Mobile genetic elements (MGEs) are DNA sequences that have the ability to integrate into the genome at a new site. MGEs exist in all plant and animals, and have driven genomes evolution in diverse ways. LTR (long term...
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Mobile genetic elements (MGEs) are DNA sequences that have the ability to integrate into the genome at a new site. MGEs exist in all plant and animals, and have driven genomes evolution in diverse ways. LTR (long terminal repeat) retrotransposons as one of GMEs can increase copies in the genome through RNA proceed. It is useful for molecular epidemiology research. By the sequence of LTR retrotransposon CsRn1, we used PCR method to amplify blanking regions between two retrotransposons by primers LTR1 + LTR2 and region of retrotransposon by LTR1 + MD2. RAPD-PCR is another useful tool for interspecies analysis. From the samples collected in two regions, we can find individual differences between worms, and cladograms were made by the result. It shows that the evolution in south china is much faster than the one in north. Also, comparing to RAPD-PCR, MGE-PCR is more sensitive and display more details between individual.
IL-17 cDNA of chicken was successfully amplified by RT-PCR in the study. Materials were derived using the following two approaches. In one method, the total RNA was isolated from chicken spleen lymphocytes stimulated ...
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IL-17 cDNA of chicken was successfully amplified by RT-PCR in the study. Materials were derived using the following two approaches. In one method, the total RNA was isolated from chicken spleen lymphocytes stimulated by ConA for 12h, 24h, and 48h respectively. In the second approach the lymphocytes of caeca tonsil and intraepithelial lymphocytes (IELs) from chicken that had been infected 2 times with Eimeria tenella sporulated oocysts (about 3.6×104/per chicken) were used for isolating total RNA The PCR products were then cloned into pMD18-T vector for sequencing. The gene ORF was 507 bp, and it matched with the predicted size. Compared with the published sequence in GenBank (AJ493595), the homology of nucleic acids and amino acids were 99.4% and 82.4% respectively. The gene was later sub-cloned into an expressing vector, designed as pET32a-IL-17. After induction by IPTG, the IL-17 gene was expressed and the fused protein was about 36kD.
Aim To study the antigenicity of frozen sections of Trichinella spiralis muscle larvae after storage at -20℃for different time. Methods The frozen sections of T. spiralis muscle larvae stored at -20℃for 5, 8 and 12 ...
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Aim To study the antigenicity of frozen sections of Trichinella spiralis muscle larvae after storage at -20℃for different time. Methods The frozen sections of T. spiralis muscle larvae stored at -20℃for 5, 8 and 12 years were used in indirect fluorescent antibody test (IFAT) to detect the anti-Trichinella antibodies in sera from patients with trichinellosis(37 cases), cysticercosis(12 cases), hydatidosis(10 cases), paragonimiassis(16 cases)and healthy persons(15 cases). Results All sera from 37 cases with trichinellosis were anti-Trichinella antibody positive when the antigen slides stored at -20℃for different time were used, there was no obvious difference in fluorescence among the antigen slides stored for different time. All of sera from cases with cysticercosis, hydatidosis and healthy persons were negative. Two out of 16 portion of sera from case with paragonimiasis were false positive reaction. Conclusion The frozen sections of T. spiralis muscle larvae could be stored -20℃for as long as 12 years without loss of their antigenicity.
Aim To isolate and purify antibacterial peptides from housefly larvae secretion and study some of their antibacterial activity. Method Antibacterial peptides were salted-out by Ammoium Sulfate、filtrated through sepha...
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Aim To isolate and purify antibacterial peptides from housefly larvae secretion and study some of their antibacterial activity. Method Antibacterial peptides were salted-out by Ammoium Sulfate、filtrated through sephadex chromatography and purificated with RP-HPLC. The antibacterial activity of the peptides were tested against Escherichia coli 4113 and some other medical bacteria with plate methods,. Results After ammonium sulfate salting-out, Sephadex G-100 and then G-50 filtration, one elution fraction showed potent against Escherichia coli 4113. After the RP-HPLC purification of the active fraction, three antibacterial fractions were collected at 6min, 19min, 20.5min. The concentration of antimicrobial peptides decreased but the antibacterial activity increased during the course of purification and they also showed potent against Pseudomonas aeruginosa(ATCC25853), Staphylococcus aureus (26112),Bacillus subtilis (63501-25). Conclusion There were three antibacterial fractions in housefly larvae secretioa The antibacterial activity of the peptides was increasing with the course of purification and the peptides showed potent against several Gram-negative and Gram-positive bacteria.
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