目的 To observe the endocannabinoid system(ECs)in cannabinoid receptor 1(CB1R)situation and trend after the intervention CB1R expressed in bone tissue,and by measuring the levels of bone-derived enzyme *** After using...
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目的 To observe the endocannabinoid system(ECs)in cannabinoid receptor 1(CB1R)situation and trend after the intervention CB1R expressed in bone tissue,and by measuring the levels of bone-derived enzyme *** After using CB1R antagonist rimonabant intervention can alleviate bone metabolism and risk of abnormal bone structure in rats CIH.方法 Select 48 male SD rats,by establish the OSAS typical pathological model-chronic intermittent hypoxia(CIH)experimental animal models,according to the different methods of exposure and cycle times were randomly divided into six groups,namely 4,6W hypoxic group(IH),4,6W the control group(ON),4,6W hypoxic intervention group(HI),where the HI group were given intraperitoneal injection CB1R before modeling antagonist 1.5mg/kg/d were hypoxia *** the experiment of 4W,6W randomly selected half of the rats when the experiment was terminated,taken femoral vein 5ml,1h standing later,centrifuged the supernatant to determine the serum TRAP content by using ELASA method;then intercept the bone tissue of rats in each group with decalcification solution soak until softened tissue,after paraffin embedding machine slicer cut in a uniform thickness of bone tissue placed on a glass slide,HE staining in light microscopy whether the change in bone microarchitecture;observe the expression of bone tissue CB1R using immunohistochemistry and determination.结果 *** determination：After modeling,4W and 6W-IH group were higher than TRAP modeling before 4,6W-ON group,there was difference(t =-7.930,P ＜0.001),and Comparison between ON TRAP activity level group,the difference was not statistically significant(t =-1.273,P = 0.224);after the intervention,4W and 6W-HI group TRAP levels were lower than before the intervention 4,6W-IH group,there are statistical difference(t =-7.930,P ＜0.001),these results prompted increased after hypoxia time TRAP levels can be elevated above normal,but after the intervention,TRAP levels can interfere with the extension
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