Objective To study the advantage of SVF cells as a kind of ideal tissue engineering seed *** After isolating(0.125% collagenase type Ⅰ), SVF cells was labeled with DiI in *** was induced to adipogenic, osteogenic and...
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Objective To study the advantage of SVF cells as a kind of ideal tissue engineering seed *** After isolating(0.125% collagenase type Ⅰ), SVF cells was labeled with DiI in *** was induced to adipogenic, osteogenic and chondrogenic differentiation with the corresponding inductive culture *** was stained by oil O, alizarin red and alcian blue *** cells was co-combined with type Ⅰ collagen *** adhesion ratio of the SVF was determined by cell count *** morphological features and function of the SVF were observed by invert optical microscope and scanning electron *** we could get a lot of SVF cells quickly with 0.125% type Ⅰ *** is a kind of ideal fluorsecent dye for *** cells could be induced to adipogenic, osteogenic and chondrogenic differentiation with the contesponding inductive culture *** cells were able to attach, grow and proliferate well on the *** rate of SVF was (92.596±2.268)%, while that of DII labeling group was (92.488±2.262)% (P =0.964).Conclusion SVF cells could be obtained quickly with 0.125% type Ⅰ *** could be induced to adipogenic, osteogenic and chondrogenic *** Ⅰ scaffold exhibits excellent cellular compatibility to SVF *** cells is a kind of ideal seed cells which has a bright future in clinical practice.
Background: Inadequate projection of the midface skeleton results in midface *** with this skeletal morphology tend to have prominent eyes and *** of skeletal support for the midface soft tissue envelope predisposes t...
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Background: Inadequate projection of the midface skeleton results in midface *** with this skeletal morphology tend to have prominent eyes and *** of skeletal support for the midface soft tissue envelope predisposes to premature cheek descent, resulting in palpebral fissure distortion and lower lid "bags," an appearance of early *** midface concavity often is seen with the craniofacial dysostoses of maxillary *** the past, surgeons have had 2 techniques with which to improve facial bony contour: osteotomy with mobilization of facial bony segments and placement of implants (foreign-body materials or autologous cartilage or bone) over the bone, under the soft *** this article, we discuss a relatively new third alternative, the use of *** have found that the use of lipostructure can improve the concave face (maxillary hypoplasia).In fact ,autologous fat grafts have gained popularity among Cranio-Maxillofacial surgeons within the past ***: We describe the technique for midfacial contour augmentation using lipostructure and present the results of 158 cases (of 325cases with lipostructure)treated between 2010 and ***: We conducted a preoperative evaluation to determine for which patients the procedure was appropriate and to identify the need for additional *** was harvested with the use of 10-mL syringes with 2 mm *** 5 mL of fat was harvested in each *** the donated fat was centrifuged and purified, the purified fat was infiltrated by means of a percutaneous approach with 18-or 20-gauge needles to redluce trauma and avoid *** (2-mm-wide) rolls of fat grafts were placed in well-vascularized tissues to prevent *** amount of fat infiltrated depended on the flatness of the area and accord with aesthetic ***: Most (more than 90%) patients had saisfactory outcomes by a single session after follow-up ranging from 3 months to 3 ***
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