Ineffectiveness of anticancer drugs is frequently observed in cancer *** resistance of tumor cells to various cytotoxic drugs is defined as multidrug resistance(MDR).The purpose of our present study was to investigate...
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Ineffectiveness of anticancer drugs is frequently observed in cancer *** resistance of tumor cells to various cytotoxic drugs is defined as multidrug resistance(MDR).The purpose of our present study was to investigate the inhibit effects of L1EPO synthesized by our group on P-glycoprotein(P-gp)-mediated multidrug resistance(MDR)in K562/A02 *** the cytotoxicity of the compound and its ability to inhibit the K562/A02 cells were determined with SRB *** apoptosis was detected by Hochest33342 staining ***-PCR was used to detect mdr-1 gene transcription and the Western blot assay was used to assess P-gp ***,We found K562/A02 cell line was rendered resistant toward Adriamycin but not towards L1EPO when compared with the parental *** L1EPO could down regulated mdr-1 gene,and reduced the expression of P-gp and displayed a perfect dose ***,it had less cytotoxicity in human normal cell lines(fibroblast,VEC),GI50>10μmol/***,L1EPO has the potential to overcomes P-glycoprotein-mediated Multidrug Resistance in K562/A02 Cell Line.
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