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Polypyrrole/biodegradablepolymer composite micro/nano fibrous nerve tissue engineering scaffolds

Polypyrrole/biodegradablepolymer composite micro/nano fibrou...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者：俞巧珍 College of Materials and Textile Engineering Jiaxing Key lab of Medical molecular biology Zhejiang experimental center of materials and textile engineering Jiaxing University

＜正＞Electrical signal is an attractive guiding cue for promoting axonal regeneration in nerve injury ***（PPy）has been recognized as a promising scaffold material to electrically stimulate neurons and nerve tissues for therapeutic purposes such as nerve tissue engineering ***,PPy is brittle,rigid,and nonbiodegradable,which makes it unsuitable for application alone in nerve regeneration[5].Therefore,a lot of polymers have been tested for use in the fabrication of PPy/polymer composite nerve

关键词： Polypyrrole Regenerated spider silk protein poly（L-lactic acid） Peripheral nerve regeneration Electrical stimulation

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MicroRNA 29 familyand Parkinson’s disease

MicroRNA 29 familyand Parkinson’s disease

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者： Xiaochen Bai Yilin Tang Mei Yu Lei Wu Fengtao Liu Zishan Wang Jinghui Wang Jian Wang 黄芳 The State Key Laboratory of Medical Neurobiology and the Institutes of Brain Science Shanghai Medical College Fudan University Department of Neurology Huashan Hospital Fudan University

＜正＞Parkinson’s disease is the second most common neurodegenerative disease,characterized by the progressive loss of dopaminergic neurons in the substantia nigra of the *** is still a lack of effective diagnosis and treatments for *** RNAs have been proved to play an important role in PD *** RNA29 family（mi R-29s）is including two gene clusters:mi R-29a/b1 and mi R-29b2/c,which

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m TOR cell signaling pathway contributes to the protective effects of ischemic postconditioning against stroke

m TOR cell signaling pathway contributes to the protective e...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者：谢嵘 Peng Wang Michelle Cheng Robert Sapolsky Xunming Ji Heng Zhao Department of Neurosurgery Huashan Hospital Fudan University Departments of Neurosurgery Stanford University Departments of Neurosurgery Stanford University Department of Neurosurgery Xuanwu Hospital Capital Medical School Departments of Biological Sciences Stanford University

Introduction:Ischemic postconditioning(IPost C)provides protective effect against focal ischemia and improves neurological *** the m TOR pathway is protective or detrimental in brain injury induced by stroke is controversial,and whether it is involved in the protective effects of ischemic postconditioning against stroke has not been *** report that m TOR contributes to neuronal survival in vitro,in a neuronal culture,as well as in vivo,in stroke brains receiving IPostC.

关键词： TOR m TOR cell signaling pathway contributes to the protective effects of ischemic postconditioning against stroke

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Cox2 inhibition ameliorates seizure susceptibility in ADLTE mice

Cox2 inhibition ameliorates seizure susceptibility in ADLTE ...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者： Lin Zhou Liang Zhou Lida Su Shenglong Cao Yajun Xie Ying Shen Department of Neurobiology Key Laboratory of Medical Neurobiology of Ministry of Health Zhejiang University School of Medicine Neuroscience Care Unit Second Affiliated Hospital of Zhejiang University School of Medicine Neuroscience Care Unit Second Affiliated Hospital of Zhejiang University School of Medicine

Autosomal dominant lateral temporal epilepsy(ADLTE)is an inherited epilepsy syndrome caused by mutations in Lgi1 *** has been shown that glutamatergic transmission is altered in Lgi1-knockout mice and increased seizures can be reduced by restoring Lgi1 ***,the underlying mechanism for ADLTE epilepsy is ***,we examined intrinsic excitability of pyramidal neurons in the

关键词： Cox2 inhibition ameliorates seizure susceptibility in ADLTE mice

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Tubastatin A, an HDAC6 inhibitor,alleviates stroke-induced brain infarction and functional deficits: potential roles of α-tubulin acetylation and FGF-21 up-regulation

Tubastatin A, an HDAC6 inhibitor,alleviates stroke-induced b...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者：王志菲上海中医药大学基础医学院

Histone deacetylase(HDAC)6 exists exclusively in cytoplasm and deacetylates cytoplasmic proteins such asα-***6 dysfunctionis associated with several pathological conditions in the central nervous *** study investigated the beneficial effects of tubastatin A(Tub A),a novel specific HDAC6 inhibitor,in a rat model of transient middle cerebral artery occlusion(MCAO)and an in vitro

关键词： FGF potential roles of Tubastatin A an HDAC6 inhibitor,alleviates stroke-induced brain infarction and functional deficits tubulin acetylation and FGF-21 up-regulation

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Pirt reduces bladder overactivity by inhibiting purinergic receptor P2X3

Pirt reduces bladder overactivity by inhibiting purinergic r...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者：高霄飞第二军医大学神经生物教研室

Pirt is a transmembrane protein predominantly expressed in peripheral neurons. However, the physiological and pathological roles of Pirt in hollow viscus are largely unknown. Here we show that Pirt deficiency in mice causes bladder overactivity. The density of α,β-me ATP-induced currents is significantly reinforced in Pirt-deficient dorsal root ganglion(DRG) neurons. Pirt and P2X3 receptor co-localize in bladder nerve fibres and heterologous Pirt expression significantly reduces P2X3-mediated currents. Pirt interacts with P2X3 through the N-terminal 14 amino-acid residues. TAT-conjugated PirtN14 peptide(PirtN14) is sufficient to inhibit P2X3 activation in bladder DRG neurons and to alleviate bladder overactivity in Pirt-/- mice. Pirt expression is decreased in the bladder of cyclophosphamide(CYP)-treated mice, a commonly used model of bladder overactivity. Importantly, PirtN14 administration reduces the frequency of bladder voiding and restores the voided volume of CYP-treated mice. Therefore, our results demonstrate that Pirt is an endogenous regulator of P2X3 in bladder function.

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The Effects of 2-Aminoethoxydiphenyl borate (2-APB) on Human KV1 Channel Currents

The Effects of 2-Aminoethoxydiphenyl borate (2-APB) on Human...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者：赵伟 Yuan Chen Chinese Herb Medicine Division The Nurturing Station for the State Key Laboratory of Subtropical Silviculture Zhejiang Agriculture and Forestry University

2-Aminoethoxydiphenyl borate(2-APB) is a syntheticcompound that was originally introduced as amembrane-permeable inhibitor of intracellular inositol1,4,5trisphosphate receptors,and has been used extensively as a modulatorof manykinds of ion transport *** revealed a complex mechanism of action for 2-APB and its analogs on ion channels. However, effects of 2-APB on voltage-gated potassium channels(KV) have never been reported. In this paper, we discovered that 2-APB could function as an inhibitor of several human KV1 channels. 2-APB could significantly block KV1.2, KV1.3, and KV1.4 in a dose-dependent manner with IC50 s of 310μM, 454μM, and 32.6μM, respectively. Alanine scanning was performed on the cavity region of KV1.4 channel in order to identify potential 2-APB binding sites. V549 A, T551 A, L554 A, and V558 A mutations could significantly attenuated 2-APB effects on Kv1.4 channel currents, whereas the otherchanges had no significant effects.

关键词： 2-APB channel blocker KV1 2 KV1 3 KV1 4 potassium channel

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Dynamic Network Connectivity Analysis on Stereo-Electroencephalography

Dynamic Network Connectivity Analysis on Stereo-Electroencep...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者： Mao Jun-Wei Ye Xiao-Lai Li Yong-Hua Liang Pei-Ji Xu Ji-Wen 张溥明 School of Biomedical Engineering Shanghai Jiao Tong University Department of Functional Neurosurgery Renji Hospital Shanghai Jiao Tong University

Accurate localization of the epileptogenic zone（EZ） is essential for the successful surgical treatment of the refractory focal epilepsy. The aim of the present study is to investigate whether a dynamic network connectivity analysis based on stereo-electroencephalography（SEEG） signals is effective in localizing the *** data were recorded from seven patients underwent presurgical evaluation for the treatment of refractory focal epilepsy, and the subsequent resective surgery gave the patients good outcome. The time-variant multivariate autoregressive model was constructed by Kalman filter and the timevariant partial directed coherence was computed, which was then used to construct the dynamic directed network of the epileptic brain. Three graph measures, in-degree, out-degree and betweenness centrality, were used to analyze the characteristic of the dynamic network and to find the important nodes in it. In all seven patients, the indicative EZ localized by in-degree and betweenness centrality were highly consistent to the clinical diagnosed EZ. However, the out-degree did not indicate significant difference between nodes in the network. In this work, the method based on ictal SEEG signals and effective connectivity analysis localized the EZs accurately. It suggested that in-degree and betweenness centrality may be better network characteristics to localize the EZs than out-degree.

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Fucoxanthin, a marine carotenoid, reverses scopolamineinduced cognitive impairments in mice and inhibits acetylcholinesterase in vitro

Fucoxanthin, a marine carotenoid, reverses scopolamineinduce...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者： Jiajia Lin Lin Huang Jie Yu Siying Xiang Jinrong Zhang Shan He Xiaojun Yan Wei Cui 王钦文 Ningbo Key Laboratory of Behavioral Neuroscience Zhejiang Provincial Key Laboratory of Pathophysiology School of Medicine Ningbo University School of Marine Sciences Ningbo University

Fucoxanthin, a natural carotenoid abundant in edible brown seaweeds, has been shown to possess anti-cancer, anti-obesity, and anti-diabetic effects. In this study, we report for the first time that fucoxanthin effectively protects against scopolamine-induced cognitive impairments in mice. In addition, fucoxanthin significantly reversed the scopolamine-induced increase of acetylcholinesterase(ACh E) activity, and decreased both choline acetyltransferase activity and brain-derived neurotrophic factor expression. Using an in vitro ACh E activity assay we discovered that fucoxanthin directly inhibits ACh E with an IC50 value of 81.2 μM. Molecular docking analysis suggests that fucoxanthin likely interacts with the peripheral anionic site within ACh E, which is in accordance with enzymatic activity results showing that fucoxanthin inhibits ACh E in a non-competitive manner. Based on our current findings and previous publications showing that fucoxanthin possesses anti-oxidative and anti-inflammatory activities, we anticipate that fucoxanthin may exhibit great therapeutic efficacy for the treatment of Alzheimer's disease by acting on multiple targets, possibly via inhibiting ACh E, decreasing oxidative stress, and attenuating inflammation in the brain.

关键词： fucoxanthin scopolamine acetylcholinesterase Alzheimer's disease cognitive impairments

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Ruxiang and Myrrh alleviated the neuropathic pain by modulation of TRPV

Ruxiang and Myrrh alleviated the neuropathic pain by modulat...

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长三角地区神经科学论坛2016暨第八次会员代表大会

作者： Hu Danyou Yang Niuniu Wang Changming Zhu Chan Yang Yan Geng Xiao Gu Luoying Yuan Xiaolin Yu Lei Yu Guang 唐宗湘 Nanjing University of Chinese Medicine

Ethnopharmacological relevance:Neuropathic pain was regarded as a main form of chronic pain condition that remains difficult to *** transient receptor potential vanilloid channel 1(TRPV1),which plays a crucial role in chronic sciatic nerve constriction injury(CCI)model induced neuropathic *** China,Ruxiang(Frankincense)and Myrrh are often used together on clinic in order to obtain a synergistic

关键词： Ruxiangand Myrrh TRPV1 Neuropathic pain CCI model Dorsal root ganglion

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