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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是131-140 订阅

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Fourier Analysis of Mitochondrial Distribution in Oocytes

Fourier Analysis of Mitochondrial Distribution in Oocytes

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Hollmann, Joseph L. Brooks, Dana H. Newmark, Judith A. Warner, Carol M. DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn Dana Res Ctr 409 360 Huntington Ave Boston MA 02115 USA Northeastern Univ Dept Biol Mugar Life Sci 134 Boston MA 02115 USA Compucyte Boston MA 02115 USA Northeastern Univ Dept Mech & Ind Engn Boston MA 02115 USA

ISBN: (纸本)9780819484413

This paper describes a novel approach to quantifying mitochondrial patterns which are typically described using the qualitative terms "diffuse" "aggregated" and are potentially key indicators for an oocyte's health and survival potential post-implantation. An oocyte was isolated in a confocal image and a coarse grid was superimposed upon it. The spatial spectrum was calculated and an aggregation factor was generated. A classifier for healthy cells was developed and verified. The aggregation factor showed a clear distinction between the healthy and unhealthy oocytes. The ultimate goal is to screen oocytes for viability preimplantation, thus improving the outcome of in vitro fertilization (IVF) treatments.

关键词： Spatial frequencies image analysis Confocal microscopy 3D image processing Algorithm development MATLAB Digital image correlation Microscopes microscopy Visualization

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Multifocus imaging via polarization wavefront shaping 27

Multifocus imaging via polarization wavefront shaping

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Li, Chen Ding, Changqin Li, Minghe Rong, Jiayue Ulcickas, James Simpson, Garth J. Purdue Univ Dept Chem W Lafayette IN 47907 USA

ISBN: (纸本)9781510632547

Conventional three-dimensional (3D) images of biological samples are typically assembled from a stack of two-dimensional images acquired sequentially at different focal planes. This time-consuming manner hinders the application of 3D imaging techniques to the investigation of fast biochemical dynamics and light-sensitive biological events. The concept of multifocus imaging, which enables simultaneous acquisition of images from multiple focal planes, was introduced to achieve rapid 3D imaging. In the present study, we achieved multifocus imaging through polarization wavefront shaping via a micro-retarder array which splits the incident linearly polarized light into three beamlets that are focused to three axially-offset focal planes with 100 itm separation. Append to an existing beam-scanning microscope, this multifocus system enables rapid 3D imaging compatible with a variety of optical microscopic approaches including laser transmittance, two-photon excited fluorescence, and second harmonic generation microscopy.

关键词： Multifocus imaging beam scanning microscopy polarization wavefront shaping Jones calculus

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Three-dimensional imaging with a quadrature tomographic microscope

Three-dimensional imaging with a quadrature tomographic micr...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Stott, JJ Bennett, RE Warner, CM DiMarzio, CA Northeastern Univ Ctr Subsurface Sensing & Imaging Syst Boston MA 02115 USA

ISBN: (纸本)0819439398

The quadrature tomographic microscope combines conventional microscopy with interferometric quadrature detection techniques to yield a simultaneous measurement of both the amplitude and phase of the electric field. This is of tremendous utility in imaging nearly-transparent objects since changes in phase can be directly attributed to changes in the object's index of refraction while the small amplitude changes provide additional structural information. In this paper we will discuss the progress we have made towards reconstructing three dimensional images from the measured two-dimensional complex electric field. BS applying an artificial curvature to the measured field and back-propagating the Gaussian beams, we are able to digitally manipulate the imaging kernel and image different focal planes.

关键词： microscopy optical quadrature tomography quantitative biology

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Real-time dual-wavelength digital holographic microscopy for extended measurement range with enhanced axial resolution

Real-time dual-wavelength digital holographic microscopy for...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Kuehn, Jonas Colomb, Tristan Pache, Christophe Charriere, Florian Montfort, Frederic Cuche, Etienne Emery, Yves Marquet, Pierre Depeursinge, Christian Ecole Polytech Fed Lausanne Inst Imaging & Appl Opt CH-1015 Lausanne Switzerland CHUV Dept Psychiat Ctr Neurosci Psychiat CH-1008 Prilly Switzerland Lyncee Tec SA PSE A CH-1015 Lausanne Switzerland

ISBN: (纸本)9780819470362

We report on advanced dual-wavelength digital holographic microscopy (DHM) methods, enabling single-acquisition real-time micron-range measurements while maintaining single-wavelength interferometric resolution in the nanometer regime. In top of the unique real-time capability of our technique, it is shown that axial resolution can be further increased compared to single-wavelength operation thanks to the uncorrelated nature of both recorded wavefronts. It is experimentally demonstrated that DHM topographic investigation within 3 decades measurement range can be achieved with our arrangement, opening new applications possibilities for this interferometric technique.

关键词： digital holography dual-wavelength two-wavelengths beat-wavelength phase microscopy

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Microlymphatic flow using fast video microscopy

Microlymphatic flow using fast video microscopy

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Dixon, JB Zawieja, DC Gashev, AA Coté, GL Texas A&M Univ Dept Biomed Engn College Stn TX 77843 USA

ISBN: (纸本)0819456756

Despite advances in the measurement of lymphatic flow, little is known about the actual velocities of flow in microlymphatic (similar to 100 um diameter) vessels. In this paper, video microscopy and particle tracking methods were adapted and integrated with an ultra high-speed imaging camera to obtain measurements of high-speed lymph velocities that previous systems were incapable of measuring. In this study, a mesenteric microlymphatic vessel in a loop of the small intestine of a male Sprague-Dawley rat was exteriorized and imaged at a rate of 500 frames per second (fps) for several contraction sequences. Lymph velocity was shown to fluctuate cyclically with the vessel wall contractions and ranged from -1 to 4 mm/sec through a ten second sequence.

关键词： Lymphatic system Cameras Velocity measurements Video microscopy Microfluidics Imaging systems High speed cameras Microfluidic imaging Video Particles

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High-contrast 3D microscopic imaging of deep layers in a biological medium

High-contrast 3D microscopic imaging of deep layers in a bio...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Faridian, Ahmad Pedrini, Giancarlo Osten, Wolfgang Univ Stuttgart Inst Tech Opt SCoPE Pfaffenwaldring 9 D-70569 Stuttgart Germany

ISBN: (纸本)9780819498625

Multilayer imaging of biological specimens is a demanding field of research, but scattering is one of the major obstacles in imaging the internal layers of a specimen. Although in many studies the biological object is assumed to be a weak scatterer, this condition is hardly satisfied for sub-millimeter sized organisms. The scattering medium is inhomogeneously distributed inside the specimen. Therefore, the scattering which occurs in the upper layers of a given internal layer of interest is different from the lower layers. That results in a different amount of collectable information for a specific point in the layer from each view. An opposed view dark-field digital holographic microscope (DHM) has been implemented in this work to collect the information concurrently from both views and increase the image quality. Implementing a DHM system gives the possibility to perform digital refocusing process and obtain multilayer images from each side without depth scanning of the object. The results have been presented and discussed here for a Drosophila embryo.

关键词： Digital holographic microscopy Multilayer imaging Three-dimensional microscopy image enhancement image fusion

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Microscopic tomography by digital interference holography

Microscopic tomography by digital interference holography

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Kim, MK Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819452327

We are developing the digital interference holography (DIH) as a novel method of microscopic tomographic imaging by numerical superposition of a number of holographic fields taken with varying wavelengths. The digital interference holography does not involve pixel-by-pixel mechanical scanning of three-dimensional volume and yet achieves high lateral and longitudinal resolutions. The holographic interference pattern of an ob ect is generated optically and recorded digitally using a CCD camera. The hologrpahic image field is numerically calculated using basic diffraction formulas and the process is repeated for a range of varying wavelengths at regular intervals. Numerical superposition, or digital interference, of the holographic image fields yields the desired three-dimensional representation of the object. Experiments have demonstrated a few-micron lateral and axial resolutions. Furthermore, since the DIH is a coherent imaging system, one can form true tomographic images of sub-surface structures, in the presence of diffuse scattering from overlying layers. By being able to generate true tomographic images of subsurface structures, without the need for three-dimensional mechanical scannig, the DIH method can provide a very efficient and versatile imaging modality for a wide range of applications in materials science and biomedical imaging.

关键词： digital holography tomography microscopy multi-wavelength imaging

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High speed phase distortion measurement and compensation for focusing in space and time

High speed phase distortion measurement and compensation for...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Fiolka, Reto Cui, Meng Howard Hughes Med Inst Ashburn VA 20147 USA

ISBN: (纸本)9780819493583

Random scattering and aberrations severely limit the imaging depth in optical microscopy. We introduce a rapid, parallel wavefront compensation technique that efficiently compensates even highly complex phase distortions. Using coherence gated backscattered light as a feedback signal, we focus light deep inside highly scattering brain tissue. We demonstrate that the same wavefront optimization technique can also be used to compensate spectral phase distortions in ultrashort laser pulses using nonlinear iterative feedback. We can restore transform limited pulse durations at any selected target location and compensate for dispersion that has occurred in the optical train and within the sample.

关键词： Fluorescence microscopy adaptive optics imaging through turbid media ultrafast optics

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Optically Sectioned Imaging by Oblique Plane microscopy

Optically Sectioned Imaging by Oblique Plane Microscopy

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Kumar, Sunil Lin, Ziduo Lyon, Alex R. MacLeod, Ken T. Dunsby, Chris Univ London Imperial Coll Sci Technol & Med Dept Phys Photon Grp S Kensington Campus London SW7 2AZ England Imperial Coll London Natl Heart & Lung Inst London W2 1NY England Imperial Coll London Natl Heart & Lung Inst London SW3 6LY England Imperial Coll London Dept Med Div Med London W12 0HS England

ISBN: (纸本)9780819484413

Oblique Plane microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. The first OPM results obtained using a high NA water immersion lens on a commercially available inverted microscope frame are presented, together with a measurement of the achievable optical resolution.

关键词： Fluorescence microscopy Three-dimensional microscopy Selective Plane Illumination microscopy

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Fluorescence microscopy with isotropic resolution using three objectives 13

Fluorescence microscopy with isotropic resolution using thre...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Huelsnitz, Thomas Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA

ISBN: (纸本)9781628419474

Widefield and confocal fluorescence microscopy using a single objective suffer from poor resolution and a strong anisotropy between the lateral and axial resolution. Coherently combining the excitation and emission from two coaxial objectives improves the axial resolution up to sevenfold, but leaves the lateral resolution unchanged. Here we investigate the coherent combination of three objectives to create a point spread function (PSF) that is isotropic with higher resolution in the plane of the objectives. We develop a theoretical framework for simulating the performance of interferometric imaging with three objectives. Using three identical objectives with a large working distance and 0.9 numerical aperture (NA), the full-width half maximum of the confocal PSF is 135 nm compared to the lateral FWHM of 274 nm for imaging with a single objective at a wavelength of 515 nm.

关键词： microscopy fluorescence microscopy interferometric imaging PSF Engineering

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