Chemoresistance hinders the curative cancer ***(miRNAs)have been identified as key players in the chemoresistance of osteosarcoma,which is the most common primary malignancy of *** sequencing based miR-omic and quanti...
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Chemoresistance hinders the curative cancer ***(miRNAs)have been identified as key players in the chemoresistance of osteosarcoma,which is the most common primary malignancy of *** sequencing based miR-omic and quantitative real-time PCR analyses suggested that miR-193a-3p expression is down-regulated by DNA methylation at their promoter region in multi-chemoresistant cell line(SJSA-1)relative to multi-chemosensitive cell line(G-292).Introduction of miR-193a-3p mimic into SJSA-1 or antagomiR into G-292 confirmed their roles in osteosarcoma ***,bio-informatic prediction along with biochemical assays clearly suggested that the IRS2 gene is the target of *** target indeed involved in the miR-193a-3p-induced suppression of osteosarcoma cell *** together,our data established that miR-193a-3p plays important roles in osteosarcoma multi-chemoresistance through down-regulation of the IRS2 gene,which might serve as useful biomarkers of osteosarcoma diagnosis and potential candidates for the treatment of osteosarcoma.
Objective: Systemic inflammation contributes to cardiovascular disease in patients with type 2 diabetes,and elevated white blood cell(WBC)counts are an established risk *** goal is to describe changes in WBCs and infl...
Objective: Systemic inflammation contributes to cardiovascular disease in patients with type 2 diabetes,and elevated white blood cell(WBC)counts are an established risk *** goal is to describe changes in WBCs and inflammatory markers after glycemic reductions in ***: This study enrolled 63 subjects with poorly controlled diabetes,defined as hemoglobin A1c(HbA1c)≥8%[64 mmol/mol].Circulating granulocytes and mononuclear cells were separated by histopaque double-density *** markers from these isolated WBCs were assessed at baseline and after 3 months of medical ***: After 3 months,significant glycemic reduction,defined as a decrease in HbA1c≥1.5%,occurred in 42 *** plasma glucose decreased by 47%(165.6 mg/dL),and HbA1c decreased from 10.2±1.8 to 6.8±*** reductions were associated with a 9.4%decrease in total WBC counts,10.96%decrease in neutrophils,and 21.74%decrease in *** mRNA levels of inflammatory markers from granulocytes and mononuclear cells decreased,including receptor for advanced glycation endproducts;S100 calcium binding proteins A8,A9,A12;krüppellike factor 5;and ***,circulating levels of IL-1β and C-reactive protein *** dose was a mediator between HbA1c and both total WBC and neutrophil counts,but not changes in WBC inflammatory *** contrast,the 17 subjects without significant glycemic reductions showed no significant differences in their WBC counts and proteins of inflammatory ***: Significant glycemic reduction in subjects with poorly controlled diabetes led to reduced circulating WBC counts and inflammatory gene expression.
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