Aim Ferredoxin gene of Trichomonas vaginalis was induced to express in Exoli. Methods Antiserum against *** was generated in rabbits injected subcutaneously and intramuscularly with soluble antigen. Antibody titre was...
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Aim Ferredoxin gene of Trichomonas vaginalis was induced to express in Exoli. Methods Antiserum against *** was generated in rabbits injected subcutaneously and intramuscularly with soluble antigen. Antibody titre was determined with ELISA method. The recombinant prokaryotic plasmid pET3C-Fd was transferred into Exoli BL21(DE3). The ferredoxin was induced to express by IPTG. Results The polyclonal antibody obtained from rabbit had a titre of above 1:8000 and the antibody can be used as primary antibody of Western blot assay. The expressed protein in Exoli was identified by SDS-PAGE and Western blot Conclusion The ferredoxin was successfully expressed in Exoli.
Cryptosporidium baileyi from ostrich endogenous development occurred primarily in the bursa of Fabricious of *** plentiful global and subglobal cryptosporidia cling in the surface of epithelium *** transmission electr...
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Cryptosporidium baileyi from ostrich endogenous development occurred primarily in the bursa of Fabricious of *** plentiful global and subglobal cryptosporidia cling in the surface of epithelium *** transmission electron microscope results indicated,the type Ⅰtrophozoite contained one big nuleus,there was wide clearance between nuleus membrane and cytoplasm,no clear fooder organelle;But it and rough endoplasmic reticulum were rich in type Ⅱtypetrophozoite, and one wide band was seen between dense band and fooder *** were two kinds of schizont (type Ⅰmeronts contained eight merozoites,and type Ⅱmeronts contained four merozoites) , the distinct residual body in type Ⅰmeronts, there were plentiful polysaccharide granules in residual *** were no cryptosporidia in trachea of *** article was the first detailed report on the ultrastructure of endogenous developmaental stages of Cryptosporidium baileyi from ostrich in ostriches.
Trichinellosis is a serious zoonosis with a worldwide distribution and pork is still the most common source of human trichinellosis, vaccines are needed to control Trichinella spiralis infection in swine. The recombin...
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Trichinellosis is a serious zoonosis with a worldwide distribution and pork is still the most common source of human trichinellosis, vaccines are needed to control Trichinella spiralis infection in swine. The recombinant eukaryotic expression plasmid pcDNA3-TspEl contained the gene encoding a 31 kDa antigen of T. spiralis was constructed. BALB/c mice were immunized with plasmid DNA vaccine by intramusclar injection and gene-gun delivery. The transcriptional activity of the pcDNA3-TspEl in skin and muscles at the site of inoculation was detected by RT-PCR using specific primers. The expression of TspE1 gene in skin and muscles at the site of inoculation was detected by immunohistochemistry and indirect fluorescencent antibody test (IFAT), respectively. These results indicated that the recombinant plasmid pcDNA3-TspEl was successfully transcribed and expressed in skin and muscles at the site of inoculation of mice. Thus, the plasmid encoding 31 kDa antigen may be of value for further development of DNA vaccine against swine trichinellosis.
Aim To construct a eukaryotic cell expression recombinant plasmid containing ferredoxin gene of T. vaginalis. Methods Total DNA was extracted from T. vaginslis with Chelex-100 method and used as templates for PCR. The...
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Aim To construct a eukaryotic cell expression recombinant plasmid containing ferredoxin gene of T. vaginalis. Methods Total DNA was extracted from T. vaginslis with Chelex-100 method and used as templates for PCR. The Fd gene was directionally cloned into plasmid pMD-18T simple vector, then subcloned into eukaryotic expression vector pcDNA3.1(+).The transformants were screened and identified by PCR and restriction analysis. Results The size of amplified Fd gene was 306bp. The correct recombinant plasmid pcDNA3.1(+)-Fd was isolated and confirmed by PCR and restriction analysis. The DNA sequence of cloned gene was the same as the published sequence. Conclusion Recombinant plasmid are going to be used further study of function of the Fd.
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