
档案馆
帮助

咨询与建议

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

您的常用邮箱：*

您的手机号码：*

问题描述：

当前已输入0个字，您还可以输入200个字

全部搜索
期刊论文
图书
学位论文
标准
纸本馆藏
外文资源发现
数据库导航
超星发现

高级检索

时间限定

出版年份：

文献类型

图书期刊文献学位论文多媒体

馆藏选择

电子馆藏纸本馆藏

核心期刊

全部期刊 SCI 收录期刊 SSCI 收录期刊 EI 收录期刊 CSCD 收录期刊 CSSCI 收录期刊

语言

中文英文

文献类型

期刊文献图书学位论文标准纸本馆藏

帮助

文字说明：

T=题名（书名、题名），A=作者（责任者），K=主题词，P=出版物名称，PU=出版社名称，O=机构（作者单位、学位授予单位、专利申请人），L=中图分类号，C=学科分类号，U=全部字段，Y=年（出版发行年、学位年度、标准发布年）

检索规则说明：

AND代表“并且”；OR代表“或者”；NOT代表“不包含”；(注意必须大写,运算符两边需空一格)

检索范例：

范例一：(K=图书馆学 OR K=情报学) AND A=范并思 AND Y=1982-2016
范例二：P=计算机应用与软件 AND (U=C++ OR U=Basic) NOT K=Visual AND Y=2011-2016

分类表

所选分类

>> <<

限定检索结果

文献类型

127 篇 会议

馆藏范围

127 篇 电子文献
0 种 纸本馆藏

日期分布

学科分类号

125 篇 农学
- 118 篇 畜牧学
- 4 篇 作物学
- 4 篇 兽医学
- 2 篇 林学
- 1 篇 植物保护
13 篇 工学
- 13 篇 生物工程
11 篇 理学
- 11 篇 生物学

主题

21 篇 猪
17 篇 遗传多样性
13 篇 pcr-rflp
12 篇 微卫星
9 篇 多态性
9 篇 鸡
8 篇 绵羊
7 篇 snp
7 篇 pcr-sscp
6 篇 牛
5 篇 单倍型
4 篇 单核苷酸多态性
3 篇 鸭
3 篇 肉质
3 篇 山羊
3 篇 遗传多态性
2 篇 核移植
2 篇 卫星dna
2 篇 mtdna控制区
2 篇 牦牛

机构

15 篇 中国农业科学院畜...
15 篇 华南农业大学
10 篇 西北农林科技大学
9 篇 华中农业大学
8 篇 中国农业大学
7 篇 中国农业科学院家...
7 篇 西南民族大学
6 篇 吉林大学
6 篇 扬州大学
5 篇 南京农业大学
5 篇 四川农业大学
5 篇 中国农业科学院北...
4 篇 湖南农业大学
4 篇 青海省畜牧兽医科...
4 篇 浙江大学
3 篇 中国农业科学院兰...
3 篇 河北农业大学
3 篇 莱阳农学院
2 篇 广东省农业科学院...
2 篇 吉林省农科院畜牧...

作者

10 篇 张细权
9 篇 马月辉
8 篇 陈宏
6 篇 钟金城
6 篇 关伟军
6 篇 樊斌
5 篇 高雪
5 篇 陈金宝
5 篇 许尚忠
5 篇 任红艳
5 篇 赵书红
4 篇 高玉时
4 篇 马志杰
4 篇 徐宁迎
4 篇 陈宽维
4 篇 李慧芳
4 篇 刘榜
4 篇 陈国宏
4 篇 柳小春
4 篇 雷初朝

语言

117 篇 中文
10 篇 英文

检索条件"任意字段=第十次全国畜禽遗传标记研讨会"

共 127 条记录，以下是121-130 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

Goose Microsatellite Enrichment from AFLP Fragments by Magnetic Beads

Goose Microsatellite Enrichment from AFLP Fragments by Magne...

引用

第十次全国畜禽遗传标记研讨会

作者： TU Yun-Jie, CHEN Kuan-Wei, Gao Yu-Shi, TANG Qing-Ping (Poultry Institute, Chinese academy of agricultural science, Yangzhou 225003, China)

Microsatellite enrichment from AFLP fragments by magnetic beads was introduced in this research. This method can obviate the tedious work of library construction which not only save time but also expense. The genomic DNA was converted into Pre-amplified AFLP fragments by using a few restriction enzymes combination and hybridized with biotin-labeled SSR probes. Then the hybrid mixture was used to incubated with magnetic beads coated with streptavidin. After washing to remove the non-SSR fragments, the eluted single-strand DNA which was cloned and sequenced was largely enriched for microsatellites. Primers can then be designed according to the sequence flanking the repeat motifs and used for polymorphism analysis. Fifty one primers had been developed from six Chinese indigenous goose breeds successfully. Thirty one of them were middle polymorphic better specific and did not link each other. The mean Polymorphism Information Content (PIC) of 6 goose breeds was 0.394-0.448. The highest was in the Shitou (0.448), and in the Yan was the lowest (0.394). The results showed that the 31 microsatellites had middle PIC. These 31 microsatellites could be used in the analysis of genetic diversity, construction of saturated maps, and in some cases, in marker-assisted selection. The whole experiment can be employed as a reliable option for any molecular laboratory to develop SSR markers.

关键词： microsatellites AFLP magnetic beads goose.

来源：评论

学校读者我要写书评

暂无评论

SNPs of GH Gene Characterized by PCR-SSCP Analysis and Search for Their Associations with Milk Yield in Goat

SNPs of GH Gene Characterized by PCR-SSCP Analysis and Searc...

引用

第十次全国畜禽遗传标记研讨会

作者： LAN Xian-Yong , CHEN Hong, PAN Chuan-Ying , LEI Chu-Zhao (College of Animal Science and Technology, Northwest A& F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, P. R. China Institute of Cellular and Molecular Biology, Xuzhou Normal University ,Xuzhou 221116, P. R. China)

Single nucleotide polymorphisms (SNPs) characterized by PCR-SSCP analysis in exon V of growth hormone (GH) gene for 180 goats from five goat breeds were investigated and their associations with milk yield of Xinong Saanen dairy goats were searched. The results showed that four SSCP patterns were observed in goat. In reference to goat GH gene sequence (GenBank No.D00476) as a control, SSCP pattern sequences characterized 14 SNPs, among which 4 codon mutations changed no amino acid variations. The establishment of relationships between SSCP patterns and SNPs and milk performance from Xinong Saanen dairy goats was attempted by a general mixed linear model. Significant statistical relationship between 3 SSCP patterns and milk yields was observed (P<0.05), and significant association of 4 SNPs with milk production traits was preliminary to be found (P<0.05 and P<0.01). Hence, we drew a preliminary conclusion that 4 SNPs characterized by PCR-SSCP patterns for exon V of GH gene exerted significant effects on milk performance, could be considered as molecular marker in MAS.

关键词： PCR-SSCP Single nucleotide polymorphisms (SNPs) GH gene Milk yield Association.

来源：评论

学校读者我要写书评

暂无评论

Evaluation of Genetic Variability and Genetic Distance Between Twelve Chinese Indigenous Chicken Breeds

Evaluation of Genetic Variability and Genetic Distance Betwe...

引用

第十次全国畜禽遗传标记研讨会

作者： Chen Kuan-wei ,Li Hui-fang, Yu Ya-bo, Tang Qing-ping , Gu Rang, Zhu Wen-qi (Institute of Poultry Science, Chinese Academy of Agriculture Science, Yangzhou 225003 College of Animal Science and Technology, Yangzhou University, Yangzhou 225009)

A total of 720 individuals of 12 indigenous chicken populations, geographically localized in South China were genotyped for 30 microsatellite markers to evaluate the genetic variation and genetic distance between populations. The all microsatellites were found to be polymorphic. Heterozygosity and Wright's F-statistics (FIS, FST and FIT) were calculated to determine the genetic variation. Mean values of FIS, FST and FIT per locus were -.0484, 0.083 and -0.305, respectively. Of the 30 microsatellite loci, number of alleles per locus (Na) and effective number of alleles per locus (Ne) ranged from 4 to 11 and 2.157 to 8.019, respectively. The average expected heterozygosity (HE) was 0.669, while the average observed Heterozygosity (HO) was *** polymorphism information content (PIC) have values between 0.560 and 0.641. Using Nei's standard distance, genetic distance (DA) calculated ranged between 0.088 (Guanxi Sanhuang vs. Nandan Yao) and 0.495 (Huiyang Beard vs. Zhangzhou Game). The topology of phylogenetic trees constructed showed general patterns of relationship and genetic differentiation among the indigenous populations studied. The results provided evidence of the applicability of microsatellite to determining the genetic relatedness among different Chinese indigenous chicken populations and evaluating genetic variations.

关键词： microsatellite chicken breeds genetic distance genetic diversity

来源：评论

学校读者我要写书评

暂无评论

Association of Polymorphisms in the Pit-1 Intron 5 with Growth Traits in Chinese Cattle

Association of Polymorphisms in the Pit-1 Intron 5 with Grow...

引用

第十次全国畜禽遗传标记研讨会

作者： YANG Dong-ying, CHEN Hong, LAN Xian-yong, ZHANG Zheng-feng, TANG Li-gang, ZHANG Liang-zhi, WANG Xin-zhuang, WANG Yi-min, NIU Hu (College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China The Research Center of Beef Cattle Engineering and Technology in Henan, Zhengzhou, Henan 450003, China)

The Pit-1 gene was studied as a candidate for genetic markers of growth traits in Chinese cattle in this study. The single-strand conformation polymorphism method was used to detecte polymorphism in the Pit-1 gene including intron 5. One single nucleotide polymorphism was found in intron 5 of Pit-1. The frequencies of alleles A/B in Nanyang cattle(NY), Qinchuan cattle(QC). Jiaxian Red cattle(JXR), Xizhen cattle(XZ), Luxi cattle(LX) and Holstein cow(H) populations were 0.444/0.556 , 0.477/0.523, 0.538/0.462, 0.421/0.579, 0.523/0.477, 0.475/0.525 respectively. Associations of the polymorphisms with growth traits in Nanyang cattle were analyzed using a general linear model procedure. The following parameters were greater in individuals with genotype AA than with genotype AB: body weight, average daily gain, body height, chest girth at 6, 12, 18 and 24 months(P<0.01). Body weight and body size also showed a trend of allele B > allele A in other age groups. Therefore, genotype BB maybe a dominant genotype and allele B may be a dominant allele. These results imply that allele B of Pit-1 gene likely positively affects growth traits.

关键词： Beef cattle, Growth traits, Pit intron 5, Polymorphism

来源：评论

学校读者我要写书评

暂无评论

Association with TGF-β1 gene polymorphisms and litter size in Large White

Association with TGF-β1 gene polymorphisms and litter size ...

引用

第十次全国畜禽遗传标记研讨会

作者： Y.P. WU, A.G Wang, N. Li, J.L. Fu (College of Animal Science and Technology, China Agricultural University, Beijing 100094 China Astronaut Research and Training Center, Beijing 100094 State Key Laboratory for Agro-Biotechnology, China Agricultural University, Beijing 100094)

As a multifunctional cytokine, the transforming growth factor β1 (TGF-β1) was detected in the utero-placental interface during early pregnancy in the pig, and believed to enhance trophoblast attachment to the endometrium. In this experiment, we selected TGF-β1 as the candidate gene affecting litter size in pigs. We detected the polymorphisms of this gene by PCR-SSCP (single-strand conformation polymorphism ) in Large white sows (n=567), four polymorphisms loci were found : C→T mutation at 33nt (GenBank NO: AF461809) in the intron 4, G→A mutation at 179nt (GenBank NO: AJ621785 )in the intron 6, C→T mutation at 1043nt (GenBank NO: AJ621785) in the intron 6, GG→AA linkage mutations at 2490nt and 2494nt respectively (GenBank NO: M23703)found in the 3' flanking region. We haplotyped these SNPs into two haplotypes: CGCAA (denote as P) and TATGG (denote as K). The effects of three haplotypic combinations of PP, PK and KK on litter sizes of first parity and second parity were estimated by Linear model, we found that for the first parity litters, the difference between KK and PK was 1.02 pigs per litter (P<0.05) for TNB, 0.67 pigs per litter but not significant (P>0. 1) for NBA, there were 0.49 pigs per litter and 0.37 pigs per litter but not significant difference between KK and PP for TNB and NBA respectively, a slightly difference of 0.53 pigs per litter between PP and PK were existed (0.05

关键词： TGF-β1 gene litter size SNP haplotypic combination

来源：评论

学校读者我要写书评

暂无评论

Association of Polymorphisms of IGF-1 and GHR with Reproductive Traits in Wenchang Chicken

Association of Polymorphisms of IGF-1 and GHR with Reproduct...

引用

第十次全国畜禽遗传标记研讨会

作者： CHEN Kuan-wei,WU Xu,LI Hui-fang , TANG Qing-ping,GAO Yu-shi, ZHU Wen-qi (Institute of Poultry Science, Chinese academy of agriculture science, Yanghou 225003 College of Animal Science and Technology, Yangzhou 225009)

Alleles of physiological candidate genes for reproductive traits, insulin-like growth factor-1 (IGF-1) and growth hormone receptor (GHR) were assessed to determine the association with the total egg production (NE), average days of continual egg-laying (ADCE) and number of double-yolked eggs (DYE) in Chinese indigenous breed chicken (Wenchang). PCR-RFLP method was used for genotypes identification. The frequency of restriction enzyme C1/ C2 alleles in the population was 0.53 (C1) and 0.47 (C2) for IGF-1. For GHR-Intron 2 was 0.06 and 0.94 for (A1) and (A2), respectively, while GHR-Intron5 was 0.20 (B1) and 0.80 (B2). IGF-1 polymorphism has an additive effect on egg production trait. The IGF-1 PstI C2C2 genotype had greater number eggs (300d) at 89.03 compared to 82.61 for PstI C1C1 (P<0,05) and (400d) at 137.84 compared to 127.82 for PstI C1C1 (P<0.05). The IGF-1 C2C2 genotype had longer ADCE at 3.38 compared to 2.96 and 2.78 for PstI C1C1 and C1C2 , respectively (P<0.05). GHR polymorphism has additive effect on number of double-yolked eggs (DYE), The GHR HindⅢ A1A1 genotype had greater DYE at 1.00 compared to 0.30 for HindⅢ A2A2 (P<0.05). The current research supports the effects of GHR and IGF-1 genes on reproductive traits of chickens.

关键词： Chicken IGF-1 GHR Reproduction SNP

来源：评论

学校读者我要写书评

暂无评论

Molecular Genetic Characterization Of MC4R Coding Region In Chinese Cattle Breeds

Molecular Genetic Characterization Of MC4R Coding Region In ...

引用

第十次全国畜禽遗传标记研讨会

作者： ZHANG Chun-lei, CHEN Hong, LAN Xian-yong, ZHANG Li, ZHANG Ai-ling (College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China)

Source/description Melanocortin-4 receptor (MC4R) is one of five protein-coupled receptors binding melanocortins that is implicated in the control of ingestive behavior and energy homeostasis. Mutations have been described in the human and mouse MC4R genes which are associated with obesity. Moreover, a mutation in porcine MC4R is associated with economically important traits in the pig. The SNPs reported in bovine MC4R coding region were specific to breeds. In present experiment over 95% of the coding region of MC4R was screened to detect the SNPs in predominantly cattle breeds of *** conditions The 3 primers of PI(275-764), P2 (485-959) and P3 (804-1126) were designed based upon the bovine MC4R sequence (GenBank accession no. AF265221). The 15 μl polymerase chain reaction (PCR) contained 100 ng of genomic bovine DNA, 10 pmol of each primer, dNTPs (200 μM), Tris-HCl pH 8.8 (20 mM), (NH4)2SO4 (50 mM), MgC12 (1.5 mM), and 0.50 U Taq DNA polymerase (TaKaRa, Dalian, China).The cycling protocol was 4 min at 95℃, 35 cycles of 94℃ for 45 min, Annealing 55-63℃ for 45 s, 72 ℃ for 1 min, with a final extension at 72℃ for 4 min. Single stranded conformation polymorphism (SSCP) Aliquots of 5μl of the PCR products were mixed with 5 μl of the denaturing solution (95% formamide, 25 mM EDTA, 0.025% xylene-cyanole and 0.025% bromophenol blue), heated for 10 min at 98℃ and chilled on ice. Denatured DNA was subjected to PAGE (80×73×0.75 mm) in TBE buffer (89 mM Tris-Borate;2 mM EDTA;pH 8.3) and constant voltage (200V) for 2.5 h. The gel was stained with silver nitrate as previous described *** nucleotide polymorphism (SNP) The PCR fragments from different SSCP patterns were cloned and sequenced. Only two SNPs 927C>T and 1069C>G (GenBank #AF265221) were detected in 509 unrelated cattle from five breeds. The mutations 647T>A, 727G>A, 747G>A were not detected in present populations. This confirmed that these SNPs were specific to breeds. Interestingly, the frequency (Table 1)

关键词： Molecular Genetic Characterization Of MC4R Coding Region In Chinese Cattle Breeds GenBank

来源：评论

学校读者我要写书评

暂无评论

没有更多数据了...

全选清除本页清除全部题录导出标记到“检索档案”

共13页 << < 4 5 6 7 8 9 10 11 12 13 > >>

检索报告对象比较合并检索0

隐藏清空

合并搜索

回到顶部

执行限定条件

内容：

评分：

请选择保存的检索档案：

请选择收藏分类：

订阅名称：

通借通还

温馨提示：

图书名称：

借书校区：

取书校区：

手机号码：

邮箱地址：

一卡通帐号：

电话和邮箱必须正确填写，我们会与您联系确认。

联系人：

所在院系：

联系邮箱：

联系电话：

内蒙古自治区呼和浩特市赛罕区大学西街235号邮编: 010021

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：