Objective:To explore influence of ethanol on the reflex time and absolute refractory period to ***:We inject the alcohol to the toad's abdominal lymph sac,respectively,to make blood concentration is 40mg/100ml,80m...
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Objective:To explore influence of ethanol on the reflex time and absolute refractory period to ***:We inject the alcohol to the toad's abdominal lymph sac,respectively,to make blood concentration is 40mg/100ml,80mg/100ml,120mg/100ml and reserve,using probes penetrate the cranial cavity from the foramen magnum,destroying brain tissue tissue in order to
Vascular endothelial cells(ECs) are exposed to different flow patterns,i.e.,disturbed ***, and the associated oscillatory shear stress(OSS) *** shear stress(PSS) lead to differential *** investigated the roles of clas...
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Vascular endothelial cells(ECs) are exposed to different flow patterns,i.e.,disturbed ***, and the associated oscillatory shear stress(OSS) *** shear stress(PSS) lead to differential *** investigated the roles of classesⅠandⅡhistone deacetylases(HDAC-1/2/3 and HDAC-5/7,respectively) in regulating NF-E2-related factor-2(Nrf2) and Kruppel-like factor-2
＜正＞Objectives Ang-（1-7） is generated from angiotensin 1（AngⅠ） and angiotensin 2（AngⅡ） under the catalysis of angiotensin converting enzyme（ACE） and angiotensin converting enzyme 2（ACE2）. Enhancing the e...
＜正＞Objectives Ang-（1-7） is generated from angiotensin 1（AngⅠ） and angiotensin 2（AngⅡ） under the catalysis of angiotensin converting enzyme（ACE） and angiotensin converting enzyme 2（ACE2）. Enhancing the effect of Ang-（1-7） to rebalance Ang-（1-7） and AngⅡis an effective strategy for treatment of *** aim of this study was to determine the role of ACE2/Ang-（1-7）/Mas
Understanding intracellular signaling mechanisms underlying protective response against ischemic injury in cardiovascular system is one of major steps in the development of effective drugs for treating ischemic ***,we...
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Understanding intracellular signaling mechanisms underlying protective response against ischemic injury in cardiovascular system is one of major steps in the development of effective drugs for treating ischemic ***,we have demonstrated using endothelium-specific
Hypoplastic left heart syndrome(HLHS) is a serious congenital cardiovascular malformation resulting in hypoplasia or atrcsia of the left ventricle,ascending aorta,aortic and mitral valves. Diminished flow through the ...
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Hypoplastic left heart syndrome(HLHS) is a serious congenital cardiovascular malformation resulting in hypoplasia or atrcsia of the left ventricle,ascending aorta,aortic and mitral valves. Diminished flow through the left side of the heart is clearly a key contributor to the condition,but
The main purpose of this study was to investigate the effect of Ca/calmodulin（CAM）on voltage-gated sodium channels（VGSCs） in the hippoeampal neuronal culture model of epileptiform *** data showed that the channel ...
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The main purpose of this study was to investigate the effect of Ca/calmodulin（CAM）on voltage-gated sodium channels（VGSCs） in the hippoeampal neuronal culture model of epileptiform *** data showed that the channel activity of persistent,non-inactivating Na current(I)
目的:脂肪组织在机体血糖稳态的调节中发挥重要作用,其慢性炎症是胰岛素抵抗和2型糖尿病发生发展的的主要因素之一。降钙素基因相关肽超家族:如降钙素基因相关肽,肾上腺髓质素,能改善机体的炎症反应。新近发现的降钙素基因相关肽超家族成员垂体中叶素(Intermedin),是一种重要的心血管保护肽。我们的研究是探讨IMD在高脂饮食诱导的脂肪组织炎症和机体胰岛素抵抗中的作用。材料和方法:16周的C57小鼠高脂饮食喂养8周,在喂养的第5周,皮下植入含IMD的微量渗透泵(300ng/kg/hour),对照组为生理盐水组。高脂饮食喂养8周后,我们检测了胰岛素抵抗相关指标及炎症指标。离体实验中,我们主要使用大鼠原代脂肪细胞和分化的3T3-L1脂肪细胞。结果:在高脂饮食喂养小鼠及db/db小鼠中,脂肪组织IMD和受体激活调节蛋白1、2、3(RAMP1,RAMP2,RAMP3)表达均下降。在高脂饮食喂养8周后,通过检测空腹血糖、血胰岛素、糖耐量试验(GTT)、胰岛素耐量试验(ITT)及正糖高胰岛素钳夹试验(EHC)等发现,高脂饮食第四周皮下持续微量给与IMD组小鼠胰岛素敏感性相比对照组明显改善。同时IMD处理组小鼠脂肪组织胰岛素信号水平明显增强,而血浆炎症因子(TNFα、IL-6、MCP-1等)和游离脂肪酸水平显著下降。脂肪组织H&E及免疫学染色显示IMD处理组小鼠,脂肪细胞脂滴更小,更少的"花环状结构"(crown like structures)及炎症细胞浸润,实时定量PCR和免疫组化显示IMD组炎症因子表达水平下降。为了探讨IMD改善高脂饮食小鼠胰岛素抵抗和脂肪组织炎症的机制,我们培养了大鼠原代脂肪细胞和分化的3T3-L1脂肪细胞,IMD预处理脂肪细胞,能改善由TNFα引起的炎症因子mRNA表达和蛋白分泌。抑制TNFα引起的P38和JNK磷酸化,以及IκBα的降解和NF-κb转录活性。同时我们发现IMD能时间和剂量依赖性的激活AMPK,ACC,使用.AMPK抑制剂Compound C能抑制IMD对NF-κb的作用。结论:上述结果显示IMD处理能改善高脂饮食引起的小鼠胰岛素抵抗,其可能的机制是IMD通过AMPK-NF-κB通路抑制脂肪组织的慢性炎症。
Objectives:Hindlimb unweighted rats are commonly used to simulate the hemodynamic variation of human in microgravity and could induce functional remodeling of arteries,the mechanisms of which have not yet been fully *...
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Objectives:Hindlimb unweighted rats are commonly used to simulate the hemodynamic variation of human in microgravity and could induce functional remodeling of arteries,the mechanisms of which have not yet been fully *** are small invaginations on cell membrane enriched with cholesterol and sphingolipids which could be modified by mechanical stresses
Studies have shown that low doses of bradykinin(BK) can induce vascular relaxation and high dose of BK can induce vascular *** the mechanisms are not *** compared the role and mechanism of high doses of BK-induced vas...
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Studies have shown that low doses of bradykinin(BK) can induce vascular relaxation and high dose of BK can induce vascular *** the mechanisms are not *** compared the role and mechanism of high doses of BK-induced vascular contraction in normal and shock
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