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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

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Lensfree on-chip high-resolution imaging using two-way lighting, and its limitations 13

Lensfree on-chip high-resolution imaging using two-way light...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Adachi, Yasuhiko Tamaki, Tokuhiko Motomura, Hideto Kato, Yoshihisa Panasonic Corp Adv Res Div Osaka Japan Panasonic Corp Automot & Ind Syst Co Div Engn Kyoto Japan

ISBN: (纸本)9781628419474

A high-magnification image of a biological sample can generally be obtained by an optical microscope with an objective lens, moving the image sensor with a sub-pixel shift and the subsequent image processing for super-resolution. However, to obtain a high-resolution image, a large number of images will be required for the super-resolution, and thus it is difficult to achieve real-time operation, and the field-of-view (FOV) is not sufficiently wide. The currently proposed digital holography technique places a sample on the image sensor and captures the interference fringe (hologram) to reconstruct a 3D high-resolution image in a computer. This technique ensures the features of a wide FOV, whereas the high resolution obtained by image processing cannot ensure real-time operation, because it requires recursive calculations of light propagation and adequate computer resources. To realize wide FOV and the real-time operation at the same time, we have developed a new technique: Lensfree on-chip high-resolution imaging using two-way lighting. High-resolution image is immediately obtained by image processing of the low-resolution images of the samples. This makes it possible to ensure a wide FOV, a deep depth of focus without the need for focus adjustment, and a continuously expanding operation. We also discuss the limitations of the high resolution.

关键词： Lensfree microscopy On-chip microscopy Two-way lighting

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microscopy using source and detector arrays 13

Microscopy using source and detector arrays

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Sheppard, Colin J. R. Castello, Marco Vicidomini, Giuseppe Duocastella, Marti Diaspro, Alberto Ist Italiano Tecnol Nanoscopy Nanophys Via Morego 30 I-16163 Genoa Italy Univ Genoa DIBRIS Via Opera Pia 13 I-16145 Genoa Italy

ISBN: (纸本)9781628419474

There are basically two types of microscope, which we call conventional and scanning. The former type is a full-field imaging system. In the latter type, the object is illuminated with a probe beam, and a signal detected. We can generalize the probe to a patterned illumination. Similarly we can generalize the detection to a patterned detection. Combining these we get a range of different modalities: confocal microscopy, structured illumination (with full-field imaging), spinning disk (with multiple illumination points), and so on. The combination allows the spatial frequency bandwidth of the system to be doubled. In general we can record a four dimensional (4D) image of a 2D object (or a 6D image from a 3D object, using an acoustic tuneable lens). The optimum way to directly reconstruct the resulting image is by image scanning microscopy (ISM). But the 4D image is highly redundant, so deconvolution-based approaches are also relevant. ISM can be performed in fluorescence, bright field or interference microscopy. Several different implementations have been described, with associated advantages and disadvantages. In two-photon microscopy, the illumination and detection point spread functions are very different. This is also the case when using pupil filters or when there is a large Stokes shift.

关键词： Confocal microscopy scanning optical microscopy fluorescence microscopy image scanning microscopy pixel reassignment

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Novel contrast mechanism for label free super-resolution imaging 13

Novel contrast mechanism for label free super-resolution ima...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Alexandrov, Sergey A. McGrath, James Subhash, Hrebesh Boccafoschi, Francesca Giannini, Cinzia Leahy, Martin Natl Univ Ireland Sch Phys Tissue Opt & Microcirculat Imaging Grp Galway Ireland Univ Piemonte Orientale Dept Hlth Sci I-28100 Novara Italy CNR Inst Crystallog Via Amendola 122-O I-70126 Bari Italy

ISBN: (纸本)9781628419474

We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form superresolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.

关键词： Optical microscopy label-free imaging super-resolution

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Confocal imaging with orthogonally polarized illumination beams 13

Confocal imaging with orthogonally polarized illumination be...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Kalita, Ranjan Boruah, Bosanta R. Indian Inst Technol Guwahati Dept Phys Gauhati Assam India

ISBN: (纸本)9781628419474

In confocal microscopy the polarization of the illumination beam plays an important role in determining the orientation of the fluorescent molecules being illuminated. The efficiency of the excitation depends on the angle between the excitation electric field and the direction of the molecular dipole. In order to determine the orientation of the fluorescent molecules in the focal plane the molecules are to be excited using two mutually orthogonal electric fields. In this paper we show how a computer generated holography technique can be implemented using a ferroelectric liquid crystal spatial light modulator to conveniently obtain two images of the same target once with an X polarized illumination beam and another with a Y polarized illumination beam.

关键词： Confocal microscopy Computer generated holography Polarization Molecular orientation

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Super-resolution optical microscopy by using dielectric microwires 13

Super-resolution optical microscopy by using dielectric micr...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Darafsheh, Arash Wu, Gaoxiang Yang, Shu Finlay, Jarod C. Univ Penn Dept Radiat Oncol Philadelphia PA 19104 USA Univ Penn Dept Mat Sci & Engn 3231 Walnut St Philadelphia PA 19104 USA

ISBN: (纸本)9781628419474

We demonstrate that super-resolution imaging of specimens containing sub-diffraction-limited features is feasible by using dielectric microwires fabricated through capillary force lithography followed by photopatterning. As supplementary micron scale cylindrical lenses, we fabricated uniform-sized microwires with and 5 and 10 mu m diameters and refractive index similar to 1.3-1.6. The microwires are placed in contact with the specimen to collect the information of the sub-wavelength features of the specimen and transmit them to the far-field with magnification enabling imaging with two-fold resolution improvement. Potential applications of our imaging technique include biological imaging, microfluidics, and nanophotonics applications.

关键词： Super-resolution microscopy nanoscopy microwire

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Application of linear-scale differential analysis in phase correlation method of image stitching 13

Application of linear-scale differential analysis in phase c...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Bezzubik, Vitalii V. Belashenkov, Nickolai R. Losev, Sergei V. ITMO Univ Kronverksky Pr 49 St Petersburg 197101 Russia

ISBN: (纸本)9781628419474

A robust method of creation of panoramic images which does not consume much processing resources is proposed and investigated. The phase correlation (PC) method is taken as a basic one because of simplicity of its algorithm and low computing time due to application of FFT technique. Standard PC procedure is modified by preprocessing of source frames of panoramic images in spatial domain. Preprocessing comprises Linear-Scale Differential Analysis (LSDA) with sequent content-dependent thresholding of intensity gradients. Method is proved for artificially blurred and noise corrupted images. It is shown that new robust algorithm allows to increase the productivity of creation of panoramic images keeping the probability of successive stitching close to maximum even for low-quality source frames.

关键词： Digital image image stitching phase correlation linear-scale differential analysis

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Modified K-factor image decomposition for three-dimensional super resolution microscopy 13

Modified K-factor image decomposition for three-dimensional ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Ilovitsh, Tali Weiss, Aryeh Meiri, Amihai Ebeling, Carl G. Amiel, Aliza Katz, Hila Mannasse-Green, Batya Zalevsky, Zeev Bar Ilan Univ Fac Engn IL-52900 Ramat Gan Israel Univ Utah Dept Elect & Comp Engn Salt Lake City UT USA Univ Utah Dept Phys & Astron Salt Lake City UT USA Meir Med Ctr Genet Inst Kefar Sava Israel Bar Ilan Univ Fac Life Sci Ramat Gan Israel

ISBN: (纸本)9781628419474

The ability to track single fluorescent particles within a three dimensional (3D) cellular environment can provide valuable insights into cellular processes. In this paper, we present a modified nonlinear image decomposition technique called K-factor that reshapes the 3D point spread function (PSF) of an XYZ image stack into a narrow Gaussian profile. The method increases localization accuracy by similar to 60% with compare to regular Gaussian fitting, and improves minimal resolvable distance between overlapping PSFs by similar to 50%. The algorithm was tested both on simulated data and experimentally. This work presets a novel use of the nonlinear image decomposition technique called K-factor that reshapes the three dimensional (3D) point spread function (PSF) of an XYZ image stack into a narrow Gaussian profile. The experimentally obtained PSF of a Z-stack raw data that is acquired by a widefield microscope has a more elaborate shape that is given by the Gibson and Lanni model. This shape increases the computational complexity associated with the localization routine, when used in localization microscopy techniques. Furthermore, due to its nature, this PSF spreads over a larger volume, making the problem of overlapping emitters detection more pronounced. The ability to use Gaussian fitting with high accuracy on 3D data can facilitate the computational complexity, hence reduce the processing time required for the generation of the 3D superresolved image. In addition it allows the detection of overlapping PSFs and reduces the effects of the penetration of out of focus PSFs into in focused PSFs, therefore enables the increase in the activated fluorophore density by similar to 50%. The algorithm was tested both on simulated data and experimentally, where it yielded an increase in the localization accuracy by similar to 60% with compare to regular Gaussian fitting, and improved the minimal resolvable distance between overlapping PSFs by similar to 50%, making it extrem

关键词： Three-dimensional microscopy image processing Fluorescence Superresolution

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Design of adaptive objective lens for ultrabroad near infrared imaging 13

Design of adaptive objective lens for ultrabroad near infrar...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Lan, Gongpu Li, Guoqiang Ohio State Univ Dept Ophthalmol & Visual Sci Columbus OH 43212 USA Ohio State Univ Dept Elect & Comp Engn 1330 Kinnear Road Columbus OH 43212 USA

ISBN: (纸本)9781628419474

We present a compound adaptive objective lens in which a water-filled membrane lens is inserted into a front group (one lens) and a back group (two lenses). This adaptive objective lens works in the ultrabroad near infrared waveband (760nm similar to 920nm) with the volume scan of > 1mm(3) and the resolution of 2.8 mu m (calculated at the wavelength of 840 nm). The focal range is 19.5mm similar to 20.5mm and the numerical number is 0.196. The size of the adaptive lens is 10mm (diameter) x 17mm (length). This kind of lens can be widely used in three-dimensional (3D) volume biomedical imaging instruments, such as confocal microscope, optical coherence tomography (OCT), two photon microscope, etc.

关键词： Active optics adaptive objective lens extended depth of field three-dimensional microscopy confocal microscopy optical coherence tomography two-photon microscopy optical design

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A linear algorithm for quantitative measure of corneal collagen fiber orientation using second harmonic generation microscopy 13

A linear algorithm for quantitative measure of corneal colla...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： McLean, James DiMarzio, Charles Northeastern Univ 360 Huntington Ave Boston MA 02115 USA

ISBN: (纸本)9781628419474

There is significant interest in the scientific community to develop a reliable and precise quantification of the direction of collagen fibers within the cornea in order to learn more about how collagen contributes to the cornea's shape and structure. Previous work has shown that quantification of these fibers' orientations is possible using Second Harmonic Generation (SHG) microscopy, a modality that utilizes the non-centrosymmetric properties of collagen to obtain details of their macromolecular structure. Many attempts at using SHG to this purpose result in whole volume approximations which do not take into account variations through the depth of the cornea. Additionaly, other algorithms have used non-linear processes which limit computation time and result in sensitivity to sample area, resolution, and illumination. We propose a linear method for quantification of collagen fiber orientation which utilizes the precise sectioning properties of SHG and is independent of non-linear artifacts to aid in further classification of cornea structure.

关键词： Second Harmonic Generation collagen cornea orientation quantification

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Dual-detection confocal microscopy: high-speed surface profiling without depth scanning 13

Dual-detection confocal microscopy: high-speed surface profi...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Lee, Dong-Ryoung Gweon, Dae-Gab Yoo, Hongki Korea Adv Inst Sci & Technol KAIST Dept Mech Engn Nano Optomechatron Lab 291 Daehak Ro Daejeon 34141 South Korea Hanyang Univ Dept Biomed Engn Biomed Opt & Photomed Lab 222 Wangsimni Ro Seoul 04763 South Korea

ISBN: (纸本)9781628419474

We propose a new method for three-dimensional (3-D) imaging without depth scanning that we refer to as the dual-detection confocal microscopy (DDCM). Compared to conventional confocal microscopy, DDCM utilizes two pinholes of different sizes. DDCM generates two axial response curves which have different stiffness according to the pinhole diameters. The two axial response curves can draw the characteristics curve of the system which shows the relationship between the axial position of the sample and the intensity ratio. Utilizing the characteristic curve, the DDCM reconstructs a 3-D surface profile with a single 2-D scanning. The height of each pixel is calculated by the intensity ratio of the pixel and the intensity ratio curve. Since the height information can be obtained directly from the characteristic curve without depth scanning, a major advantage of DDCM over the conventional confocal microscopy is a speed. The 3-D surface profiling time is dramatically reduced. Furthermore, DDCM can measure 3-D images without the influence of the sample condition since the intensity ratio is independent of the quantum yield and reflectance. We present two types of DDCM, such as a fluorescence microscopy and a reflectance microscopy. In addition, we extend the measurement range axially by varying the pupil function. Here, we demonstrate the working principle of DDCM and the feasibility of the proposed methods.

关键词： Confocal microscopy three-dimensional microscopy high speed imaging

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