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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是91-100 订阅

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Simultaneous quantitative depth mapping and extended depth of field for 4D microscopy through PSF engineering

Simultaneous quantitative depth mapping and extended depth o...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Zahreddine, Ramzi N. Cormack, Robert H. Cogswell, Carol J. Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA

ISBN: (纸本)9780819488701

An extended depth of field (EDF) microscope that allows for quantitative axial positioning has been constructed. Past work has shown that EDF microscopy allows for features in varying planes to appear sharply focused simultaneously, however an inherent consequence of this is that depth information is lost. Here, a specifically engineered phase plate is used to create a point spread function (PSF) that contains both of the necessary attributes for extended depth of field and quantitative depth mapping. A two-camera solution is used to separate and capture the information for individualized post processing. The result is a microscope that can serve as an essential tool for full 3D, real-time imaging.

关键词： 4D microscopy PSF engineering extended depth of field quantitative depth ranging

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Optical coherence computed tomography for quantitative 3D imaging of adult zebrafish (Withdrawal Notice) 25

Optical coherence computed tomography for quantitative 3D im...

引用

Three-dimensional and multidimensional microscopy: image acquisition and processing XXV 2018

作者： Van Der Horst, Jelle Trull, Anna Kalkman, Jeroen Technische Univ. Delft Netherlands

“Publisher’s Note: This conference presentation, originally published on 3/21/18, was withdrawn per author request.”

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614833

“Publisher’s Note: This conference presentation, originally published on 3/21/18, was withdrawn per author request.”

关键词： Tissue optics Coherence (optics) Computed tomography Optical coherence tomography Optical imaging Refractive index Signal attenuation 3D image processing Light scattering Optical tomography

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Three-dimensional data acquisition with aberrations correction for video-rate microscopy

Three-dimensional data acquisition with aberrations correcti...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Samim, Masood Cisek, Richard Sandkuijl, Daaf Tretyakov, Ian Siu, Sam Musikhin, Sergey Barzda, Virginijus Univ Toronto Dept Chem & Phys Sci Mississauga ON L5L 1C6 Canada

ISBN: (纸本)9780819484413

We demonstrate a multimodal, multifocal, differential nonlinear optical microscope, which is equipped with a pair of deformable mirrors and a Shack-Hartmann sensor for dynamic wavefront manipulation. The optical wavefronts of a home built Yb:KGW femtosecond (1028 nm) laser-beams are engineered to perform multidepth focusing in differential mode with simultaneous corrections for optical aberrations. The 39-actuator deformable mirrors provide fast reshaping of the wavefront and optical aberrations correction of the diffraction-limited focal volume allowing for fast axial scanning. Combination of similar to 200 frames per second lateral scanning with fast refocusing enables a three-dimensional video rate scanning capability, which is essential for studying rapid dynamics in biological organisms, such as blood flow, cardiac contractions, and motility of microorganisms in a three-dimensional volume.

关键词： Deformable mirrors adaptive optics nonlinear optical microscopy harmonic generation microscopy non-invasive in vivo imaging three-dimensional imaging multi-contrast microscopy

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Spectral-domain interferometry for quantitative DIC microscopy

Spectral-domain interferometry for quantitative DIC microsco...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Li, Chengshuai Zhu, Yizheng Virginia Tech Bradley Dept Elect & Comp Engn Blacksburg VA 24060 USA

ISBN: (纸本)9780819498625

A spectral-domain differential interference contrast (SD-DIC) microscopy system is presented for quantitative imaging of both reflective and transparent samples. The spectral-domain interferometry, combined with the common-path DIC geometry, provides a shot noise-limited sensitivity of 14.3pm in optical pathlength gradient measurement. The optical resolution of the system was characterized using images of a USAF resolution target. Fused silica microspheres were imaged to demonstrate the reconstruction of two-dimensional optical pathlength topography from measured gradient fields. The exquisite sensitivity of the system showed potential in quantitative imaging of sub-diffraction limit objects such as gold nanoparticles.

关键词： Differential interference contrast quantitative phase imaging spectral-domain interferometry

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Computational super-resolution microscopy: leveraging noise model, regularization and sparsity to achieve highest resolution 27

Computational super-resolution microscopy: leveraging noise ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Xing, Jian Chen, Simeng Becker, Stephen Yu, Jiun-Yann Cogswell, Carol Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA Univ Colorado Dept Appl Math Boulder CO 80309 USA

ISBN: (纸本)9781510632547

We report progress in algorithm development for a computation-based super-resolution microscopy technique. Building upon previous results, we examine our recently implemented microscope system and construct alternative processing algorithms. Based on numerical simulations results, we evaluate the performance of each algorithm and determine the one most suitable for our super-resolution microscope.

关键词： Super-resolution microscopy regularization computational microscopy

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Design and performance evaluation of reflection confocal microscopy using acousto-optical deflector and slit detector

Design and performance evaluation of reflection confocal mic...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Lee, SW Kang, DK Yoo, HK Gweon, DG Lee, SW Kim, KS Korea Adv Inst Sci & Technol Dept Mech Engn Taejon South Korea

ISBN: (纸本)0819452327

We describe the design and the implementation of reflection confocal scanning microscopy (CSM) using an acousto-optical deflector (AOD) for the fast horizontal scan and a galvanometer mirror (GM) for the slow vertical scan. In the beam scanning system it is important to maintain the lateral and the axial performance during scanning operation. We propose a simple method to design a scanning system using the finite ray tracing and the diffraction theory. We define a cost function which contains the effect of aberrations on the performance of microscopy. We construct the designed system and evaluate its performance. The OSLO simulation shows that the performances of CSM are not changed with deflection angle. So we conclude that the beam scanning system is properly designed. In addition, we propose an image formation method and show images obtained with the system.

关键词： confocal microscopy beam scanning system acousto-optical deflector slit detector finite ray tracing relay optics image formation

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Correction accuracy in image-based adaptive optics for nonlinear microscopy

Correction accuracy in image-based adaptive optics for nonli...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Facomprez, Aurelie Beaurepaire, Emmanuel Debarre, Delphine Ecole Polytech ParisTech Lab Opt & Biosci CNRS F-91128 Palaiseau France INSERM F-91128 Palaiseau France

ISBN: (纸本)9780819488701

We investigate the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. In this paper we focus on the case of two-photon excited fluorescence. Using analytical, numerical and experimental results, we show that using a suitable number of measurements, accurate correction can be achieved for up to 2 rad rms initial aberrations even without optimisation of the correction modes. We demonstrate that this correction can be achieved using low light levels to minimise photobleaching and toxicity, and we provide examples of such optimised correction.

关键词： Adaptive optics aberration correction multiphoton microscopy nonlinear microscopy

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Improved optical method for measuring of AFM cantilever deflection

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Baibyrin, VB Konnov, NP Volkov, UP Saratov Tech Univ Saratov 410016 Russia

ISBN: (纸本)0819427004

In our lab have been developed an atomic force microscope (AFIM) for biological and technical applications with improved optical method for measuring of cantilever deflection. We use conventional technique of measuring the change in angle of light reflected off the cantilever with an additional lens placed between cantilever and four-segment photodiode, The lens forms an image of the cantilever in the photodiode plane. Small sizes of the cantilever image improve the resolution of AFM and reduce the requirements to the optical scheme of the microscope. The lens non-linear transmission of the cantilever deflection is compensated by means of computer program. With the A FM were imaged the DNA of plague microbes (Y. pestis EV) and phages of plague and V. Cholera.

关键词： atomic force microscopy cantilever deflection methods phage DNA biology

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Optical characterization of microfabricated solid immersion lenses

Optical characterization of microfabricated solid immersion ...

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Fletcher, DA Crozier, KB Goodson, KE Quate, CF Kino, GS Stanford Univ Ginzton Lab Stanford CA 94305 USA

ISBN: (纸本)081943535X

Circumventing the optical diffraction limit is important for data storage, photolithography, and imaging. The Solid Immersion Lens (SIL), in which light is focussed through a high refractive-index lens held close to a sample, offers a method for improving resolution. Microfabrication of SILs enables the use of lenses as small as a few wavelengths in diameter. When these are integrated onto cantilevers, the SIL can be scanned over topography with control of lens-sample separation. We have extended Mie theory to investigate aberration in small lenses and the effect of lens size on a converging beam. We also report progress on the fabrication and first results from micromachined silicon SILs.

关键词： microlens microfabrication solid immersion lens scanning probe microscopy

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Multispectral time-resolved fluorescence microscopy based on compressive acquisitions 29

Multispectral time-resolved fluorescence microscopy based on...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXix

作者： Ghezzi, Alberto Farina, Andrea Valentini, Gianluca Bassi, Andrea D'Andrea, Cosimo Politecn Milan Dipartimento Fis Piazza L da Vinci 32 I-20133 Milan Italy CNR IFN Piazza L da Vinci 32 I-20133 Milan Italy Ist Italiano Tecnol Ctr NanoSci & Technol PoliMi Via Pascoli 70-3 I-20133 Milan Italy

ISBN: (纸本)9781510648043;9781510648036

multidimensional fluorescence microscopy techniques produce dataset rich of information (space, emission spectrum and lifetime) to investigate photophysical processes in biological samples. To acquire a 4D dataset, one promising microscope design is based on the single-pixel camera scheme and on compressive sensing acquisitions, thanks to which the measurement time can be reduced. Within this framework, a computational step is required to move from the acquisition space to the pixel space and, subsequently, the analysis can be carried out exploiting the high dimensionality. In this work we present an experimental system and a fast-fit method that can produce a map of fluorophore concentrations in parallel to the measurement routine.

关键词： fluorescence microscopy compressive sensing multispectral imaging time-resolved imaging

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