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检索条件"任意字段=2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展"

共 48 条记录，以下是41-50 订阅

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Engineering of PEGylated Camptothecin into Core-shell Naonoparticles for Improving Its Solubility,Stability and Combinational Delivery

Engineering of PEGylated Camptothecin into Core-shell Naonop...

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： Feng Yue,Ren Tianbin,Li Yongyong,Ren Jie School of Materials Science and Engineering,Tongji University,1239 Siping Road,shanghai 200092,China.

Objective:We proposed to conjugate CPT to hydrophilic Poly(ethly glycol)(PEG),then to prepare a novel kind of core-shell nanoparticle from PEGylated CPT prodrug to improve the stability and solubility of CPT and be po... 详细信息

关键词： Engineering of PEGylated Camptothecin into Core-shell Naonoparticles for Improving Its Solubility,Stability and Combinational Delivery

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Aligned ultrafine fibers by stable jet electrospinning

Aligned ultrafine fibers by stable jet electrospinning

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： YUAN Huihua,ZHAO Shifang,LI Qin,LI Biyun,TU Hongbin,ZHANG Yanzhong~* College of Chemistry,Chemical Engineering & Biotechnology,Donghua University,Shanghai 201620,P.R.China.

Electrospinning has been widely recognized as a novel platform technique to construct biomimicking scaffolds for tissue ***,there is a huge technological challenge in making aligned fibers and structures applicable to certain *** formulating the viscoelasticity of spinning dopes and supressing the jet bending instability,we currently demonstrated the feasibility of making single fibers,fiber yarns,and unidirectional fiber membranes with fiber fineness of a few microns in *** the WAXD and polarized Fourier transform infrared spectra results suggested that stable jet electrospinning encouraged polymer chain and crystalline orientation,which may contribute to significant improvement in the mechanical properties of thus made ultrafine fibers,as confirmed by the tensile *** jet electrospinning would enable us to biomimickingly engineer tissues like tendon or ligament,where fiber alignment,fineness,and mechanical performance are the major concerns while constructing such specific tissue engineering scaffold.

关键词： CTS Aligned ultrafine fibers by stable jet electrospinning

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Electrospun HAp/CTS nanoflbrous scaffold as an inductive substrate for the osteogenic differentiation of mBMSCs

Electrospun HAp/CTS nanoflbrous scaffold as an inductive sub...

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： PENG Hongju,LI Biyun,TU Hongbin,LI Qin,ZHAO Shifang,ZHANG Yanzhong~* College of Chemistry,Chemical Engineering & Biotechnology,Donghua University,Shanghai 201620,P.R.China.

Developing novel artificial matrices or scaffolds that can mimic the stem cell living niche represents a very promising approach to manipulating the differentiation of stem cells in regenerative *** study aims at determining the efficacy of using biomimetic nanoflbrous scaffolds of hydroxyapatite/chitosan(HAp/CTS) to induce mouse bone marrow stem cells(mBMSCs) to differentiate into osteogenic lineage in vitro,in the absence or presence of an osteogenic supplementation of ascorbic acid,(-glycerol phosphate,and *** electrospun chitosan nanoflbrous scaffolds as a control,it was found that the expression of bone-specific genes was significantly higher in mBMSCs growing on HAp/CTS than those on CTS alone scaffolds in both normal and osteogenic *** phosphatase staining and collagen assay confirmed that the nanoflbrous HAp/CTS scaffolds stimulated mBMSCs differentiation into the osteoblastic *** results suggest that the electrospun nanoflbrous HAp/CTS scaffolds are osteoconductive and osteoinductive,enabling to regulate the osteogenic commission of the mesenchymal stem cells.

关键词： Electrospun HAp/CTS nanoflbrous scaffold as an inductive substrate for the osteogenic differentiation of mBMSCs CTS HAP

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Improved phase homogeneity in electrospinning biomimetic composite nanofibers of gelatin/PCL

Improved phase homogeneity in electrospinning biomimetic com...

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： FENG Bei,TU Hongbin,LI Biyun,ZHAO Shifang,LI Qin,ZHANG Yanzhong~* College of Chemistry,Chemical Engineering & Biotechnology,Donghua University,Shanghai 201620,P.R.China.

Electrospun gelain/PCL hybrid is a well-liked nanofibrous scaffolding material that have been widely used for biomimetically engineering a variety of ***,dissimilarity of the constituents in their physicochemical attributes would give rise to phase seperation within the fibrous *** study aims to 1) investigate the influence of phase seperation on the morphology,homogeneity,mechanical properties of the electrospun gelatin/PCL fibers,and 2) improve the phase homogeneity of electrospun gelatin/PCL nanofibers by using acetic acid(HAc).Our results showed that addition of a tiny amount(0.1%) of HAc remarkably improved the miscibility of gelatin and PCL which were dissolved in a common solvent of trifluoroethanol,and resulted in the formation of homogeneous and performance -optimized nanofibers upon *** culture experiment intended for skin tissue engineering indicated that thus modified electrospun gelatin/PCL scaffolds supported better cellular responsive behaviour.

关键词： Improved phase homogeneity in electrospinning biomimetic composite nanofibers of gelatin/PCL

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Electrospun 3-D macroporous nanoflbrous scaffolds from controllable patterning for cellular infiltration

Electrospun 3-D macroporous nanoflbrous scaffolds from contr...

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： Shifang Zhao,Huihua Yuan,Bei Feng,Hongju Peng,Yanzhong Zhang ~* College of Chemistry,Chemical Engineering & Biotechnology,Donghua University,Shanghai 201620,P.R.China.

Electrospinning is an efficient means to produce nanofibrillar and porous scaffolds that resemble the native extracellular ***,one of the noted problems with electrospun scaffolds is that the inherently tiny pores do not promote adequate cellular infdtration and tissue *** address this challenge,we herein develop electrospun three-dimensional(3-D) macroporous nanofibrous scaffolds from designing and employing particular non-conductive template collectors that contain patterned topographical *** was found that surfacce pattern of non-conducting collector could be accurately replicated by the electrospun *** similar experimental observations suggested that surface topography of collector is the key factor that determine the deposition and the arrangement of electrospun fibers in an electrospinning process,rather than electric *** finding enabled us to use different collectors with patterned microstructure to increase the pore size of electrospun *** pore size and the porosity were highly enlarged,depending on the collector patterns.

关键词： Electrospun 3-D macroporous nanoflbrous scaffolds from controllable patterning for cellular infiltration

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The study of bone tissue and vessels constructed by bidirectional induced bone marrow mesenchymal stem cells of rabbits in vitro

The study of bone tissue and vessels constructed by bidirect...

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： Guo Shangchun~1,Chen Xin~2,Wang Jinwu~(3*),Rui Biyu~2,Sun Hui~2,Dai Kerong~3 I.Shanghai Institue for Microsurgery of Extermities,The Sixth People's Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200233,China 2.Department of Orthopedics,The Sixth People's Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200233,China 3.Department of Orthopedics,The ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200011,China

Objective To study the ability of construction of bone tissue and vessels in vitro by the co-culture system including endothelial cells,osteoblasts and bone marrow Mesenchymal stem cells(BMSCs) from BMSCs bidirectional *** BMSCs were isolated from bone marrow by density gradient centrifugation. The adhesive cells were preserved to passage in *** cells were divided into four groups:group A(pure

关键词： Bone marrow mesenchymal stem cells Co -culture system In vitro angiogenesis assay Bidirectional induce

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Pluronic F127 Based Nano-micelles modified with Nuclei Localized Signals PKKKRKV Functionality for Drug Delivery

Pluronic F127 Based Nano-micelles modified with Nuclei Local...

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： Li Lan,Ren Tianbin,Li Yongyong,Ren Jie School of Materials Science and Engineering,Tongji University,1239 Siping Road,Shanghai 200092,China

Pluronic copolymers are composited with hydrophilic block poly（ethylene oxide）(PEO) and hydrophobic poly（propylene oxide） PPO in a triblock structure *** F127 is specified as ***,several articles have been published to illustrate the targeting modification of the F127 triblock copolymer in drug delivery ***,most successful anticancer drugs like doxorubicin（DOX） would react with DNA to induce the tumor *** many researchers have been focused on the nuclei functional molecular *** well known,PKKKRKV,the minimum nuclear localization signals（NLS） of SV40T antigen is a 7-mer peptide which will bind to the nuclei transport proteins importin a to target the nuclei *** has been extensively researched both in drug and gene delivery system considering the overcoming of the nuclei membrane barrier and the aggregating in the *** we were studied the target properties of the PV-7 covalently conjugating on the F127 block polymer to package the hydrophobic anti-cancer drug DOX.

关键词： Pluronic F127 Based Nano-micelles modified with Nuclei Localized Signals PKKKRKV Functionality for Drug Delivery

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Cytotoxicity and Apoptosis of Inorganic nanoparticles in human cancer cells

Cytotoxicity and Apoptosis of Inorganic nanoparticles in hum...

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： Jiangchao Qian ~(1,a),Yuan Yuan~(2,3,b),Changsheng Liu~(1,2,3,c,*),Wei Tang ~(1,d),Xun Lu ~(2,3,,e) 1.State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,PR China 2.Key Laboratory for Ultrafine Materials of Ministry of Education,East China University of Science and Technology,Shanghai 200237,PR China 3.Engineering Research Center for Biomedical Materials of Ministry of Education,East China University of Science and Technology,Shanghai 200237,PR China

In the past few years,the application of nanoparticles has been extended to biomedical and biotechnological fields,such as cancer therapy[1],medical imaging[2],drug carriers[3].In this study, cytotoxicity of three kinds of inorganic nanoparticles to cancer cells and the related mechanism were ***-shaped hydroxyapatite nanoparticles（HAPNs） with an average size of about 20 50 nm were synthesized by the aqueous precipitation *** anti -tumor activity,apoptosis -induction and cellular uptake of HAPNs in human gastric cancer（MGC80-3）,cervical cancer（HeLa）,hepatic cancer（HepG2） and normal hepatic（L -02） cells,were *** results showed that HAPNs significantly inhibited cell proliferation and induced apoptosis of MGC80-3,HepG2 and HeLa cells,but had no impact on L-02 *** anti-tumor activity and HAPN-induced apoptosis strongly depended on the type of cells,and decreased in the order of MGC80-3 > HepG2 > *** inhibiting action was related to the increased caspase-3 *** cellular internalization and biolocalization of the FITC-labeled HAPN showed that,after 48 h incubation,much more HAPNs were presented inside the nucleus of MGC80-3,suggesting that the nuclear trafficking of the particles into the nucleus may have predominant effect on the HAPN-induced *** nanoparticles （SNPs） within 7～50 nm also showed anti-tumor activity,and the efficacies of the anti-proliferation decreased in the order of 20 nm >7 nm >50 nm,a mitochondrial-dependent apoptosis pathway triggered by ROS -mediated oxidative stress may be involved in the SNPs -induced cytotoxicity in HepG2 *** case of realgar nanoparticles（RNs）,the RNs（127 nm ） may provide strong anti-proliferation effect on HepG2 cells and human osteosarcoma cell lines *** conclusion,all three kinds of nanoparticles showed considerable cytotoxicity to human cancer cells tested,but the mechanism involved may be different for various kinds of nanoparticles.

关键词： Hydroxyapatite nanoparticles SiO2 nanoparticles Realgar nanoparticels Cancer cells Cytotoxicity Apoptosis Cellular uptake Oxidative Stress

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