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Tomographic phase microscopy using optical tweezers 4

Tomographic phase microscopy using optical tweezers

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conference on Advanced microscopy Techniques IV and Neurophotonics II

作者： Habaza, Mor Gilboa, Barak Roichman, Yael Shaked, Natan T. Tel Aviv Univ Dept Biomed Engn IL-69978 Tel Aviv Israel Tel Aviv Univ Dept Exact Sci IL-69978 Tel Aviv Israel

ISBN: (纸本)9781628417012

We review our technique for tomographic phase microscopy with optical tweezers [1]. This tomographic phase microscopy approach enables full 3-D refractive-index reconstruction. Tomographic phase microscopy measures quantitatively the 3-D distribution of refractive-index in biological cells. We integrated our external interferometric module with holographic optical tweezers for obtaining quantitative phase maps of biological samples from a wide range of angles. The close-to-common-path, off-axis interferometric system enables a full-rotation tomographic acquisition of a single cell using holographic optical tweezers for trapping and manipulating with a desired array of traps, while acquiring phase information of a single cell from all different angles and maintaining the native surrounding medium. We experimentally demonstrated two reconstruction algorithms: the filtered back-projection method and the Fourier diffraction method for 3-D refractive index imaging of yeast cells.

关键词： Holography Interference microscopy Tomography Optical tweezers image reconstruction techniques Three dimensional image processing

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OPTIMIZATION AND DEVELOPMENT OF X-RAY microscopy TECHNIQUE FOR INVESTIGATION OF THERMAL BARRIER COATING

OPTIMIZATION AND DEVELOPMENT OF X-RAY MICROSCOPY TECHNIQUE F...

引用

conference and Exhibition on Materials Science and Technology (MS and T)

作者： Asadizanjani, Navid Shahbazmohammadi, Sina Jordan, Eric H. Univ Connecticut Storrs CT 06269 USA

ISBN: (纸本)9781119183853;9781119183877

Full understanding of the cracking behavior of thermal barrier coatings (TBCs), requires a three-dimensional (3D) characterization of all layers. Common microscopy techniques are based on 2D cross section images and fail provide 3D information and because such methods are destructive it is not possible to watch the growth or linking of specific cracks. Collecting a series of two-dimensional (2D) cross-section images to generate the 3D volume is both time consuming and will not allow tracking the growth of cracks but instead provides only statistical information that also relies of assuming all samples behave the same way. X-ray tomography or micro-CT is as a valuable nondestructive method which can potentially provide us with the unique opportunity to track changes of the same location and hence same crack as a function of time during thermal cycling. X-ray tomography presents a number of challenges including the relatively high attenuation of x-rays in the TBC and substrate. There are various nontrivial parameters yet to be optimized for a successful tomography. Sample size and several interdependent image acquisition parameters are the most significant factors determining the quality of imaging. Also, it is necessary to achieve repeatable image processing and develop algorithms that can extract quantitative information;otherwise, X-ray microscopy remains merely qualitative. Here we seek to establish a reliable procedure to acquire meaningful repeatable quantitative 3D information of TBC samples. It is shown that parameters have to be optimized based on the region of interest in TBC samples, that is, there are different parameters to be tuned for the TGO thickness measurement, recording of interfacial surface geometry change, evolution of cracks in the ceramic top coat and study of bond coat compositional integrity. These optimized parameters are explained and verified. In addition, a sample analysis with a repeatability check has also been performed to ensure the

关键词： Cracks

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Towards automated firearm identification based on high resolution 3D data: Rotation-invariant features for multiple line-profile-measurement of firing pin shapes

Towards automated firearm identification based on high resol...

引用

conference on Three-dimensional image processing, Measurement (3DIPM), and Applications

作者： Fischer, Robert Vielhauer, Claus Brandenburg Univ Appl Sci Dept Informat & Media D-14770 Brandenburg Germany Otto Von Guericke Univ Res Grp Multimedia & Secur D-39106 Magdeburg Germany

ISBN: (纸本)9781628414837

Understanding and evaluation of potential evidence, as well as evaluation of automated systems for forensic examinations currently play an important role within the domain of digital crime scene analysis. The application of 3D sensing and pattern recognition systems for automatic extraction and comparison of firearm related toolmarks is an evolving field of research within this domain. In this context, the design and evaluation of rotation-invariant features for use on topography data play a particular important role. In this work, we propose and evaluate a 3D imaging system along with two novel features based on topography data and multiple profile-measurement-lines for automatic matching of firing pin shapes. Our test set contains 72 cartridges of three manufactures shot by six different 9mm guns. The entire pattern recognition workflow is addressed. This includes the application of confocal microscopy for data acquisition, preprocessing covers outlier handling, data normalization, as well as necessary segmentation and registration. Feature extraction involves the two introduced features for automatic comparison and matching of 3D firing pin shapes. The introduced features are called 'Multiple-Circle-Path' (MCP) and 'Multiple-Angle-Path' (MAP). Basically both features are compositions of freely configurable amounts of circular or straight path-lines combined with statistical evaluations. During the first part of evaluation (E-1), we examine how well it is possible to differentiate between two 9mm weapons of the same mark and model. During second part (E-2), we evaluate the discrimination accuracy regarding the set of six different 9mm guns. During the third part (E-3), we evaluate the performance of the features in consideration of different rotation angles. In terms of E-1, the best correct classification rate is 100% and in terms of E-2 the best result is 86%. The preliminary results for E-3 indicate robustness of both features regarding rotation. However, in fu

关键词： 3D image processing 3D shape indexing and retrieval feature extraction pattern recognition line-profile-measurement rotation invariance digital crime scene analysis firearm identification

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Phase mask optimization for 3D parallax EDF microscopy

Phase mask optimization for 3D parallax EDF Microscopy

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Beckers, Ingeborg E. Gierlak, Michael Hoeppel, Robert Landskron, Juergen Beuth Univ Appl Sci Berlin Dept Appl Phys Med Engn D-13347 Berlin Germany

ISBN: (纸本)9780819498625

Extended depth-of-field (EDF) microscopy is a well-investigated and very simple method to obtain projection images with an extended depth of focus. Despite its advantages of being a real-time method applicable to any microscopic mode with high lateral resolution that can be simply realized by extending a commercial microscope, the lack of z-correlation is still a problem. In this work we present a combined technique of EDF and stereomicroscopy. By cross-correlation depth information is obtained. Finally, 3D images are reconstructed for best phase masks and simulation results are evaluated experimentally.

关键词： 3D imaging real time imaging extended depth of field microscopy EDF space light modulation SLM stereoscopy parallax EDFP

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Spectral-domain interferometry for quantitative DIC microscopy

Spectral-domain interferometry for quantitative DIC microsco...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Li, Chengshuai Zhu, Yizheng Virginia Tech Bradley Dept Elect & Comp Engn Blacksburg VA 24060 USA

ISBN: (纸本)9780819498625

A spectral-domain differential interference contrast (SD-DIC) microscopy system is presented for quantitative imaging of both reflective and transparent samples. The spectral-domain interferometry, combined with the common-path DIC geometry, provides a shot noise-limited sensitivity of 14.3pm in optical pathlength gradient measurement. The optical resolution of the system was characterized using images of a USAF resolution target. Fused silica microspheres were imaged to demonstrate the reconstruction of two-dimensional optical pathlength topography from measured gradient fields. The exquisite sensitivity of the system showed potential in quantitative imaging of sub-diffraction limit objects such as gold nanoparticles.

关键词： Differential interference contrast quantitative phase imaging spectral-domain interferometry

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Agile scanning using a MEMS focus control mirror in a commercial confocal microscope

Agile scanning using a MEMS focus control mirror in a commer...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Lukes, Sarah J. Dickensheets, David L. Montana State Univ Dept Elect & Comp Engn Bozeman MT 59717 USA

ISBN: (纸本)9780819498625

We present the use of a large stroke deformable membrane mirror for focus control over a range of 123 mu m in a commercial confocal microscope at 0.5-0.64 NA. The MEMS mirror is much faster than lens or stage translation allowing synchronization to x-y scanning for agile control over the 3D shape of the surface that is scanned. We describe a novel, low-actuator-count MEMS mirror designed specifically for focus control in scanning laser microscopes and present images taken with the mirror deployed on an Olympus Fluoview FV300.

关键词： confocal microscopy deformable membrane mirror MEMS MEOMS focus control aberration compensation

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High-contrast 3D microscopic imaging of deep layers in a biological medium

High-contrast 3D microscopic imaging of deep layers in a bio...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Faridian, Ahmad Pedrini, Giancarlo Osten, Wolfgang Univ Stuttgart Inst Tech Opt SCoPE Pfaffenwaldring 9 D-70569 Stuttgart Germany

ISBN: (纸本)9780819498625

Multilayer imaging of biological specimens is a demanding field of research, but scattering is one of the major obstacles in imaging the internal layers of a specimen. Although in many studies the biological object is assumed to be a weak scatterer, this condition is hardly satisfied for sub-millimeter sized organisms. The scattering medium is inhomogeneously distributed inside the specimen. Therefore, the scattering which occurs in the upper layers of a given internal layer of interest is different from the lower layers. That results in a different amount of collectable information for a specific point in the layer from each view. An opposed view dark-field digital holographic microscope (DHM) has been implemented in this work to collect the information concurrently from both views and increase the image quality. Implementing a DHM system gives the possibility to perform digital refocusing process and obtain multilayer images from each side without depth scanning of the object. The results have been presented and discussed here for a Drosophila embryo.

关键词： Digital holographic microscopy Multilayer imaging Three-dimensional microscopy image enhancement image fusion

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Detecting jaundice by using digital image processing

Detecting jaundice by using digital image processing

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Castro-Ramos, J. Toxqui-Quitl, C. Villa Manriquez, F. Orozco-Guillen, E. Padilla-Vivanco, A. Sanchez-Escobar, J. J. Inst Nacl Astrofis Opt & Electr Puebla Mexico Univ Politecn Tulancingo Tulancingo Mexico Univ Politecn Sinaloa Mazatlan Mexico Ctr Ensenanza Tecn Ind Guadalajara Jalisco Mexico

ISBN: (纸本)9780819498625

When strong Jaundice is presented, babies or adults should be subject to clinical exam like "serum bilirubin" which can cause traumas in patients. Often jaundice is presented in liver disease such as hepatitis or liver cancer. In order to avoid additional traumas we propose to detect jaundice (icterus) in newborns or adults by using a not pain method. By acquiring digital images in color, in palm, soles and forehead, we analyze RGB attributes and diffuse reflectance spectra as the parameter to characterize patients with either jaundice or not, and we correlate that parameters with the level of bilirubin. By applying support vector machine we distinguish between healthy and sick patients.

关键词： Digital image processing Diffuse Reflectance Bilirubin Support Vector Machine

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Amplitude, phase, and polarization control with a single spatial light modulator

Amplitude, phase, and polarization control with a single spa...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Theisen, Michael J. Head, Stephen T. Brown, Thomas G. Gillmer, Steven R. Ellis, Jonathan D. Univ Rochester Inst Opt Rochester NY 14627 USA Univ Rochester Dept Mech Engn Rochester NY 14627 USA

ISBN: (纸本)9780819498625

Point spread function engineering is usually accomplished by controlling the amplitude, phase and/or polarization of the pupil fields. We analyze and test an optical design for full amplitude, phase, and polarization control of the pupil fields using a single spatial light modulator. In our scheme, the beam is spatially split into four components whose relative phases provide the four degrees of freedom necessary for amplitude, phase, and polarization control.

关键词： Spatial Light Modulators Polarization Control microscopy Pupil Engineering

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Membrane-substrate separation distance assessed by normalized total internal reflection fluorescence microscopy

Membrane-substrate separation distance assessed by normalize...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Dos Santos, Marcelina Cardoso Vezy, Cyrille Jaffiol, Rodolphe Univ Technol Troyes CNRS UMR 6281 Lab Nanotechnol & Instrumentat OptInst Charles D F-10004 Troyes France

ISBN: (纸本)9780819498625

As a consequence of the recent progress in nanoscale technology, more and more sensitive methods are developed to characterize and understand the dynamic of cell membrane adhesion process. In this paper we present a new quantitative method to measure the separation distances between the membrane and the substrate. This technique is based on a normalization of Total Internal Reflection Fluorescence (TIRF) images by usual epi-illumination images. This simple method allows to achieve a nanometric axial resolution, typically 10 nm. We demonstrate the potential of our technique through the study of phospholipids membranes such as Giant Unilamellar Vesicles (GUVs), which are usual biomimetic systems to investigate membrane-substrate interactions.

关键词： Total Internal Fluorescence (TIRF) nanoscale fluorescence imaging cell adhesion Giant Unilamellar Vesicles (GUV)

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