目的 使用主要代表性过敏原诊断的方式排除因交叉反应及其他各种原因对结果的干扰,建立更简便快捷更精确的诊断方法,以便进行更有针对性更有效的治疗。方法 使用粉尘螨主要代表性过敏原Der f 1,Der f 2以及泛过敏原/交叉过敏反应组分tro...
详细信息
目的 使用主要代表性过敏原诊断的方式排除因交叉反应及其他各种原因对结果的干扰,建立更简便快捷更精确的诊断方法,以便进行更有针对性更有效的治疗。方法 使用粉尘螨主要代表性过敏原Der f 1,Der f 2以及泛过敏原/交叉过敏反应组分tropomyosin包板,依据UniCAP诊断的血清过敏级别及检测诸多影响参数来设计正交试验,并使不同级别的血清在96孔板上能均匀分布,设置阴性和阳性对照,依据ELISA读板的吸光值来判断病人血清阳性与否,并进行相应分级,使用统计学方法比较传统诊断方法与主泛过敏原联合诊断方法的准确性、灵敏度、特异性及MCC值。同时结合临床脱敏治疗病人随访,分析疗效与主-泛过敏原阳性与否的对应关系。结果 使用ELISA进行组分诊断的结果与常规诊断存在一致性,但有部分病人对尘螨主要过敏原并不显示阳性;而部分病人对交叉反应过敏原反应阳性且对尘螨主要过敏组分反应性较低,这一部分病人的脱敏治疗疗效欠佳。结论 主要代表性过敏原与泛过敏原联合诊断可以反映传统诊断方法的结果,同时能鉴别并排除传统方法中因交叉反应及其他因素的干扰,提高诊断准确率,为后续开展准确而高效的治疗提供重要依据。
The aim of this study was to assess the diagnostic value of epidermal growth factor (EGF),CA125,and CA153 in BALF of lung cancer.79 patients with suspected lung cancer were *** the patients undergo fiberscopic examina...
The aim of this study was to assess the diagnostic value of epidermal growth factor (EGF),CA125,and CA153 in BALF of lung cancer.79 patients with suspected lung cancer were *** the patients undergo fiberscopic examination and bronchoalveolar lavage fluid (BALF) samples were collected during the *** of EGF,CA125,and CA153 in BALF were determined by commercially available test kits.
目的 Pulmonary alveolar proteinosis(PAP) is a rare pulmonary disease. There are three clinically distinct forms of PAP: congenital, secondary, and *** PAP is associated with cancers, mostly with hematologic malignanc...
详细信息
目的 Pulmonary alveolar proteinosis(PAP) is a rare pulmonary disease. There are three clinically distinct forms of PAP: congenital, secondary, and *** PAP is associated with cancers, mostly with hematologic malignancies.方法 We report the first case of PAP that occured 3 months before a diagnosis of chronic myelomonocytic leukemia(CMML) and review advances in treatment of SPAP. 结果 A 66-year-old Chinese man presented with recurrent coughand progressive dyspnea was admitted to our *** chest computed tomographyscan disclosed bilateral ground-glass opacities with interlobular septal thickening in lower *** lung biopsy(TBLB) revealed PAS-positive panlobular diffuse areas of intra-alveolar granular material consistent with *** studies revealed peripheral blood monocytes>3.6×103cells/uL and bone marrow aspirates showed monocytes 30%.He was dignosed with CMML and treated with γ-interferon and *** whole lung lavage, his symptoms relieved gradually and the radiological manifestation absorbed 3 months later. 结论 So for PAP patients, underlying disease including hematologic disorders should be considered.
目的 In the previous studies,we observed a close association between asthma and a distinctive palm dermatoglyphic pattern,but the underlying mechanisms have not been *** explore the genetic mechanisms of the distincti...
详细信息
目的 In the previous studies,we observed a close association between asthma and a distinctive palm dermatoglyphic pattern,but the underlying mechanisms have not been *** explore the genetic mechanisms of the distinctive palm dermatoglyphic pattern and asthma.方法 A total of 400 patients with allergic asthma and 200 normal subjects were *** was extracted from blood cells and 7 IL-4/IL-13 SNPs related with asthma were analyzed by PCR-RFLP method.结果 Three IL-4/IL-13 SNPs,rs2158177(Recessive model,p=0.021),rs1800925(Recessive model,p=0.029),rs2243283(Recessive model:p=0.049)were involved in the association with *** one SNP,rs1295683,is associated with the positive palm pattern in Recessive model(P=0.048).No same SNP was related with both asthma and the special palm pattern.结论 IL-4/IL-13 polymorphisms could be genetic basic for asthma or the typical palm,but more investigations are needed to identify the connection of asthma and palm dermatoglyphic pattern and its genetic basic.
目的 Lipopolysaccharide(LPS)and endothelin-1(ET-1)are critical pathogenic factors in sepsis-induced pulmonary hypertension,we hypothesized that they have a coordinated action in the pathogenesis of this disease.方法 V...
详细信息
目的 Lipopolysaccharide(LPS)and endothelin-1(ET-1)are critical pathogenic factors in sepsis-induced pulmonary hypertension,we hypothesized that they have a coordinated action in the pathogenesis of this disease.方法 Vasoconstrictor-induced cell contraction and global intracellular Ca2+([Ca2+]i)mobilization in the absence or presence ofLPS were determined in Freshly isolated rat *** effect of LPS on endothelin-1(ET-1)induced store-operated calciumentry(SOCE)was also *** receptor potential canonical(TRPC)channels,the molecular basis of SOC channels,were evaluated by real-time PCR for their mRNA *** direct effect of LPS on TRPC channels was assessed in TRPC3-6over-expressed HEK293 cells using whole-cell patch clamp.
暂无评论