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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是141-150 订阅

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Extended depth-of-field microscopy

Extended depth-of-field microscopy

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Smith, Christopher Botcherby, Edward Booth, Martin Juskaitis, Rimas Wilson, Tony Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)9780819479662

In this paper, we present details of a scanning two-photon fluorescence microscope we have built with a near-isotropic scan rate. This means that the focal spot can be scanned at high speed along any direction in the specimen, without introducing systematic aberrations. We present experimental point spread function measurements for this system using an Olympus 1.4 NA 60X oil immersion lens that demonstrates an axial range of operation greater than 70 mu m. We give details of a novel actuator device used to displace the focusing element and demonstrate axial scan responses up to 3.5 kHz. Finally, we present an application of this system in liquid crystal research to image the dynamic response of a nematic device during switching. Information about the director field at different levels in the device can be inferred from images acquired with a temporal resolution of 2.5 ms.

关键词： optical refocusing two-photon microscopy

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Progress in Biomedical Optics and Imaging - Three-dimensional and multidimensional microscopy: image acquisition and processing XII

Progress in Biomedical Optics and Imaging - Three-Dimensiona...

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Three- dimensional and Multidomensional microscopy: image acquisition and processing XII

The proceedings contain 23 papers from the conference on Progress in Biomedical Optics and Imaging - Three-dimensional and multidimensional microscopy: image acquisition and processing XII. The topics discussed include: using out-of-focus light to improve image acquisition time in confocal microscopy;three-dimensional optical computed-tomography microscope;image estimation for structured-illumination microscopy;microlymphatic flow using fast video microscopy;confocal reflectance theta line-scanner for imaging tissues in vivo;multimodal optical microscope for imaging biological systems;spectral domain phase microscopy: a new tool for measuring cellular dynamics and cytoplasmic flow;and an algorithm for image stitching and blending.

关键词： Imaging systems

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Three-dimensional limited-angle microtomography of blood cells: experimental results

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Levin, GG Vishnyakov, GN Zakarian, CS Likhachov, AV Pickalov, VV Kozinets, GI Novoderzhkina, JK Streletskaya, EA Russian Res Inst Optophys Measurements Moscow 119361 Russia

ISBN: (纸本)0819427004

Tomographic measurements of the 3D refractive index spatial distribution within optically transparent phase samples with computerized interferometric microscopes are proposed. Phase shifting interferometric microtomography applications for the 3D image reconstruction of the blood cells (lymphocytes) are represented. The immersion 100x, N.A.=1.25 objective was used to increase the spatial resolution and observation angle range to 90 degree. ART, combined ART and iterative Gerchberg-Papoulis 3D algorithm were used for the tomogram reconstruction. To determine the accuracy and spatial resolution of the blood cells image reconstruction by means of the interferometric microtomographic method the numerical simulations were implemented.

关键词： microtomography interferometric microscope refractive index living cells and lymphocyte

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High speed phase distortion measurement and compensation for focusing in space and time

High speed phase distortion measurement and compensation for...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Fiolka, Reto Cui, Meng Howard Hughes Med Inst Ashburn VA 20147 USA

ISBN: (纸本)9780819493583

Random scattering and aberrations severely limit the imaging depth in optical microscopy. We introduce a rapid, parallel wavefront compensation technique that efficiently compensates even highly complex phase distortions. Using coherence gated backscattered light as a feedback signal, we focus light deep inside highly scattering brain tissue. We demonstrate that the same wavefront optimization technique can also be used to compensate spectral phase distortions in ultrashort laser pulses using nonlinear iterative feedback. We can restore transform limited pulse durations at any selected target location and compensate for dispersion that has occurred in the optical train and within the sample.

关键词： Fluorescence microscopy adaptive optics imaging through turbid media ultrafast optics

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Fourier Analysis of Mitochondrial Distribution in Oocytes

Fourier Analysis of Mitochondrial Distribution in Oocytes

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Hollmann, Joseph L. Brooks, Dana H. Newmark, Judith A. Warner, Carol M. DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn Dana Res Ctr 409 360 Huntington Ave Boston MA 02115 USA Northeastern Univ Dept Biol Mugar Life Sci 134 Boston MA 02115 USA Compucyte Boston MA 02115 USA Northeastern Univ Dept Mech & Ind Engn Boston MA 02115 USA

ISBN: (纸本)9780819484413

This paper describes a novel approach to quantifying mitochondrial patterns which are typically described using the qualitative terms "diffuse" "aggregated" and are potentially key indicators for an oocyte's health and survival potential post-implantation. An oocyte was isolated in a confocal image and a coarse grid was superimposed upon it. The spatial spectrum was calculated and an aggregation factor was generated. A classifier for healthy cells was developed and verified. The aggregation factor showed a clear distinction between the healthy and unhealthy oocytes. The ultimate goal is to screen oocytes for viability preimplantation, thus improving the outcome of in vitro fertilization (IVF) treatments.

关键词： Spatial frequencies image analysis Confocal microscopy 3D image processing Algorithm development MATLAB Digital image correlation Microscopes microscopy Visualization

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Multifocus imaging via polarization wavefront shaping 27

Multifocus imaging via polarization wavefront shaping

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Li, Chen Ding, Changqin Li, Minghe Rong, Jiayue Ulcickas, James Simpson, Garth J. Purdue Univ Dept Chem W Lafayette IN 47907 USA

ISBN: (纸本)9781510632547

Conventional three-dimensional (3D) images of biological samples are typically assembled from a stack of two-dimensional images acquired sequentially at different focal planes. This time-consuming manner hinders the application of 3D imaging techniques to the investigation of fast biochemical dynamics and light-sensitive biological events. The concept of multifocus imaging, which enables simultaneous acquisition of images from multiple focal planes, was introduced to achieve rapid 3D imaging. In the present study, we achieved multifocus imaging through polarization wavefront shaping via a micro-retarder array which splits the incident linearly polarized light into three beamlets that are focused to three axially-offset focal planes with 100 itm separation. Append to an existing beam-scanning microscope, this multifocus system enables rapid 3D imaging compatible with a variety of optical microscopic approaches including laser transmittance, two-photon excited fluorescence, and second harmonic generation microscopy.

关键词： Multifocus imaging beam scanning microscopy polarization wavefront shaping Jones calculus

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Hyper-spectrum scanning laser optical tomography 25

Hyper-spectrum scanning laser optical tomography

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conference on Three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Chen, Lingling Li, Guiye Li, Yingchao Liu, Lina Liu, Ang Hu, Xuejuan Ruan, Shuangchen Shenzhen Univ Coll Optoelect Engn Shenzhen 518060 Peoples R China

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

We describe a quantitative fluorescence projection tomography technique which measures the three-dimensional fluorescence spectrum in biomedical samples with size up to several millimeters. This is achieved by acquiring a series of hyperspectral images, by using laser scanning scheme, at different projection angles. We demonstrate that this technique provide a quantitative measure of the fluorescence signal by comparing the spectrum and intensity profile of a fluorescent bead phantom and also demonstrate its application to differentiating the extrinsic label and the autofluorescence in a mouse embryo.

关键词： Fluorescence microcopy Three-dimensional microscopy Multi-dimensional imaging Imaging reconstruction techniques Spectrum-resolved imaging

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Mean cell size and collagen orientation from 2D Fourier analysis of confocal laser scanning microscopy and 2 photon fluorescence microscopy on human skin in vivo

Mean cell size and collagen orientation from 2D Fourier anal...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Lucassen, GW Bakker, BLG Neerken, S Hendriks, RFM Philips Res Labs Personal Care Inst NL-5656 AA Eindhoven Netherlands

ISBN: (纸本)0819447641

We present results from 2D Fourier analysis on 3D stacks of images obtained by confocal laser scanning reflectance microscopy (CLSM) and two-photon fluorescence microscopy (2PM) on human skin in vivo. CLSM images were obtained with a modified commercial system (Vivascope1000(TM), Lucid Inc, excitation wavelength 830 nm) equipped with a piezo-focusing element (350 mum range) for depth positioning of the objective tens. 2PM was performed with a specially designed set-up with excitation wavelength 730 nm. Mean cell size in the epidermal layer and structural orientation in the dermal layer have been determined as a function of depth by 2D Fourier analysis. Fourier analysis on microscopic images enables automatic non-invasive quantitative structural analysis (mean cell size and orientation) of living human skin.

关键词： confocal microscopy 2 photon fluorescence microscopy 2D Fourier analysis cell size collagen orientation epidermis

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Comparison of three dimensional transfer function analysis of alternative phase imaging methods

Comparison of three dimensional transfer function analysis o...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Kou, Shan Shan Sheppard, Colin J. R. Natl Univ Singapore Div Bioengn 9 Engn Dr Singapore 117576 Singapore NUS Grad Sch Interact Sci & Engn Singapore 117597 Singapore

ISBN: (纸本)9780819465566

Phase imaging is an invaluable tool for observation of biological sample, especially for living cells, where staining might not be appropriate, or for materials that do not absorb stain. Imaging of phase distributions with high spatial resolution can be used to derive the actual thickness and refractive index variations in the specimen. The detection of very small phase variations enables the detailed structure in the specimen to be revealed. As a result, the development and utilization of various phase imaging modalities have been important aspects of microscopy research. Differential Interference Contrast (DIC) and Quantitative Phase Microcopy (QPM) are based on partially coherent light, thus enabling high-resolution imaging. However, the low coherence requirement prevents the acquisition of quantitative phase data directly. On the other hand, Digital Holography microscopy (DHM) is able to yield quantitative phase information but is compromised on resolution and cannot give full three dimensional (3D) reconstructions. In this paper, we present the 3D theoretical formalism of the above mentioned phase imaging methods with the focus on DHM. A comparative analysis here through visualization of 3D optical transfer functions gives an insight into the behaviors of these phase imaging methods.

关键词： phase imaging 3D optical transfer function

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Photobleaching Property of Confocal Laser Scanning microscopy with Masked Illumination

Photobleaching Property of Confocal Laser Scanning Microscop...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Kim, DongUk Moon, Sucbei Song, Hoseong Yang, Wenzhong Kim, Dug Y. Gwangju Inst Sci & Technol Dept Informat & Commun Kwangju 500712 South Korea

ISBN: (纸本)9780819479662

Confocal laser scanning microscopy (CLSM) has become the tool of choice for high-contrast fluorescence imaging in the study of the three-dimensional and dynamic properties of biological system. However, the high cost and complexity of commercial CLSMs urges many researchers to individually develop low cost and flexible confocal microscopy systems. The high speed scanner is an influential factor in terms of cost and system complexity. Resonant galvo scanners at several kHz have been commonly used in custom-built CLSMs. However, during the repeated illumination for live cell imaging or 3D image formation, photobleaching and image distortion occurred at the edges of the scan field may be more serious than the center due to an inherent property (e. g. sinusoidal angular velocity) of the scan mirror. Usually, no data is acquired at the edges due to large image distortion but the excitation beam is still illuminated. Here, we present the photobleaching property of CLSM with masked illumination, a simple and low cost method, to exclude the unintended excitation illumination at the edges. The mask with a square hole in its center is disposed at the image plane between the scan lens and the tube lens in order to decrease photobleaching and image distortion at the edges. The excluded illumination section is used as the black level of the detected signals for a signal quantizing step. Finally, we demonstrated the reduced photobleaching at the edges on a single layer of fluorescent beads and real-time image acquisition without a standard composite video signal by using a frame grabber.

关键词： Photobleaching confocal laser scanning microscopy video-rate 3D image masked illumination

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