ckground：The distribution of Toxoplasma gondii genotypes varies across the *** population structure studies revealed that a few major clonal lineages of T. gondii predominated in different geographical *** in South A...
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ckground：The distribution of Toxoplasma gondii genotypes varies across the *** population structure studies revealed that a few major clonal lineages of T. gondii predominated in different geographical *** in South America is genetically and biologically divergent，whereas this parasite is a remarkable clone in North America and Europe with a few major lineages including Types Ⅰ，Ⅱ and Ⅲ．Information on genotypes and mouse virulence of T. gondii isolates from China is SCarce and insufficient to investigate its population structure，evolution，and ***／Principal Findings：We analyzed 23 *** isolates from different hosts in this study(four from human and nineteen from cats)．Genotyping of these isolates using 10PCR-restriction fragment length polymorphism(PCR-RFLP)markers(SAG1，SAG2，SAG3，BTUB，GRA6，c22．8，c29-2，L358，PK1 and Apico)from DNA obtained from tachyzoites and／or cysts revealed five genotypes；among them three genotypes were atypical and two were *** atypical genotypes were designated as Chinese 1(ToxoDB＃9)，ToxoDB＃204，and ToxoDB＃205，*** strains belong to the Chinese 1 lineage，which Was previously reported as a widespread lineage from swine，cats，and human in *** human isolates fall into the type Ⅰ and Ⅱ lineages and no type Ⅲ isolates were *** remaining isolates belong to two new atypical genotypes which were analyzed for the first time in *** results show that these genotypes of *** isolates are intermediately to highly virulent in mice except for the stmin TgCtwh6，which caused parasitemia for 35 days post infection in mice although it Dossesses the uniform Chinese 1 ***，phylogenetic network analYses of all isolates of genotype Chinese 1 are identical，and there is no variation based on the 5equence data generated at four introns(EF1，HP2，UPRT1 and UPRT7)and two dense granule proteins GRA6 and ***／Significance：A limited genetic diversity
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