The emerging roles of microRNAs(miRNAs)and pulmonary epithelial cells in regulating the immune response against microbial invasion has attracted increasing attention in recent years,however,the immunoregulatory roles ...
The emerging roles of microRNAs(miRNAs)and pulmonary epithelial cells in regulating the immune response against microbial invasion has attracted increasing attention in recent years,however,the immunoregulatory roles of miRNAs in the pulmonary epithelial cells in response to mycobacterial infection has not been fully demonstrated.
本研究构建一种新型电化学免疫传感器用于检测H5N1亚型禽流感病毒。利用氧化石墨烯（GO）负载H5N1亚型禽流感病毒多克隆抗体（PAb-H5N1）及牛血清白蛋白（BSA）作为信号放大材料。结果表明:以PAb-H5N1-GO-BSA纳米复合物作为信号放大材料构建的电化学免疫传感器的灵敏度比不用此纳米复合物作为信号放大的高256倍。以PAb-H5N1-GO-BSA纳米复合物作为信号放大材料构建的电化学免疫传感器对H5N1亚型禽流感病毒的检测限为:2 HA unit/50μL,检测线性范围为:2～2-8 HA unit/50μL。此传感器特异性好,灵敏度高,在病原微生物快速检测领域具有良好的应用前景。
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