This study investigated the effect of heat stress on primary myocardial cell damage,histopathological lesions,apoptosis and it's signal transduction molecules and the expression of Hsp90αprotein and its corarespo...
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This study investigated the effect of heat stress on primary myocardial cell damage,histopathological lesions,apoptosis and it's signal transduction molecules and the expression of Hsp90αprotein and its coraresponding mRNA in response to heat stress in *** inc ubated at 37℃for 72 h in humidified atmosphere of 5%CO and 95%air,the myocardial cells were suddenly heat stressed for 10 min,20 min,40 min,1 h,2 h,4 h,6 h and 8 h in another incubator with 95%air and 5%CO at 42℃.Levels of CK enzyme,histopathology,apoptosis,apoptosis signal transduction and hsp90αwere detected using enzymatic assay,histopathological technique,flow cytometry and western blot *** levels were gradually changed during heat stress,suggesting that the myocardial cells were distinctly stressed and obviously damaged by the heat *** results of flow cytometry showed that apoptotic levels at 40 min and 8 h of heat stress were significantly higher than those of the control（P<0.01 and P<0.05） respectively. Levels of cytochrome c and cleaved caspase-3 significantly increased at 40 min heat *** 40 min heat stress,hsp90αshow significant *** results demonstrate that apoptosis inhibit hsp90α.In contrast,Hsp90αsignificantly increased（P<0.01） at 6 and 8 h heat *** elevation of hsp90αin the primary myocardial cells is important marker at the later time of the heat shock and may act as a protective protein.
The study was to map the linear B-cell epitopes on Eastern equine encephalitis virus(EEEV) E2 glycoprotein by *** preparation of recombinant EEEV E2 glycoprotein was according to the instructions of the Bac-to-Bac Bac...
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The study was to map the linear B-cell epitopes on Eastern equine encephalitis virus(EEEV) E2 glycoprotein by *** preparation of recombinant EEEV E2 glycoprotein was according to the instructions of the Bac-to-Bac Baculovirus Expression System and Ni-nitrilotriacetic acid affinity chromatography was used for the *** purified recombinant EEEV E2 protein was used as antigen for the generation of E2-specific pAbs and *** E2 protein-reactive antibody titers of the final immunization demonstrated that the titer of pAbs was over 1:10～6 and 51 hybridomas stably secreting mAbs against E2 protein were developed by indirect ELISA and *** IFA with Sf9 insect cells infected with BACV-E2 showed that all the 51 mAbs could react positively with EEEV E2 protein whereas no reaction was detected on the Sf9 cells infected with wild type ***,the IFA with BHK transfected with pShuttle-E2 demonstrated a different result that 45 mAbs could react positively with EEEV E2 protein while no reaction was found on other 6 *** heavy chain subtypes of 51 mAbs contained IgG1,Ig2a and IgM with only one type light chain,a k light chain. With 42 expression fusion polypeptides with MBP-tag,the result of WB showed that anti-E2 protein murine pAbs could react positively with 15 of the 42 polypeptides and negatively with MBP-tag,that is,15 linear B-cell epitopes of E2 protein through screening with murine pAbs were *** corresponding locations of these epitopes were at 1-16aa,11-26aa,101-116aa,11l-126aa,131-146aa, 151-166aa,171-186aa,201-216aa,231-246aa,24l-256aa,271-286aa,301-316aa,311-326aa,321-336aa, *** further identification,the above 15 polypeptides containing 16 amino acids were synthetized and the peptides ELISA confirmed that the murine pAbs had good reactivity with the above 15 synthetized polypeptides. To identify the epitopes against the prepared mAbs,the ELISA was carried out coating with the 42 expressed polypeptides.9 lin
作者：
YIN JingFengTANG QingWANG FengLongWANG JinLingDING YuLinTAO XiaoYanLI HaoSONG MiaoGUO ZhenYangSHEN XinXinLIANG GuoDongCollege of Veterinary Medicine
Inner Mongolia Agricultural UniversityHuhhot010018Inner MongoliaChina Key Laboratory for Medical VirologyMinistry of HealthInstitute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(IVDCChina CDC)Beijing 102206China State Key Laboratory for Infectious Disease Prevention and ControlInstitute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(IVDCChina CDC)Beijing102206China
Objective We performed pathological observation and etiological identification on specimens collected from cow, cattle and dog which were suspected of rabies in Inner Mongolia, in 2011, and analyzed their etiology and...
Objective We performed pathological observation and etiological identification on specimens collected from cow, cattle and dog which were suspected of rabies in Inner Mongolia, in 2011, and analyzed their etiology and characteristics. Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens. Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronai cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate. Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which lead to a different etiology characteristics of the epidemic situation.
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