Background:Neuropathic pain (NP) continues to be challenging to treat due to lack of effective *** astrocytes play a pivotal role in nerve injury-induced central *** studies indicated thatglia-mediated inflammatory re...
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Background:Neuropathic pain (NP) continues to be challenging to treat due to lack of effective *** astrocytes play a pivotal role in nerve injury-induced central *** studies indicated thatglia-mediated inflammatory reactions contribute to the introduction and development of neuropathic ***,the activation of the extracellular regulated protein kinases (ERK)/tumor necrosis α pathway in astrocytes has been reported to be critical for spinal astrocytic activation and neuropathic pain (NP) development after spinal nerve ligation (SNL).8-O-acetyl-SM (8-OaS), a major active component of a traditional Chinese drug, Lamiophlomisrotata, possesses potent immuno-suppressive ***:The effects of lidocaine, ketamineand 8-OaS on SNL-induced mechanical allodynia were investigated using behavioral *** activation of spinal glial fibrillary acidic protein (GFAP) , pERKand TNF-αwas observed by immunofluorescent histochemistry and Western ***:In the present study, we observed that intrathecalinjection of lidocaine,ketamineand8-OaS significantly reduced SNL-induced mechanical hypersensitivity and the anti-nociception effect of 8-OaS in a dose dependent *** immunostaining showed that ERK was highly expressed in astrocytes after SNL,indicatingthat ERK signaling pathway participates in the activation of *** further observed that intrathecalinjection of ERK inhibitor (PD98059)significantly decreased spinal astrocytic activation and TNF-α ***, Western blot analysis revealed that intrathecal 8-OaS (ED50) could also reduce SNL-induced ERK phosphorylation and TNF-α ***, no significant increasesin mechanical hindpawwithdrawal threshold and TNF-αtexpression were observed compared with the 8-OaS-treated groupafter co-treatment with 8-OaS and ***: our results suggest thatthe analgesic effects of 8-OaS in NP are mainly mediatedby the down-regulation of SNL-induced astr
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