Objective To explore the effective ingredients of traditional Chinese medicine compound in different aperture dynamic migratbn patterns in the process of ceramic membrane micro filtration,compare it's micro filtra...
详细信息
Objective To explore the effective ingredients of traditional Chinese medicine compound in different aperture dynamic migratbn patterns in the process of ceramic membrane micro filtration,compare it's micro filtration flux and solid inclusion removal rate by taking Compound Banlangen Liyan Granules（Radix Isatidis,Radix Scrophulariae,Radix Platycodi,Radix Glycyrrhizae,etc.） water extraction liquid as the research objectMethod Continuous sampling of many times in the process of ceramic membrane micro filtration,HPLC method was used for determinating the contents of 6 effective components(adenosine,（R,S）-goitrin,liquiritin,harpagosid,platycodin-D and monoammonium glycyrrhizinate) in compound at the same time,and the experiment studied the dynamic migration *** For the Compound Banlangen Liyan Granules water extract,in the process of 200-nm ceramic membrane microfiltration,the dynamic migration rates of the 6 effective components ranged between 71%~ 104%,the average migration rate was 85%,the microfiltration flux attenuation was smaller,the stable flux was in 426 ~ 340 L·m-2·h-l,solid inclusion removal rate was 21.0%.But in 50-nm ceramic membrane,the dynamic migration rates of the 6 effective components ranged between 83%~ 107%,the average migration rate was 83%,the micro filtration flux decline was larger,the stable flux was in 258 ~ 228 L·m-2·h-1,solid inclusion removal rate was 23.9%.Conclusion The experiment preliminary reflected the live migration process of the traditional Chinese medicine compound effective ingredients in two different aperture dynamic ceramic membrane micro filtrationlt layed a effective material migration theoretical foundation for popularization and application of modern ceramic membrane technology in traditional Chinese medicine refining.
Objective:In this research,a novel vinoreIbine-loaded aptamer（Apt） conjugated lipid-polymer hybrid nanoparticles（Apt-NP/VRL） were constructed for specific targeting MUC1 protein over expressed tumor ***:Apt-NP/VRL...
详细信息
Objective:In this research,a novel vinoreIbine-loaded aptamer（Apt） conjugated lipid-polymer hybrid nanoparticles（Apt-NP/VRL） were constructed for specific targeting MUC1 protein over expressed tumor ***:Apt-NP/VRL were prepared by self-assembling method using lecithin,DSPE-PEG2000-COOH,and poly（lactic-co-glycolic acid）(PLGA) as carrier *** formulation was optimized by orthogonal *** aptamer was conjugated to DSPE-PEG2000-COOH through EDC and NHS coupling（Figure 1）.Though changing the ratio of DSPE-PEG2ooo-Apt,NPs with different aptamer densities were synthesized and ***-6 labeled nanoparticles（Apt-NP/Cou-6） were prepared to evaluate the cellular uptake *** anti-tumor activity of Apt-NP/VRL to MCF-7 cell and HpG2 cell was ***:TEM indicated Apt-NP/VRL were about 128.1-166.5 nm,with a core-shell construction and the shell was 10 nm（Figure 2A）.XPS showed Apt was successfully conjugated to the surface of NP/VRL（Figure 2B）.The encapsulation efficiency was50.42-57.88%.The MCF-7 cell uptake of Apto.5-NP/VRL,Apti-NP/VRL,Apt-NP/VRL,Apt-NP/VRL,Apt-NP/VRL were increased with the increase of aptamer density（Figure 3）,and Figure 4 showed that the uptake of Apt-NP/VRL has been enhanced compared with Apto-NP/VRL in MCF-7 cells,indicating increased aptamer density improved targeting efficiency（p<0.05）.By increasing aptamer density,there was a increase in the inhibitory of MCF cell by Apto.5-NP/VRL,Apt-NP/VRL,Apt-NP/VRL,Apt-NP/VRL,Apt-NP/VRL compared with HepG2 cell（p<0.05）(Figure 5).All the results indicating that conjugate of aptamer has enhanced cellular uptake and toxicity of Apt-NP/VRL to MUC1 cell（p<0.05）.Conclusion:Aptamer conjugated lipid-polymer hybrid nanoparticles has been fabricated for MUC1 over expressed cancer cell targeting and ***-NP/VRL can delivery drugs specifically to MUC1 over expressed cancer cells which enhanced cell uptake and *** increasing the aptamer density on the su
暂无评论