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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是91-100 订阅

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A compact polarization converter to observe molecular orientation

A compact polarization converter to observe molecular orient...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Hashimoto, Mamoru Yoshiki, Keisuke Kanamaru, Ryosuke Hashimoto, Nobuyuki Araki, Tsutomu Osaka Univ Grad Sch Engn Sci 1-3 Machikaneyama Toyonaka Osaka 5608531 Japan Citizen Watch Co Ltd MHT R&D Dept Tokorozawa Saitama 3598511 Japan

ISBN: (纸本)9780819465566

We developed a compact polarization-converter using two liquid-crystal spatial-light-modulators with eight electrodes. The converter converted a linearly polarized beam to two orthogonal linearly polarized beams and a radially polarized beam, and the direction of the electric filed at the focal point were controlled three-dimensionally. We constructed a second-harmonic-generation microscope using the polarization-converter to observe three-dimensional molecular orientation and demonstrated the detectability of molecular orientation.

关键词： molecular orientation nonlinear microscopy second-harmonics-generation pupil filtering radially polarized beam

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Multiple beam interference confocal microscopy - a tool for morphological investigation of living cells and tissues

Multiple beam interference confocal microscopy - a tool for ...

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Joshi, NV Medina, H Univ Los Andes Fac Med Dept Physiol Merida 5101 Venezuela

ISBN: (纸本)081943535X

Multiple beam interference system is used in conjunction with a conventional scanning confocal microscope to examine the morphology and construction of 3-D images of Histolytic Ameba and parasite Candida Albicans. The present combination permits to adjoin advantages of both systems, namely the vertical high contrast and optical sectioning. The interference pattern obtained from a multiple internal reflection of a sample, sandwiched between the glass plate and the cover plate, was focussed on an objective of a scanning confocal microscope. According to optical path differences, morphological details were revealed. The combined features, namely improved resolution in z axis, originated from the interference pattern and the optical sectioning of the confocal scanning system, enhance the resolution and contrast dramatically. These features permitted to obtain unprecedented images of Histolytic Ameba and parasite Candida Albicans. Because of the improved contrast, several details like double wall structure of candida, internal structure of ameba are clearly visible.

关键词： Confocal microscopy Microscopes Digital image correlation Glasses Tissues Refractive index 3D image processing microscopy Optical scanning systems Phase contrast

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Computational super-resolution microscopy: leveraging noise model, regularization and sparsity to achieve highest resolution 27

Computational super-resolution microscopy: leveraging noise ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Xing, Jian Chen, Simeng Becker, Stephen Yu, Jiun-Yann Cogswell, Carol Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA Univ Colorado Dept Appl Math Boulder CO 80309 USA

ISBN: (纸本)9781510632547

We report progress in algorithm development for a computation-based super-resolution microscopy technique. Building upon previous results, we examine our recently implemented microscope system and construct alternative processing algorithms. Based on numerical simulations results, we evaluate the performance of each algorithm and determine the one most suitable for our super-resolution microscope.

关键词： Super-resolution microscopy regularization computational microscopy

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Correction accuracy in image-based adaptive optics for nonlinear microscopy

Correction accuracy in image-based adaptive optics for nonli...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Facomprez, Aurelie Beaurepaire, Emmanuel Debarre, Delphine Ecole Polytech ParisTech Lab Opt & Biosci CNRS F-91128 Palaiseau France INSERM F-91128 Palaiseau France

ISBN: (纸本)9780819488701

We investigate the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. In this paper we focus on the case of two-photon excited fluorescence. Using analytical, numerical and experimental results, we show that using a suitable number of measurements, accurate correction can be achieved for up to 2 rad rms initial aberrations even without optimisation of the correction modes. We demonstrate that this correction can be achieved using low light levels to minimise photobleaching and toxicity, and we provide examples of such optimised correction.

关键词： Adaptive optics aberration correction multiphoton microscopy nonlinear microscopy

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Single image sectioning using spatial frequency domain imaging 24

Single image sectioning using spatial frequency domain imagi...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIV

作者： Hoffman, Zachary R. DiMarzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Draper Lab 555 Technol Sq Cambridge MA 02139 USA

ISBN: (数字)9781510605824

ISBN: (纸本)9781510605817;9781510605824

Structured illumination microscopy (SIM) works well to produce depth information by removing undesired light from out of focus planes within a specimen. However, it generally requires multiple images in order to produce sectioning. We show that, under the right conditions, we can leverage Spatial Frequency Domain Imaging (SFDI) to produce both sectioning and relative depth information about a specimen using only a single image. After processing, we find that the axial resolution is comparable to that of traditional 3 phase SIM. With this technique, we are able to produce real-time videos in-vivo at 21 frames per second. Additionally, no moving parts are needed to produce sectioning at depth.

关键词： Structured Illumination optical sectioning super-resolution incoherent SFDI

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3D imaging of the cleared intact murine colon with light sheet microscopy 13

3D imaging of the cleared intact murine colon with light she...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Zufiria, B. Bocancea, D. I. Gomez-Gaviro, M. V. Vaquero, J. J. Desco, M. Fresno, M. Ripoll, J. Arranz, A. Univ Autonoma Madrid CSIC Ctr Mol Biol Severo Ochoa CBMSO Madrid Spain Univ Carlos III Madrid Dept Bioengn & Aerosp Engn Madrid 28911 Spain Inst Invest Sanitaria Gregorio Maranon Madrid 28007 Spain

ISBN: (纸本)9781628419474

We here show 3D light sheet microscopy images of fixed and cleared murine colon tissue in-toto, which offer relevant cellular information without the need for physically sectioning the tissue. We have applied the recently developed CUBIC protocol (Susaki et al. Cell 157: 726, 2014) for colon tissues and have found that this clearing protocol enables imaging all the way to the central part of the lumen with cellular resolution, thus opening new ways for 3D imaging of colon samples

关键词： light sheet microscopy CUBIC protocol tissue clearing

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acquisition of multiple image stacks with a confocal laser scanning microscope

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Zuschratter, W Steffen, T Braun, K Herzog, A Michaelis, B Scheich, H Fed Inst Neurobiol D-39118 Magdeburg Germany

ISBN: (纸本)0819427004

image acquisition at high magnification is inevitably correlated with a limited view over the entire tissue section. To overcome this limitation we designed software for multiple image-stack acquisition (3D-MISA) in confocal laser scanning microscopy (CLSM). The system consists of a 4 channel Leica CLSM equipped with a high resolution z-scanning stage (Leica Lasertechnik) mounted on a xy-motorized stage (Marzhauser). The 3D-MISA software is implemented into the microscope scanning software (Scanware, Leica Lasertechnik) and uses the microscope settings (magnification, zoom) for the movements of the xy-stage. It allows storage and recall of 70 xyz-positions and the automatic 3D-scanning of image arrays between selected xyz-coordinates. The number of images within one array is limited only by the amount of disk space or memory available. Although for most applications the accuracy of the xy-scanning stage is sufficient for a precise alignment of tiled views, the software provides the possibility of an adjustable overlap between two image stacks by shifting the moving steps of the xy-scanning stage. After scanning a tiled image gallery of the extended focus-images of each channel will be displayed on a graphic monitor. In addition, a tiled image gallery of individual focal planes can be created. In summary, the 3D-MISA allows 3D-image acquisition of coherent regions in combination with high resolution of single images.

关键词： multi-image-stacks confocal laserscanning microscopy 3D image acquisition

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Confocal microscopy with variable-focal length microlenses

Confocal microscopy with variable-focal length microlenses

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Mac Raighne, A Yang, LS Dunbar, LA McCabe, EM Scharf, T Trinity Coll Dublin Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819452327

Confocal microscopy has a unique optical sectioning property, which allows three-dimensional images at different depths. Use of a microlens array is a potential alternative to the Nipkow disk for parallel imaging with high throughput in real-time confocal microscopy. The use of variable-focal-length microlenses can provide a way to axially scan the foci electronically avoiding the inflexible mechanical movement of the lens or the sample. Here we demonstrate a combination of a variable-focal-length microlens array and a fibre optic bundle as a way to create a high throughput aperture array that would be potentially applied as confocal imaging in vivo biological specimens. The incident side of the microlens array was determined by examining the focus distribution in the axial direction. The variation of the focal length obtained by changing the voltage and corresponding focus intensity were measured through a conventional microscope. Meanwhile, the fibre bundle was characterized by coupling with either coherent or incoherent light source. We use the fibre bundle as both a multiple aperture and an image-carrying element and combine it with a microlens array to built up a confocal system.

关键词： confocal microscopy endoscopy microlenses fibre bundle

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Autostereoscopic visualization of 3D time-varying complex objects in volumetric image sequences

Autostereoscopic visualization of 3D time-varying complex ob...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Benassarou, A. Valette, G. Debons, D. Remion, Y. Lucas, L. CReSTIC SIC Rue CrayeresBP 1035 F-51687 Reims 2 France 3DTV Solut F-51100 Reims France

ISBN: (纸本)9780819484413

This paper presents 4dVizMed, a framework for interactive analysis and autostereoscopic visualization of 3d time-varying objects in volumetric image sequences. It combines a deformable surface model which automatically tracks volumetric features, real-time multi-view stereo volume rendering, and some interactive tools for manipulation and quantization. Our method is based on a topological feature tracking process, using a flow-based paradigm and a deformable surface model. It tracks through time the evolution of the components of an isosurface and their interaction with other components. We focus on the difficulties of visualizing 4d volume data, and we report the results of preliminary experiments designed to evaluate the utility of autostereoscopic displays for this purpose.

关键词： Visualization 3D modeling 3D image processing Autostereoscopic displays image visualization image segmentation 3D displays 3D visualizations image processing Volume rendering

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Adaptive Optics for Wide-field microscopy

Adaptive Optics for Wide-field Microscopy

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Bourgenot, C. Saunter, C. D. Girkin, J. M. Love, G. D. Univ Durham Dept Phys Ctr Adv Instrumentat Durham DH1 3LE England

ISBN: (纸本)9780819484413

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensorless AO). In the latter system the determination of the best mirror shape is found via two routes. In the first an optimization algorithm using a Simplex search pattern is used with an initial random set of mirror shapes. We then explore the use of specific Zernike terms as our starting basis set. In both cases the final optimization performance is not affected by the choice of optimization metric. We then describe an open loop AO system in which the equivalent of a laser guide star is used as the light source for the wavefront sensor.

关键词： Optical microscopy Aberration correction Adaptive Optics

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