<正>石墨烯具有高导电性、薄而机械强度大的特性,但它的亲水性差。Russo和Passmore利用氢等离子体处理的方法改善了石墨烯的亲水性能,并将其以连续薄膜的形式铺设至微阵列碳支持载网表面(Russo and Passmore,2014a)。他们的结果发...
<正>石墨烯具有高导电性、薄而机械强度大的特性,但它的亲水性差。Russo和Passmore利用氢等离子体处理的方法改善了石墨烯的亲水性能,并将其以连续薄膜的形式铺设至微阵列碳支持载网表面(Russo and Passmore,2014a)。他们的结果发现样品漂移速率下降了20%,有效缓解电子束引发的样品漂移。但报道
徕卡提供全套电镜样品制备解决方案。冷冻电镜分辨率高,对样品的结构真实度要求极高。单颗粒投入冷冻可以帮助我们利用Cryo-EM来研究大分子颗粒,如蛋白质分子或病毒颗粒。高压冷冻技术则可以帮助我们利用EM或Cryo-EM来研究细胞或组织。并且,高压冷冻技术还可以实现玻璃化冷冻切片的Cryo-EM分析,即CEMOVIS(Cryo-Electron Microscopy of Vitreous Sections),从而实现直接观察近乎原始状态的生物样品,并做计算机三维重构。利用高压冷冻技术还可以实现光镜电镜联用技术,即CLEM(Correlative Light and Electron Microscopy),可以保存样品在真实的生理状态下光镜观察与电镜观察相结合。近期,徕卡最新推出冷冻光镜电镜联用技术,通过全程冷冻制样与冷冻光镜电镜观察技术,真正做到原位结构原生态光镜与电镜联用。
<正>Mediator complex plays an essential role in regulation of eukaryotic *** Saccharomyces cerevisiae core Mediator comprises 21 subunits,which are organized into three modules,termed"Head","Middle&qu...
<正>Mediator complex plays an essential role in regulation of eukaryotic *** Saccharomyces cerevisiae core Mediator comprises 21 subunits,which are organized into three modules,termed"Head","Middle",and"Tail".The previous
<正>Running a cryoEM facility never was a simple *** advances in CryoEM field makes it a more demanding than ever,to deliver CryoEM data for both quality and *** remains to be a very complex and challenging job from...
<正>Running a cryoEM facility never was a simple *** advances in CryoEM field makes it a more demanding than ever,to deliver CryoEM data for both quality and *** remains to be a very complex and challenging job from user management
<正>The mitochondrion is known as the"powerhouse of the cell",for it provide energy to cell by oxidative *** mitochondria are also dynamic organelles,they fuse and divide to keep the cellular *** diseases ...
<正>The mitochondrion is known as the"powerhouse of the cell",for it provide energy to cell by oxidative *** mitochondria are also dynamic organelles,they fuse and divide to keep the cellular *** diseases can be
<正>In the era of direct electron detectors,transmission electron microscopy(TEM)imaging of frozen-hydrated biological requires careful selection of imaging conditions,including exposure time,dose rate,magnificati...
<正>In the era of direct electron detectors,transmission electron microscopy(TEM)imaging of frozen-hydrated biological requires careful selection of imaging conditions,including exposure time,dose rate,magnification,acceleration energy,et *** this
<正>Neuronal synapses are key communication devices in brain *** the synapse,various subcellular components form highly ordered yet dynamic structure to carry out critical functions of information transmission and *...
<正>Neuronal synapses are key communication devices in brain *** the synapse,various subcellular components form highly ordered yet dynamic structure to carry out critical functions of information transmission and *** cryo electron
The most recent technical advancement of cryo-electron microscopy is revolutionizing structural *** the new generation of transmission electron microscope and direct electron counting device,we can now solve the struc...
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The most recent technical advancement of cryo-electron microscopy is revolutionizing structural *** the new generation of transmission electron microscope and direct electron counting device,we can now solve the structure of an
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